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Development of a Prebiotic Oligosaccharide Rich Functional Beverage from Sweet Sorghum Stalk Biomass

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Abstract

Sweet sorghum with sugar-rich stalks is considered a potential bioenergy crop. In this study, the sucrose in stalk juice was transformed into oligosaccharides by employing microbial and enzymatic methods. The maximum oligosaccharide yield of 10–14 g L−1 was obtained from 20 g L−1 sucrose in the sweet sorghum juice, representing conversion of about 82%–87% sucrose. The caloric value of the modified sweet sorghum juice was reduced by more than 50%. The probiotic bacteria exhibited a noteworthy growth profile in the modified sweet sorghum juice enriched with the non-digestible oligosaccharides. The antioxidative properties of the sweet sorghum stalk juice were not altered in the bioprocessed juice. The modified juice did not show any significant change in sensory properties after sucrose to oligosaccharide transformation. Furthermore, the oligosaccharides in the juice exhibited tolerance to heat exposure and, therefore, is feasible for food processing applications. The results suggested sweet sorghum as low-cost biomass for the development of plant-based functional products.

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References

  1. da Silva, M.J., Pastina, M.M., de Souza, V.F., Schaffert, R.E., Carneiro, P.C.S., Noda, R.W.: Phenotypic and molecular characterization of sweet sorghum accessions for bioenergy production. PLoS ONE 12(8), e0183504 (2017)

    Article  Google Scholar 

  2. Vinutha, K.S., Rayaprolu, L., Yadagiri, K., Umakanth, A.V., Patil, J.V., Rao, P.V.: Sweet sorghum research and development in India: status and prospects. Sugar Technol. 16(2), 133–143 (2014)

    Article  Google Scholar 

  3. Kawahigashi, H., Kasuga, S., Okuizumi, H., Hiradate, S., Yonemaru, J.I.: Evaluation of Brix and sugar content in stem juice from sorghum varieties. Grassl. Sci. 59, 1 (2013). https://doi.org/10.1111/grs.12006

    Article  Google Scholar 

  4. Fernandes, G., Braga, T.G., Fischer, J., Parrella, R.A.C., de Resende, M.M., Cardoso, V.L.: Evaluation of potential ethanol production and nutrients for four varieties of sweet sorghum during maturation. Renew. Energy 71, 518–524 (2014)

    Article  Google Scholar 

  5. Rolz, C., de León, R., de Montenegro, A.L.M., Cifuentes, R.: Ethanol from sweet sorghum in a year-round production cycle. Biomass Convers. Biores. 4, 341–350 (2014)

    Article  Google Scholar 

  6. Ekefre, D.E., Mahapatra, A.K., Latimore Jr., M., Bellmer, D.D., Jena, U., Whitehead, G.J., Williams, A.L.: Evaluation of three cultivars of sweet sorghum as feedstocks for ethanol production in the Southeast United States. Heliyon. 3(12), e00490 (2017)

    Article  Google Scholar 

  7. Mathur, S., Umakanth, A.V., Tonapi, V.A., Sharma, R., Sharma, M.K.: Sweet sorghum as biofuel feedstock: recent advances and available resources. Biotechnol. Biofuels 10, 146 (2017)

    Article  Google Scholar 

  8. Datta, M., Poshadri, A., Srinivasa Rao, P., Ravinder Reddy, C.H., Reddy, B.V.S.: Innovative use of Sweet sorghum juice in the beverage industry. Int. Food Res. J. 19(4), 1361–1366 (2012)

    Google Scholar 

  9. Vanamala, J.K., Massey, A.R., Pinnamaneni, S.R., Reddivari, L., Reardon, K.F.: Grain and sweet sorghum (Sorghum bicolor L. Moench) serves as a novel source of bioactive compounds for human health. Crit. Rev. Food Sci. Nutr. 58(17), 2867–2881 (2018)

    Article  Google Scholar 

  10. Wang, Y., Meng, H., Cai, D., Wang, B., Qin, P., Wang, Z., Tan, T.: Improvement of l-lactic acid productivity from sweet sorghum juice by repeated batch fermentation coupled with membrane separation. Bioresour. Technol. 211, 291–297 (2016)

    Article  Google Scholar 

  11. Liu, Q., Wang, S., Zhi, J.F., Ming, H., Teng, D.: Efficient production of lactic acid from sweet sorghum juice by a newly isolated Lactobacillus salivarius CGMCC 775. Indian J. Microbiol. 53(3), 332–336 (2013)

    Article  Google Scholar 

  12. Samad, A., Zang, J., Chen, D., Xiaowen, C., Tucker, M., Liang, Y.: Sweet sorghum bagasse and corn stover serving as substrates for producing sophorolipids. J. Ind. Microbiol. Biotechnol. 44(3), 353–362 (2017)

    Article  Google Scholar 

  13. Cai, D., Wang, Y., Chen, C., Qin, P., Miao, Q., Zhang, C., Li, P., Tan, T.: Acetone–butanol–ethanol from sweet sorghum juice by an immobilized fermentation-gas stripping integration process. Bioresour. Technol. 211, 704–710 (2016)

    Article  Google Scholar 

  14. Johnston, D.B., Nghiem, N.: Evaluation of sweet sorghum juice for the production of lysine using Corynebacterium glutamicum. Fermentation 4, 29 (2018)

    Article  Google Scholar 

  15. Sharma, M., Patel, S.N., Lata, K., Singh, U., Krishania, M., Sangwan, R.S., Singh, S.P.: A novel approach of integrated bioprocessing of cane molasses for production of prebiotic and functional bioproducts. Bioresour. Technol. 219, 311–318 (2016)

    Article  Google Scholar 

  16. De Angelis, R.C.: Fisiologia da nutrição: fundamentos para nutrição e desnutrição, São Paulo. EDART 1, 43–53 (1977)

    Google Scholar 

  17. Zanini, D.J., Silva, M.H., Aguiar-Oliveira, E., Mazalli, M.R., Kamimura, E.S., Maldonado, R.R.: Spectrophotometric analysis of vitamin C in different matrices utilizing potassium permanganate. Eur. Int. J. Sci. Technol. 7, 2304–9693 (2018)

    Google Scholar 

  18. Willis, O.O., Mouti, M.E., Sila, D.N., Mwasaru, M., Thiongo, G., Murage, H., Ojijo, N.O.: Physico-chemical properties and antioxidant potential of syrup prepared from ‘madhura’ sweet sorghum (sorghum bicolor L. Moench) cultivar grown at different locations in Kenya. Sugar Technol. 15(3), 263–270 (2013)

    Article  Google Scholar 

  19. Sharma, M., Sangwan, R.S., Khatkar, B.S., Singh, S.P.: Alginate–pectin co-encapsulation of dextransucrase and dextranase for oligosaccharide production from sucrose feedstocks. Bioprocess Biosyst. Eng. 42(10), 1681–1693 (2019)

    Article  Google Scholar 

  20. Sharma, M., Patel, S.N., Sangwan, R.S., Singh, S.P.: Biotransformation of banana pseudostem extract into a functional juice containing value added biomolecules of potential health benefits. Indian J. Exp. Biol. 55, 453–462 (2017)

    Google Scholar 

  21. Wang, X., Shao, C., Liu, L., Guo, X., Xu, Y., Lü, X.: Optimization, partial characterization and antioxidant activity of an exopolysaccharide from Lactobacillus plantarum KX041. Int. J. Biol. Macromol. 103, 1173–1184 (2017)

    Article  Google Scholar 

  22. Jia, F., Chawhuaymak, J., Riley, M.R., Zimmt, W., Ogden, K.L.: Efficient extraction method to collect sugar from sweet sorghum. J. Biol. Eng. 7, 1 (2013)

    Article  Google Scholar 

  23. Hector, S., Willard, K., Bauer, R., Mulako, I., Slabbert, E., Kossmann, J., George, G.M.: Diverse exopolysaccharide producing bacteria isolated from milled sugarcane: implications for cane spoilage and sucrose yield. PLoS ONE 10(12), e0145487 (2015)

    Article  Google Scholar 

  24. Bah, A., Albano, H., Barbosa, J.B., Fhoula, I., Gharbi, Y., Najjari, A., Boudabous, A., Teixeira, P., Ouzari, H.I.: Inhibitory effect of Lactobacillus plantarum FL75 and Leuconostoc mesenteroides FL14 against foodborne pathogens in artificially contaminated fermented tomato juices. Biomed. Res. Int. (2019). https://doi.org/10.1155/2019/6937837

    Article  Google Scholar 

  25. Alonso, S., Castro, M.C., Berdasco, M., de la Banda, I.G., Moreno-Ventas, X., de Rojas, A.H.: Isolation and partial characterization of lactic acid bacteria from the gut microbiota of marine fishes for potential application as probiotics in aquaculture. Probiotics Antimicrob. 11(2), 569–579 (2019)

    Article  Google Scholar 

  26. Lata, K., Sharma, M., Patel, S.N., Sangwan, R.S., Singh, S.P.: An integrated bio-process for production of functional biomolecules utilizing raw and by-products from dairy and sugarcane industries. Bioprocess Biosyst. Eng. 41(8), 1121–1131 (2018)

    Article  Google Scholar 

  27. Jadaun, J.S., Narnoliya, L.K., Agarwal, N., Singh, S.P.: Catalytic biosynthesis of levan and short-chain fructooligosaccharides from sucrose-containing feedstocks by employing the levansucrase from Leuconostoc mesenteroides MTCC10508. Int. J. Biol. Macromol. 127, 486–495 (2019)

    Article  Google Scholar 

  28. Rajvanshi, K., Nimbkar, N.: Sweet Sorghum R and D at the Nimbkar Agricultural Research Institute (NARI).  https://nariphaltan.org/sorghum.pdf (2001). Accessed 12 July 2020

  29. Yang, L., Zhou, N., Tian, Y.: Purification, characterization, and biocatalytic potential of a novel dextranase from Chaetomium globosum. Biotechnol. Lett. 40(9–10), 1407–1418 (2018)

    Article  Google Scholar 

  30. Miletic, N., Nastasovic, A., Loos, K.: Immobilization of biocatalysts for enzymatic polymerizations: possibilities, advantages, applications. Bioresour. Technol. 115, 126–135 (2012)

    Article  Google Scholar 

  31. Ölçer, Z., Tanriseven, A.: Co-immobilization of dextransucrase and dextranase in alginate. Process Biochem. 45(10), 1645–1651 (2010)

    Article  Google Scholar 

  32. Graebin, N.G., Schöffer, J.D.N., Andrades, D.D., Hertz, P.F., Ayub, M.A., Rodrigues, R.C.: Immobilization of glycoside hydrolase families GH1, GH13, and GH70: state of the art and perspectives. Molecules 21(8), 1074 (2016)

    Article  Google Scholar 

  33. Tingirikari, J.M.R., Gomes, W.F., Rodrigues, S.: Efficient production of prebiotic gluco-oligosaccharides in orange juice using immobilized and co-immobilized dextransucrase. Appl. Biochem. Biotechnol. 183(4), 1265–1281 (2017)

    Article  Google Scholar 

  34. Wichienchot, S., Prasertsan, P., Hongpattarakere, T., Gibson, G.R., Rastall, R.A.: In vitro fermentation of mixed linkage glucooligosaccharides produced by Gluconobacter oxydans NCIMB 4943 by the human colonic microflora. Curr. Issues Intest. Microbiol. 7(1), 7–12 (2006)

    Google Scholar 

  35. Hu, Y., Heyer, C.M.E., Wang, W., Zijlstra, R.T., Gänzle, M.G.: Digestibility of branched and linear α-gluco-oligosaccharides in vitro and in ileal-cannulated pigs. Food Res. Int. 127, 108726 (2020). https://doi.org/10.1016/j.foodres.2019.108726

    Article  Google Scholar 

  36. Kothari, D., Goyal, A.: Gentio-oligosaccharides from Leuconostoc mesenteroides NRRL B-1426 dextransucrase as prebiotics and as a supplement for functional foods with anti-cancer properties. Food Funct. (2014). https://doi.org/10.1039/c4fo00802b

    Article  Google Scholar 

  37. Erdurmus, C., Yucel, C., Cınar, O., Yegin, A.B., Oten, M.: Bioethanol and sugar yields of sweet sorghum. Int. J. Eng. Sci. Technol. 7(11), 21–26 (2018). https://doi.org/10.9790/1813-0711022126

    Article  Google Scholar 

  38. Nguyen, T.T.H., Cho, J.Y., Seo, Y.S., Woo, H.J., Kim, H.K., Kim, G.J., Jhon, D.Y., Kim, D.: Production of a low calorie mandarin juice by enzymatic conversion of constituent sugars to oligosaccharides and prevention of insoluble glucan formation. Biotechnol. Lett. 37, 711–716 (2015)

    Article  Google Scholar 

  39. Kartawiria, I.S., Syamsu, K., Noor, E., Said, E.G.: Sorghum stalk juice pre-treatment method for bioethanol fermentation process. Energy Procedia 65, 140–145 (2015)

    Article  Google Scholar 

  40. Lin, D., et al.: An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules 21, 1374 (2016)

    Article  Google Scholar 

  41. Hou, Y., Ding, X., Hou, W.: Composition and antioxidant activity of water-soluble oligosaccharides from Hericium erinaceus. Mol. Med. Rep. 11, 3794–3799 (2015). https://doi.org/10.3892/mmr.2014.3121

    Article  Google Scholar 

  42. Van den Ende, W., Valluru, R.: Sucrose, sucrosyl oligosaccharides, and oxidative stress: scavenging and salvaging. J. Exp. Bot. 60(1), 9–18 (2009). https://doi.org/10.1093/jxb/ern297

    Article  Google Scholar 

  43. Hong, X., Wang, J.: Application of e-nose and e-tongue to measure the freshness of cherry tomatoes squeezed for juice consumption. Anal. Methods 6, 3133–3138 (2014)

    Article  Google Scholar 

  44. Ibrahim, O.O.: Functional oligosaccharides: chemicals structure, manufacturing, health benefits, applications and regulations. J. Food. Chem. Nanotechnol. 4(4), 65–76 (2018)

    Article  Google Scholar 

  45. Duar, R.M., Ang, P.T., Hoffman, M., Wehling, R., Hutkins, R., Schlegel, V.: Processing effects on four prebiotic carbohydrates supplemented in an extruded cereal and a low pH drink. Cogent Food Agric. 1, 1013782 (2015). https://doi.org/10.1080/23311932.2015.1013782

    Article  Google Scholar 

  46. López-Sanz, S., Montilla, A., Moreno, F.J., Villamiel, M.: Stability of oligosaccharides derived from lactulose during the processing of milk and apple juice. Food Chem. 183, 64–71 (2015). https://doi.org/10.1016/j.foodchem.2015.03.020

    Article  Google Scholar 

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Acknowledgements

Authors acknowledge the Department of Biotechnology (DBT), Government of India, for financial support. SPS cordially acknowledges the DBT Project-Grant, BT/PR17586/PFN/20/1195. MS acknowledges the Council of Scientific & Industrial Research (CSIR) for SRF fellowship, and the Department of Food Technology, Guru Jambheshwar University of Science and Technology, Hisar, for Ph.D. registration.

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Authors and Affiliations

  1. Center of Innovative and Applied Bioprocessing, S.A.S. Nagar, Sector-81 (Knowledge City), Mohali, 140 306, India

    Manisha Sharma, Rajender S. Sangwan & Sudhir P. Singh

  2. Department of Food Technology, Guru Jambheshwar University of Science and Technology, Hisar, 125 001, India

    Manisha Sharma & Bhupender S. Khatkar

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  1. Manisha Sharma
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Sharma, M., Sangwan, R.S., Khatkar, B.S. et al. Development of a Prebiotic Oligosaccharide Rich Functional Beverage from Sweet Sorghum Stalk Biomass. Waste Biomass Valor 12, 2001–2012 (2021). https://doi.org/10.1007/s12649-020-01156-5

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