Abstract
In the present study, The hexaploid species Roegneria kamoji (StStYYHH genomes) in Triticeae produced a single high molecular weight glutenin subunit (HMW-GS) 1St1.2 in its endosperm. 1St1.2 encoded 404 amino acid, which is shorter than previous reported HMW-GSs in Triticeae. The N-terminal domains of 1St1.2 resemble that of the y-type subunits, while their C-terminal domains are similar to the x-type subunits. We discovered many variation types of polypeptides in central repetitive domain. There also have amino acid mutations in the N-terminal domain. The results of phylogenetic analysis suggested that 1St1.2 might be expression from the St genome. Therefore, the results indicated that 1St1.2 is a novel HMW-GS variant or isoform, and the characterization of the subunit can enhance our understanding on the structural differentiation and evolutionary relationships of HMW-GSs in wheat and its wild relatives.
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References
Branlard G., Autran J.C. & Monneveux P. 1989. High molecular weight glutenin subunit in durum wheat (T. durum). Theor. Appl. Genet. 78: 353–358.
Cainong J.C., Bockus W.W., Feng Y., Chen P., Qi L., Sehgal S.K., Danilova T.V., Koo D.H., Friebe B. & Gill B.S. 2015. Chromosome engineering, mapping, and transferring of resistance to Fusarium head blight disease from Elymus tsukushiensis into wheat. Theor. Appl. Genet. 128: 1019–1027.
Cui Z.G., Zhang H,Q., Li Q., Yang C.R., Huang J., Luo X.M. & Zhou Y.H. 2014. The production performance of 3 Roegneria kainoji in the Chengdu plain. Grassl. Sci. 31: 732–736. (In Chinese with English abstract)
Debustos A. & Jouve N. 2003. Characterization and analysis of new HMW-glutenin alleles encoded by the Glu-Rl locus of Secale cereale. Theor. Appl. Genet. 107: 74–83.
Doyle J.J. & Doyle J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11–15.
Field J.M., Tatham A.S. & Shewry P.R. 1987. The structure of a high-Mr subunit of durum wheat (Triticum, durum) gluten. Biochem. J. 247: 215–221.
Harberd N.P., Bartels D. & Thompson R.D. 1986. DNA restriction fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat. Biochem. Genet. 24: 579–596.
Jiang Q.T., Wei Y.M., Lu Z.X., Liu T., Wang J.R., Pu Z.E., Lan X.J. & Zheng Y.L. 2010. Characterization of a novel variant HMW-glutenin gene from Elymus canadensis. Gene & Genomics 32: 361–367.
Kuo P.C. 1987. Pooideae Flora Reipublicae Popularis Sinicae 9 (3). Science Press, Beijing, pp. 59–104.
Li F., Jiang X.L., Wei Y.F., Xia G.M. & Liu S.W. 2012. Characterization of a novel type of HMW subunit of glutenin from Australopyrum, retrofractum. Gene 492: 65–70.
Payne P.I. 1987. Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu. Rev. Plant Phys. 38: 141–153.
Payne P.I., Corfleld K.G. & Blackman J.A. 1981. Correlation between the inheritance of certain high-molecular-weight sub-units of glutenin and bread-making quality in progenies of six crosses of bread wheat. J. Sci. Food Agric. 32: 51–60.
Payne P.I., Holt L.M. & Worland A.J. 1982. Structural and ge-netical studies of high-molecular-weight subunits of wheat glutenin. Part 3. Telocentric mapping of the subunit genes on the long arm of the homoeologous group 1 chromosomes. Theor. Appl. Genet. 63: 129–138.
Ramu C., Sugawara H., Koike T., Lopez R., Gibson T.J., Higgins D.G. & Thompson J.D. 2003. Multiple sequence alignment with the Clustal series of programs. Nucl. Acids Res. 13: 3497–3500.
Sambrook J., Fritsch E.F. & Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.
Shewry P.R., Gilbert S.M., Savage A.W.J., Tatham A.S., Wan Y.F., Belton P.S., Wellner N., D’Ovidio R., Bekes F. & Hal-ford N.G. 2003a. Sequence and properties of HMW subunit 1BX20 from pasta wheat (Triticum durum) which is associated with poor end use properties. Theor. Appl. Genet. 106: 744–750.
Shewry P.R., Halford N.G. & Laflandra D. 2003b. Genetics of wheat gluten proteins. Adv. Genet. 49: 111–184.
Shewry P.R., Tatham A.S., Barro P. & Lazzeri P. 1995. Biotechnology of breadmaking: unraveling and manipulating the multi-protein gluten complex. Biotechnology 13: 1185–1190.
Sun X., Hu S., Liu X., Qian W., Hao S., Zhang A. & Wang D. 2006. Characterization of the HMW glutenin subunits from Aegilops searsii and identifi cation of a novel variant HMW glutenin subunit. Theor. Appl. Genet. 113: 631–641.
Sun Y.Q., Pu Z.J., Dai S.F., Pu X.X., Liu D.C., Wu B.H., Lan X.J., Wei Y.M., Zheng Y.L. & Yan Z.H. 2014. Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus. Dev. Genes Evol. 224: 57–64.
Tamura K., Peterson D., Peterson N., Stecher G., Nei M. & Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 2731–2739.
Tatham A.S., Drake A.F. & Shewry P.R. 1990. Conformational studies of synthetic peptides corresponding to the repetitive regions of the HMW-GS of wheat. J Cereal Sci. 11: 189–200.
Wan Y.F., Wang D.W., Shewry P.R. & Halford N.G. 2002. Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theor. Appl. Genet. 104: 828–839.
Wan Y.F., Yen C, Yang J.L. & Liu F.Q. 1997. Evaluation of Roegnerio, for resistance to head scab caused by Fusarium graminearum Schwabe. Genet. Resour. Crop Ev. 44: 211–215.
Wang J.R., Yan Z.H. & Wei Y.M. 2004. A novel highmolecular-weight glutenin subunit gene Eel.5 from Elytrigia elongate (Host) Nevski. J. Cereal Sci. 40: 289–294.
Wang S.B., Han H.N., Liang Y., Sun L., Xia G.M. & Liu S.W. 2014. Isolation and characterization of novel Glu-Stl alleles from Pseudoroegneria spicata and Pd. strigosa. Genetica 142: 433–440.
Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (Nos. 31470305, 31270243), Special Fund for Agro-Scientific Research in the Public Interest of China (Grant No. 201003021), and the Science and Technology Bureau and Education Bureau of Sichuan Province, China. The authors are highly thankful to Dr.Zehong Yan of Triticeae Research Institute, Sichuan Agricultural University for his kind helps on experiment discussions.
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Lei, YX., Yu, K., Wang, Y. et al. Molecular cloning of HMW glutenin subunits from Roegneria kamoji (Poaceae: Triticeae). Biologia 71, 414–420 (2016). https://doi.org/10.1515/biolog-2016-0055
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DOI: https://doi.org/10.1515/biolog-2016-0055