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Laccase-mediator system produced by Trametes hirsuta Bm-2 on lignocellulosic substrate improves dye decolorization

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Abstract

Lignin is a source for obtaining natural phenols with high commercial value that can act as redox mediators enhancing effects in dye decolorization. In this study Trametes hirsuta Bm-2 was grown on wheat bran to produce laccases and phenol extracts (PE). Ultrafiltered phenols obtained at different times were evaluated in their potential as redox mediators of laccase activity and indigo carmin decolorization. Laccase activity (L) on ABTS increased up to 12.4 times with L/PE72 compared with laccase alone and L/PE48 showed the highest level of dye decolorization (97%) compared with laccase (12%). The chromatographic analysis by HPLC showed variation in the profile and concentration of phenols at different times of culture. Stability of the laccase mediator system (LMs) in dye decolorization was maintained over 3 months. Our results suggest the use of natural mediators as a strategy for improving efficiency in dye biodegradation by laccase-producing fungi.

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Acknowledgements

The authors wish to express their gratitude to National Science and Technology Council, Mexico, for providing the financial support for this research (Project No. 248295).

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Authors and Affiliations

  1. Depto. Ingeniería Química y Bioquímica, Instituto Tecnológico de Mérida, CP. 97118, Mérida, Yucatán, Mexico

    Wendy Ancona-Escalante, Luis Pool-Yam, Abraham Can-Cauich, Gabriel Lizama-Uc & Sara Solís-Pereira

  2. Unidad de Energía Renovable, Centro de Investigación Científica de Yucatán A.C., Carretera Sierra Papacal-Chuburna Puerto, km 5, Sierra Papacal, CP. 97302, Mérida, Yucatán, Mexico

    Raul Tapia-Tussell

Authors
  1. Wendy Ancona-Escalante
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  2. Raul Tapia-Tussell
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  3. Luis Pool-Yam
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  4. Abraham Can-Cauich
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  5. Gabriel Lizama-Uc
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  6. Sara Solís-Pereira
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Contributions

All the authors contributed to this work. Sara Solis-Pereira conceived, designed and wrote the paper; Ancona-Escalante performed the experiments and analyzed the data of laccase activity and phenol extract; Lizama-Uc, Pool-Yam and Can-Cahuich performed the experiments and analyzed the decolorization data and HPLC; and Raul Tapia-Tussell participated in the data analysis and writing of the paper.

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Correspondence to Sara Solís-Pereira.

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Ancona-Escalante, W., Tapia-Tussell, R., Pool-Yam, L. et al. Laccase-mediator system produced by Trametes hirsuta Bm-2 on lignocellulosic substrate improves dye decolorization. 3 Biotech 8, 298 (2018). https://doi.org/10.1007/s13205-018-1323-y

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