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A Novel High-Molecular-Mass Bacteriocin Produced by Enterococcus faecium: Biochemical Features and Mode of Action

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Abstract

Discovery of a novel bacteriocin is always an event in sciences, since cultivation of most bacterial species is a general problem in microbiology. This statement is reflected by the fact that number of bacteriocins is smaller for tenfold comparing to known antimicrobial peptides. We cultivated Enterococcus faecium on simplified medium to reduce amount of purification steps. This approach allows to purify the novel heavy weight bacteriocin produced by E. faecium ICIS 7. The novelty of this bacteriocin, named enterocin-7, was confirmed by N-terminal sequencing and by comparing the structural-functional properties with available data. Purified enterocin-7 is characterized by a sequence of amino acid residues having no homology in UniProt/SwissProt/TrEMBL databases: NH2 - Asp - Ala - His - Leu - Ser - Glu - Val - Ala - Glu - Arg - Phe - Glu - Asp - Leu - Gly. Isolated thermostable protein has a molecular mass of 65 kDa, which allows it to be classified into class III in bacteriocin classification schemes. Enterocin-7 displayed a broad spectrum of activity against some Gram-positive and Gram-negative microorganisms. Fluorescent microscopy and spectroscopy showed the permeabilizing mechanism of the action of enterocin-7, which is realized within a few minutes.

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Funding

This work was partially supported by the Program of fundamental research of Ural branch of RAS, project No. 15-4-4-28 “Analysis of the resistom, its phenotypic manifestations and QS-systems of bacterial populations in order to develop a new methods for overcoming the antibiotic resistance of microorganisms.”

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Authors and Affiliations

  1. Tyumen State University, Volodarsky st, 6, Tyumen, Russian Federation, 625003

    A. S. Vasilchenko & A.V. Vasilchenko

  2. Institute of Cellular and Intracellular Symbiosis, RAS, Pionerskaya st, 11, Orenburg, Russian Federation, 460000

    A.V. Valyshev

  3. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya st, 16/10, Moscow, Russian Federation, 117997

    E.A. Rogozhin

  4. Gause Institute of New Antibiotics, Bolshaya Pirogovskaya st, 11, Moscow, Russian Federation, 119021

    E.A. Rogozhin

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  1. A. S. Vasilchenko
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  2. A.V. Vasilchenko
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  3. A.V. Valyshev
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  4. E.A. Rogozhin
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Correspondence to A. S. Vasilchenko.

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Vasilchenko, A.S., Vasilchenko, A., Valyshev, A. et al. A Novel High-Molecular-Mass Bacteriocin Produced by Enterococcus faecium: Biochemical Features and Mode of Action. Probiotics & Antimicro. Prot. 10, 427–434 (2018). https://doi.org/10.1007/s12602-018-9392-0

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