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Characterisation of low molecular weight glutenin subunit genes from Pseudoroegneria spicata and Pd. strigosa

  • Plant Genetics • Original Paper
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Abstract

We report the characterisation of nine novel low molecular weight glutenin subunit (LMW-GS) genes from two Pseudoroegneria species, Pd. spicata and Pd. strigosa. We found that all nine LMW-GS genes possess the same primary structure shared by other published LMW-GS. Five genes encode LMW-i type subunits, three encode LMW-m type subunits and one encodes a peptide similar to B-3 hordeins of Hordeum chilense. No LMW-s type subunit genes were found in Pseudoroegneria. One subunit, PSt24-LMW-2, contains six conserved cysteine residues, and the other eight subunits all contain eight cysteine residues. We show that one cysteine residue is located in the signal peptide of PSt24-LMW-1, suggesting a mature peptide containing only seven cysteine residues. Phylogenetic analysis indicates that the LMW-GS genes from the St genome cluster together and suggests a distant relationship with LMW-GS of the A and B genomes of wheat. Slippage/unequal crossing over and illegitimate recombination are effective mechanisms for enriching variations of seed storage proteins.

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Acknowledgements

This work was supported by funds from the Natural Science Foundation of China (nos. 31271707 and 31000568) and the Independent Innovation Foundation of Shandong University (2012TS009). We thank Dr. Austin Cape for the careful reading and feedback.

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Authors and Affiliations

  1. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People’s Republic of China

    Lumin Qin, Daozheng Yang, Guangmin Xia & Shuwei Liu

  2. Forest and Wetland Institute, Shandong Academy of Forestry, Jinan, 250014, People’s Republic of China

    Yu Liang

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  1. Lumin Qin
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  2. Yu Liang
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  3. Daozheng Yang
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  4. Guangmin Xia
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Correspondence to Shuwei Liu.

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Lumin Qin and Yu Liang contributed equally to this paper.

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Qin, L., Liang, Y., Yang, D. et al. Characterisation of low molecular weight glutenin subunit genes from Pseudoroegneria spicata and Pd. strigosa . J Appl Genetics 56, 27–35 (2015). https://doi.org/10.1007/s13353-014-0229-6

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  • DOI: https://doi.org/10.1007/s13353-014-0229-6

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