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Evaluation of different lignocellulosic substrates for the production of cellulases and xylanases by the basidiomycete fungi Bjerkandera adusta and Pycnoporus sanguineus

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Abstract

Agricultural waste products are potential resources for the production of a number of industrial compounds, including biofuels. Basidiomycete fungi display a battery of hydrolytic enzymes with prospective use in lignocellulosic biomass transformation, however little work has been done regarding the characterization of such activities. Growth in several lignocellulosic substrates (oak and cedar sawdust, rice husk, corn stubble, wheat straw and Jatropha seed husk) and the production of cellulases and xylanases by two basidiomycete fungi: Bjerkandera adusta and Pycnoporus sanguineus were analyzed. Growth for P. sanguineus was best in rice husk while corn stubble supported the highest growth rate for B. adusta. Among the substrates tested, cedar sawdust produced the highest cellulolytic activities in both fungal species, followed by oak sawdust and wheat straw. Xylanolytic activity was best in oak and cedar sawdust for both species. We found no correlation between growth and enzyme production. Zymogram analysis of xylanases and cellulases showed that growth in different substrates produced particular combinations of protein bands with hydrolytic activity.

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Acknowledgments

We are grateful to Jorge Martínez Herrera for providing Jatropha curcas seed husk. We are indebted with Chris Wood for his critical reading of this manuscript. This work was funded by CONACyT grant 48256Z and grant 13/2007 from UAM-Cuajimalpa. R.E. Q.-C. received a CONACyT scholarship (no. 47895) and N. P.-M. a CONACyT-FOMIX 93760 scholarship. CONACyT also provided a postdoctoral fellowship to C. M-A (Exp. no. 050272).

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Authors and Affiliations

  1. Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001 Col. Chamilpa, 62209, Cuernavaca, Morelos, México

    Rosa Estela Quiroz-Castañeda & Claudia Martínez-Anaya

  2. Laboratorio de Biología Molecular de Hongos, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001 Col. Chamilpa, 62209, Cuernavaca, Morelos, México

    Rosa Estela Quiroz-Castañeda & Jorge Folch-Mallol

  3. Facultad de Ciencias Biológicas, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001 Col. Chamilpa, 62209, Cuernavaca, Morelos, México

    Nancy Pérez-Mejía

  4. Centro de Investigaciones Biológicas, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001 Col. Chamilpa, 62209, Cuernavaca, Morelos, México

    Lourdes Acosta-Urdapilleta

Authors
  1. Rosa Estela Quiroz-Castañeda
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  2. Nancy Pérez-Mejía
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  3. Claudia Martínez-Anaya
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  4. Lourdes Acosta-Urdapilleta
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  5. Jorge Folch-Mallol
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Correspondence to Jorge Folch-Mallol.

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Rosa Estela Quiroz-Castañeda, Nancy Pérez-Mejía are the authors contributed equally to this work.

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Quiroz-Castañeda, R.E., Pérez-Mejía, N., Martínez-Anaya, C. et al. Evaluation of different lignocellulosic substrates for the production of cellulases and xylanases by the basidiomycete fungi Bjerkandera adusta and Pycnoporus sanguineus . Biodegradation 22, 565–572 (2011). https://doi.org/10.1007/s10532-010-9428-y

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  • DOI: https://doi.org/10.1007/s10532-010-9428-y

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