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Expression and clinical evidence of miR-494 and PTEN in non-small cell lung cancer

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Tumor Biology

Abstract

The aim of this study was to explore the expression and clinical significance of miR-494 and PTEN (phosphatase and tensin homologue deleted on chromosome ten) in non-small cell lung cancer (NSCLC). Immunohistochemistry for PTEN and in situ hybridization (ISH) for miR-494 were performed in 92 NSCLC tissues and 10 normal lung tissues to detect their expression, and correlation between their expression with clinical characteristics and prognosis was analyzed. The expression of miR-494 was significantly higher in NSCLC than in normal lung tissues (P = 0.004). The positive expression of PTEN protein in the lung carcinoma tissues was significantly lower than that in the normal lung tissues (P = 0.013), while the level of miR-494 expression was negatively correlated with PTEN expression (r = −0.577, P < 0.01). The high positive rate of miR-494 was positively correlated with pathological TNM (p-TNM) staging and lymph node metastasis. The expression of miR-494 was negatively correlated with grade of differentiation. However, the expression of PTEN was positively correlated with grade of differentiation. Patients with over-expression of miR-494 had a shorter overall survival (OS), while the negative group of PTEN was correlated with poor OS. MiR-494 over-expression and low PTEN expression are closely related to tumor p-TNM staging and lymph node metastasis, differentiation, and OS. Combined detection of PTEN and miR-494 can aid in determining malignancy degree and the prognosis of patients with NSCLC. MiR-494 may be served as a novel prognostic factor and may lead to new treatment strategies for NSCLC.

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    Authors and Affiliations

    1. Department of Medical Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, No.52, East Meihua Road, Zhuhai, Guangdong, China, 519000

      Juan Wang, Yifeng Liao, Nan Chen & Hongyu Zhang

    2. Department of Laboratory, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, China, 519000

      Hongtao Chen

    3. State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, China

      Tianze Liu

    4. Department of Nephrology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, China, 519000

      Huitao Zhang

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    1. Juan Wang
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    2. Hongtao Chen
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    3. Yifeng Liao
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    4. Nan Chen
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    5. Tianze Liu
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    7. Hongyu Zhang
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    Correspondence to Hongyu Zhang.

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    Juan Wang and Huitao Zhang contributed equally to this work.

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    Wang, J., Chen, H., Liao, Y. et al. Expression and clinical evidence of miR-494 and PTEN in non-small cell lung cancer. Tumor Biol. 36, 6965–6972 (2015). https://doi.org/10.1007/s13277-015-3416-0

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