Abstract
Antibiotic resistance is a growing concern that is affecting public health globally. The search for alternative antimicrobial agents has become increasingly important. Antimicrobial peptides (AMPs) produced by Bacillus spp. have emerged as a promising alternative to antibiotics, due to their broad-spectrum antimicrobial activity against resistant pathogens. In this review, we provide an overview of Bacillus-derived AMPs, including their classification into ribosomal (bacteriocins) and non-ribosomal peptides (lipopeptides and polyketides). Additionally, we delve into the molecular mechanisms of AMP production and describe the key biosynthetic gene clusters involved. Despite their potential, the low yield of AMPs produced under normal laboratory conditions remains a challenge to large-scale production. This review thus concludes with a comprehensive summary of recent studies aimed at enhancing the productivity of Bacillus-derived AMPs. In addition to medium optimization and genetic manipulation, various molecular strategies have been explored to increase the production of recombinant antimicrobial peptides (AMPs). These include the selection of appropriate expression systems, the engineering of expression promoters, and metabolic engineering. Bacillus-derived AMPs offer great potential as alternative antimicrobial agents, and this review provides valuable insights on the strategies to enhance their production yield, which may have significant implications for combating antibiotic resistance.
Key points
• Bacillus-derived AMP is a potential alternative therapy for resistant pathogens
• Bacillus produces two main classes of AMPs: ribosomal and non-ribosomal peptides
• AMP yield can be enhanced using culture optimization and molecular approaches
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(Modified from Caulier et al., 2019)
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References
Ahire JJ, Kashikar MS, Lakshmi SG, Madempudi R (2020) Identification and characterization of antimicrobial peptide produced by indigenously isolated Bacillus paralicheniformis UBBLi30 strain. 3 Biotech 10:112. https://doi.org/10.1007/s13205-020-2109-6
Al-Thubiani ASA, Maher YA, Fathi A, Abourehab MAS, Alarjah M, Khan MSA, Al-Ghamdi SB (2018) Identification and characterization of a novel antimicrobial peptide compound produced by Bacillus megaterium strain isolated from oral microflora. Saudi Pharm J 26:1089–1097. https://doi.org/10.1016/j.jsps.2018.05.019
Aleti G, Sessitsc A, Brader G (2015) Genome mining: prediction of lipopeptides and polyketides from Bacillus and related Firmicutes. Comput Struct Biotechnol J 13:192–203. https://doi.org/10.1016/j.csbj.2015.03.003
Annunziato G, Costantino G (2020) Antimicrobial peptides (AMPs): a patent review (2015–2020). Expert Opin Ther Pat. https://doi.org/10.1080/13543776.2020.1851679
Ansari A, Zohra RR, Tarar OM, Qader SAU, Aman A (2018) Screening, purification and characterization of thermostable, protease resistant Bacteriocin active against methicillin resistant Staphylococcus aureus (MRSA). BMC Microbiol 18(1):1–10. https://doi.org/10.1186/s12866-018-1337-y
Arias AA, Ongena M, Devreese B, Terrak M, Joris B, Fickers P (2013) Characterization of amylolysin, a novel lantibiotic from Bacillus amyloliquefaciens GA1. PLoS ONE 8(12):1–10. https://doi.org/10.1371/journal.pone.0083037
Arima K, Kakinuma A, Tamura G (1968) Surfactin, a crystalline peptidelipid surfactant produced by Bacillus subtilis: Isolation, characterization and its inhibition of fibrin clot formation. Biochem Biophys Res Commun 31(3):488–494. https://doi.org/10.1016/0006-291X(68)90503-2
Aslam B, Wang W, Arshad MI, Khurshid M, Muzammil S, Rasool MH, Nisar MA, Alvi RF, Aslam MA, Qamar MU, Salamat MKF, Baloch Z (2018) Antibiotic resistance : a rundown of a global crisis. Infect Drug Resist 11:1645–1658 (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6188119/)
Ayed HB, Maalej H, Hmidet N, Nasri M (2015) Isolation and biochemical characterisation of a bacteriocin-like substance produced by Bacillus amyloliquefaciens An6. J Glob Antimicrob Resist 3(4):255–261. https://doi.org/10.1016/j.jgar.2015.07.001
Baharudin MMAA, Ngalimat MS, Shariff FM, Yusof ZNB, Karim M, Baharum SN, Sabri S (2021) Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus. PLoS ONE 16(5):1–20. https://doi.org/10.1371/journal.pone.0251514
Barale SS, Ghane SG, Sonawane KD (2022) Purification and characterization of antibacterial surfactin isoforms produced by Bacillus velezensis SK. AMB Express 12(7):7. https://doi.org/10.1186/s13568-022-01348-3
Barbosa J, Caetano T, Mendo S (2015) Class I and class II lanthipeptides produced by Bacillus spp. J Nat Prod 78(11):2850–2866. https://doi.org/10.1021/np500424y
Butkhot N, Soodsawaeng P, Samutsan S, Chotmongcol K, Vuthiphandchai V, Nimrat S (2019) New perspectives for surveying and improving Thai dried seafood qualities using antimicrobials produced by Bacillus velezensis BUU004 against foodborne pathogens. Sci Asia 45:116–126. https://doi.org/10.2306/scienceasia1513-1874.2019.45.116
Caetano T, Krawczyk JM, Mösker E, Süssmuth RD, Mendo S (2011) Heterologous expression, biosynthesis, and mutagenesis of type II lantibiotics from Bacillus licheniformis in Escherichia coli. Chem Biol 18:90–100. https://doi.org/10.1016/j.chembiol.2010.11.010
Cai D, Zhang B, Zhu J, Xu H, Liu P, Wang Z, Li J, Yang Z, Ma X, Chen S (2020) Enhanced bacitracin production by systematically engineering S-Adenosylmethionine supply modules in Bacillus licheniformis. Front Bioeng Biotechnol 8:305. https://doi.org/10.3389/fbioe.2020.00305
Cai D, Zhu J, Zhu S, Lu Y, Zhang B, Lu K, Li J, Ma X, Chen S (2019) Metabolic engineering of main transcription factors in carbon, nitrogen, and phosphorus metabolisms for enhanced production of bacitracin in Bacillus licheniformis. ACS Synth Biol 8:866–875. https://doi.org/10.1021/acssynbio.9b00005
Caulier S, Nannan C, Gillis A, Licciardi F, Bragard C, Mahillon J (2019) Overview of the antimicrobial compounds produced by members of the Bacillus subtilis group. Front Microbiol 10:302. https://doi.org/10.3389/fmicb.2019.00302
Chalasani AG, Dhanarajan G, Nema S, Sen R, Roy U (2015) An antimicrobial metabolite from Bacillus sp.: significant activity against pathogenic bacteria including multidrug-resistant clinical strains. Front Microbiol 6(1335):1–10. https://doi.org/10.3389/fmicb.2015.01335
Chen CH, Lu TK (2020) Development and challenges of antimicrobial peptides for therapeutic applications. Antibiot 9:24. https://doi.org/10.3390/antibiotics9010024
Chen L, Chong X, Zhang YY, Lv Y, Hu YS (2020) Genome shuffling of Bacillus velezensis for enhanced surfactin production and variation analysis. Curr Microbiol 77:71–78. https://doi.org/10.1007/s00284-019-01807-4
Chen L, Heng J, Qin S, Bian K (2018) A comprehensive understanding of the biocontrol potential of Bacillus velezensis LM2303 against Fusarium head blight. PLoS ONE 13(6):1–22. https://doi.org/10.1371/journal.pone.0198560
Chen XH, Koumoutsi A, Scholz R, Borriss R (2009) More than anticipated - production of antibiotics and other secondary metabolites by Bacillus amyloliquefaciens FZB42. J Mol Microbiol Biotechnol 16:14–24. https://doi.org/10.1159/000142891
Ciandrini E, Morroni G, Arzeni D, Kamysz W, Neubauer D, Kamysz E, Cirioni O, Brescini L, Baffone W, Campana R (2018) Antimicrobial activity of different antimicrobial peptides (AMPs) against clinical methicillin-resistant Staphylococcus aureus (MRSA). Curr Top Med Chem 18:1–9. https://doi.org/10.2174/1568026618666181022140348
Dang Y, Zhao F, Liu X, Fan X, Huang R, Gao W, Wang S, Yang C (2019) Enhanced production of antifungal lipopeptide iturin A by Bacillus amyloliquefaciens LL3 through metabolic engineering and culture conditions optimization. Microb Cell Fact 18(68):1–14. https://doi.org/10.1186/s12934-019-1121-1
Deng T, Ge H, He H, Liu Y, Zhai C, Feng L, Yi L (2017) The heterologous expression strategies of antimicrobial peptides in microbial systems. Prot Expr Purif 140:52–59. https://doi.org/10.1016/j.pep.2017.08.003
Desmyttere H, Deweer C, Muchembled J, Sahmer K, Jacquin J, Coutte F, Jacques P (2019) Antifungal activities of Bacillus subtilis lipopeptides to two Venturia inaequalis strains possessing different tebuconazole sensitivity. Front Microbiol 10:2327. https://doi.org/10.3389/fmicb.2019.02327
Dhali D, Coutte F, Arias AA, Auger S, Bidnenko V, Chataigné G, Lalk M, Niehren J, Sousa JD, Versari C, Jacques P (2017) Genetic engineering of the branched fatty acid metabolic pathway of Bacillus subtilis for the overproduction of surfactin C14 isoform. Biotechnol J 12(7):1600574. https://doi.org/10.1002/biot.201600574
Diabankana RGC, Shulga EU, Validov SZ, Afordoanyi DM (2022) Genetic characteristics and enzymatic activities of Bacillus velezensis KS04AU as a stable biocontrol agent against phytopathogens. Int J Plant Biol 13:201–222. https://doi.org/10.3390/ijpb13030018
Dimkic I, Stankovic S, Nišavic M, Petkovic M, Ristivojevic P, Fira D, Beric T (2017) The profile and antimicrobial activity of Bacillus lipopeptide extracts of five potential biocontrol strains. Front Microbiol 8:925. https://doi.org/10.3389/fmicb.2017.00925
Duan Y, Chen R, Zhang R, Jiang W, Chen X, Yin C, Mao Z (2021) Isolation, identification, and antibacterial mechanisms of Bacillus amyloliquefaciens QSB-6 and its effect on plant roots. Front Microbiol 12:746799. https://doi.org/10.3389/fmicb.2021.746799
Dulyayangkul P, Wan Nur Ismah WAK, Douglas EJA, Avison MB (2020) Mutation of kvrA causes OmpK35 and OmpK36 porin downregulation and reduced meropenem-vaborbactam susceptibility in KPC-producing Klebsiella pneumoniae. ASM J 64(7):e02208-19. https://doi.org/10.1128/AAC.02208-19
Dunlap CA, Bowman MJ, Rooney AP (2019) Iturinic lipopeptide diversity in the Bacillus subtilis species group – important antifungals for plant disease biocontrol applications. Front Microbiol 10:1–12. https://doi.org/10.3389/fmicb.2019.01794
Ebrahimi R, Pournejati R, Karbalaei-Heidari HR (2021) Pseudomonas aeruginosa growth inhibitor, PAGI264: a natural product from a newly isolated marine bacterium, Bacillus sp. strain REB264. Iran J Sci Technol Trans A Sci 45:1165–1175. https://doi.org/10.1007/s40995-021-01107-2
Egorov AM, Ulyashova MM, Rubtsova MY (2018) Bacterial enzymes and antibiotic resistance. Acta Nat 10(4):33–48
Ghanbarzadeh Z, Hemmati S, Mohagheghzadeh A (2022) Humanizing plant-derived snakins and their encrypted antimicrobial peptides. Biochim 199:92–111. https://doi.org/10.1016/j.biochi.2022.04.011
Gao L, She M, Shi J, Cai D, Wang D, Xiong M, Shen G, Gao J, Zhang M, Yang Z, Chen S (2022) Enhanced production of iturin A by strengthening fatty acid synthesis modules in Bacillus amyloliquefaciens. Front Bioeng Biotechnol. https://doi.org/10.3389/fbioe.2022.974460
Gao XY, Liu Y, Miao LL, Li EW, Sun GX, Liu Y, Liu ZP (2017) Characterization and mechanism of anti-Aeromonas salmonicida activity of a marine probiotic strain, Bacillus velezensis V4. Appl Microbiol Biotechnol 101:3759–3768. https://doi.org/10.1007/s00253-017-8095-x
Gómez-Sequeda N, Ruiz J, Ortiz C, Urquiza M, Torres R (2020) Potent and specific antibacterial activity against Escherichia coli O157:H7 and methicillin resistant Staphylococcus aureus (MRSA) of G17 and G19 peptides encapsulated into poly-lactic-co-glycolic acid (PLGA) nanoparticles. Antibiot 9(7):384. https://doi.org/10.3390/antibiotics9070384
Gowrishankar S, Kamaladevi A, Ayyanar KS, Balamurugan K, Pandian SK (2015) Bacillus amyloliquefaciens-secreted cyclic dipeptide- cyclo(L-Leucyl- L-Prolyl) inhibits biofilm and virulence in methicillin-resistant Staphylococcus aureus. RSC Adv 5(116):95788–95804. https://doi.org/10.1039/C5RA11641D
Grady EN, MacDonald J, Ho MT, Weselowski B, McDowell T, Solomon O, Renaud J, Yuan ZC (2019) Characterization and complete genome analysis of the surfactin-producing, plant-protecting bacterium Bacillus velezensis 9D–6. BMC Microbiol 19(5):1–14. https://doi.org/10.1186/s12866-018-1380-8
Guez J, Coucheney F, Guy J, Béchet M, Fontanille P, Chihib N, Niehren J, Coutte F, Jacques P (2022) Bioinformatics modelling and metabolic engineering of the branched chain amino acid pathway for specific production of mycosubtilin isoforms in Bacillus subtilis. Metab 12:107. https://doi.org/10.3390/metabo12020107
Halami PM (2019) Sublichenin, a new subtilin-like lantibiotics of probiotic bacterium Bacillus licheniformis MCC 2512 T with antibacterial activity. Microb Pathog 128:139–146. https://doi.org/10.1016/j.micpath.2018.12.044
Han FF, Liu YF, Xie YG, Gao YH, Luan C, Wang YZ (2011) Antimicrobial peptides derived from different animals: comparative studies of antimicrobial properties, cytotoxicity and mechanism of action. World J Microbiol Biotechnol 27:1847–1857. https://doi.org/10.1007/s11274-010-0643-9
Harwood CR, Mouillon JM, Pohl S, Arnau J (2018) Secondary metabolite production and the safety of industrially important members of the Bacillus subtilis group. FEMS Microbiol Rev 42(6):721–738. https://doi.org/10.1093/femsre/fuy028
He M, Wen J, Yin Y, Wang P (2021) Metabolic engineering of Bacillus subtilis based on genome-scale metabolic model to promote fengycin production. 3 Biotech 11:448. https://doi.org/10.1007/s13205-021-02990-7
Herzner AM, Dischinger J, Szekat C, Josten M, Schmitz S, Yakeleba A, Reinartz R, Jansen A, Sahl HG, Piel J, Bierbaum G (2011) Expression of the lantibiotic mersacidin in Bacillus amyloliquefaciens FZB42. PLoS ONE 6(7):e22389. https://doi.org/10.1371/journal.pone.0022389
Hospet R, Thangadurai D, Cruz-Martins N, Sangeetha J, Anu Appaiah KA, Chowdhury ZZ, Bedi N, Soytong K, Al Tawahaj ARM, Jabeen S, Tallur MM (2021) Genome shuffling for phenotypic improvement of industrial strains through recursive protoplast fusion technology. Crit Rev Food Sci Nutr. https://doi.org/10.1080/10408398.2021.1983763
Hu F, Cai W, Lin J, Wang W, Li S (2021) Genetic engineering of the precursor supply pathway for the overproduction of the nC14-surfactin isoform with promising MEOR applications. Microb Cell Fact 20:96. https://doi.org/10.1186/s12934-021-01585-4
Hu HQ, Li XS, He H (2010) Characterization of an antimicrobial material from a newly isolated Bacillus amyloliquefaciens from mangrove for biocontrol of Capsicum bacterial wilt. Biol Control 54:359–365. https://doi.org/10.1016/j.biocontrol.2010.06.015
Hu Y, Nan F, Maina SW, Guo J, Wu S, Xin Z (2018) Clone of plipastatin biosynthetic gene cluster by transformation-associated recombination technique and high efficient expression in model organism Bacillus subtilis. J Biotechnol 288:1–8. https://doi.org/10.1016/j.jbiotec.2018.10.006
Hussein W (2019) Fengycin or plipastatin? A Confusing Question in Bacilli. Biotechnol 100(1):47–55. https://doi.org/10.5114/bta.2019.83211
Im SM, Yu NH, Joen HW, Kim SO, Park HW, Park AR, Kim JC (2020) Biological control of tomato bacterial wilt by oxydifficidin and difficidin-producing Bacillus methylotrophicus DR-08. Pestic Biochem Physiol 163:130–137. https://doi.org/10.1016/j.pestbp.2019.11.007
Iqbal S, Qasim M, Begum F, Rahman H, Sajid I (2018) Screening, characterization and optimization of antibacterial peptides, produced by Bacillus safensis strain MK-12 isolated from waste dump soil KP, Pakistan. BioRxiv. doi:https://doi.org/10.1101/308205
Janek T, Gudiña EJ, Połomska X, Biniarz P, Jama D, Rodrigues LR, Rymowicz W, Lazar Z (2021) Sustainable surfactin production by Bacillus subtilis using crude glycerol from different wastes. Molecules 26:3488. https://doi.org/10.3390/molecules26123488
Jawan R, Abbasiliasi S, Tan JS, Mustafa S, Halim M, Ariff AB (2020) Influence of culture conditions and medium compositions on the production of bacteriocin-like inhibitory substances by Lactococcus lactis GH1. Microorganisms 8(1454):1–14. https://doi.org/10.3390/microorganisms8101454
Jin X, Yao J, Fan H, Che Y, Pan J, Zhang L, Pan X, Gelbič I, Huang T, Guan X (2019) Heterologous expression and purification of BtCspB, a novel cold-shock protein-like bacteriocin from Bacillus thuringiensis BRC-ZYR2. World J Microbiol Biotechnol 35(2): 23. doi:https://doi.org/10.1007/s11274-019-2595-z
Jung J, Yu KO, Ramzi AB, Choe SH, Kim SW, Han SO (2012) Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC. Biotechnol Bioeng 109(9):2349–2356. https://doi.org/10.1002/bit.24524
Kaewklom S, Lumlert S, Kraikul W, Aunpad R (2013) Control of Listeria monocytogenes on sliced bologna sausage using a novel bacteriocin, amysin, produced by Bacillus amyloliquefaciens isolated from Thai shrimp paste (Kapi). Food Control 32(2):552–557. https://doi.org/10.1016/j.foodcont.2013.01.012
Kapoor G, Saigal S, Elongavan A (2017) Action and resistance mechanisms of antibiotics: a guide for clinicians. J Anaesthesiol Clin Pharmacol 33(3):300–305. https://doi.org/10.4103/joacp.JOACP_349_15
Kayalvizhi N, Rameshkumar N, Gunasekaran P (2016) Cloning and characterization of mersacidin like bacteriocin from Bacillus licheniformis MKU3 in Escherichia coli. J Food Sci Technol 53(5):2298–2306. https://doi.org/10.1007/s13197-016-2195-y
Kim YT, Kim SE, Lee WJ, Fumei Z, Cho MS, Moon JS, Oh HW, Park HY, Kim SU (2020) Isolation and characterization of a high iturin yielding Bacillus velezensis UV mutant with improved antifungal activity. PLoS ONE 15(12):e0234177. https://doi.org/10.1371/JOURNAL.PONE.0234177
Koo HB, Seo J (2019) Antimicrobial peptides under clinical investigation. Pept Sci 111(5):e24122. https://doi.org/10.1002/pep2.24122
Kosikowska P, Lesner A (2016) Antimicrobial peptides (AMPs) as drug candidates: a patent review (2003–2015). Expert Opin Ther Pat 26(6):689–702. https://doi.org/10.1080/13543776.2016.1176149
Koumoutsi A, Chen XH, Henne A, Liesegang H, Hitzeroth G, Franke P, Vater J, Borriss R (2004) Structural and functional characterization of gene clusters directing nonribosomal synthesis of bioactive cyclic lipopeptides in Bacillus amyloliquefaciens strain FZB42. J Bacteriol 186(4):1084–1096. https://doi.org/10.1128/JB.186.4.1084-1096.2004
Kourmentza K, Gromada X, Michael N, Degraeve C, Vanier G, Ravallec R, Coutte F, Karatzas KA, Jaureg P (2021) Antimicrobial activity of lipopeptide biosurfactants against foodborne pathogen and food spoilage microorganisms and their cytotoxicity. Front Microbiol 11:561060. https://doi.org/10.3389/fmicb.2020.561060
Kurata A, Yamaguchi T, Kira M, Kishimoto N (2019) Characterization and heterologous expression of an antimicrobial peptide from Bacillus amyloliquefaciens CMW1. Biotechnol Biotechnol Equip 33(1):886–893. https://doi.org/10.1080/13102818.2019.1627246
Kuroda K, Okumura K, Isogai H, Isogai E (2015) The human cathelicidin antimicrobial peptide LL-37 and mimics are potential anticancer drugs. Front Oncol 5:144. https://doi.org/10.3389/fonc.2015.00144
Kwon MJ, Steiniger C, Cairns TC, Wisecaver JH, Lind AL, Pohl C, Regner C, Rokas A, Meyer V (2021) Beyond the biosynthetic gene cluster paradigm: genome-wide coexpression networks connect clustered and unclustered transcription factors to secondary metabolic pathways. Microbiol Spectr 9(2):e0089821. https://doi.org/10.1128/spectrum.00898-21
Leclère V, Béchet M, Adam A, Guez JS, Wathelet B, Ongena M, Thonart P, Gancel F, Chollet-Imbert M, Jacques P (2005) Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism’s antagonistic and biocontrol activities. Appl Environ Microbiol 71(8):4577–4584. https://doi.org/10.1128/AEM.71.8.4577-4584.2005
Lee J, Hao Y, Blair PM, Melby JO, Agarwal V, Burkhart BJ, Nair SK, Mitchell DA (2013) Structural and functional insight into an unexpectedly selective N-methyltransferase involved in plantazolicin biosynthesis. Proc Natl Acad Sci U S A 110(32):12954–12959. https://doi.org/10.1073/pnas.1306101110
Lei J, Sun LC, Huang S, Zhu C, Li P, He J, Mackey V, Coy DH, He QY (2019) The antimicrobial peptides and their potential clinical applications. Am J Transl Res 11(7):3919–3931 (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6684887/)
Leja K, Myszka K, Czaczyk K (2011) Genome shuffling: a method to improve biotechnological processes. Biotechnol 92(4):345–351. https://doi.org/10.5114/bta.2011.46551
Li C, Blencke HM, Paulsen V, Haug T, Stensvåg K (2010) Powerful workhorses for antimicrobial peptide expression and characterization. Bioeng Bugs 1(3):217–220. https://doi.org/10.4161/bbug.1.3.11721
Li XY, Mao ZC, Wang YH, Wu YX, He YQ, Long CL (2012) ESI LC-MS and MS/MS characterization of antifungal cyclic lipopeptides produced by Bacillus subtilis XF-1. J Mol Microbiol Biotechnol 22(2):83–93. https://doi.org/10.1159/000338530
Li X, Yang H, Zhang D, Li X, Yu H, Shen Z (2015) Overexpression of specific proton motive force-dependent transporters facilitate the export of surfactin in Bacillus subtilis. J Ind Microbiol Biotechnol 42:93–103. https://doi.org/10.1007/s10295-014-1527-z
Li Z, Cheng Q, Guo H, Zhang R, Si D (2020) Expression of hybrid peptide ef-1 in Pichia pastoris, its purification, and antimicrobial characterization. Mol 25:5538. https://doi.org/10.3390/molecules25235538
Liang L, Fu Y, Deng S, Wu Y, Gao M (2022) Genomic, antimicrobial, and aphicidal traits of Bacillus velezensis ATR2, and its biocontrol potential against ginger rhizome rot disease caused by Bacillus pumilus. Microorganisms 10:63. https://doi.org/10.3390/microorganisms10010063
Liang X, Long Z, Wang X, Yang L, Lu B, Gao J (2020) An LCI-like protein APC2 protects ginseng root from Fusarium solani infection. J Appl Microbiol 130(1):165–178. https://doi.org/10.1111/jam.14771
Lihong D, Qinggang G, Peipei W, Shezeng L, Xiuyun L, Xiaoyun Z, Weisong Z, Ping M (2018) The effect of PhoR/PhoP two-component regulatory system on surfactin production in Bacillus subtilis NCD-2. Acta Phytopathol Sin 48(1):119–127. https://doi.org/10.13926/j.cnki.apps.000078
Lin R, Zhang Q, Yin L, Zhang Y, Yang Q, Liu K, Wang Y, Han S, Zhao H, Zhao H (2022) Isolation and characterization of a mycosubtilin homologue antagonizing Verticillium dahliae produced by Bacillus subtilis. PLoS ONE 17(6):e0269861. https://doi.org/10.1371/journal.pone.0269861
Liu D, Rubin GM, Chen DM, Ding Y (2021) Biocatalytic synthesis of peptidic natural products and related analogues. ISci 24(5):102512. https://doi.org/10.1016/j.isci.2021.102512
Lu H, Xu H, Yang P, Bilal M, Zhu S, Zhong M, Zhao L, Gu C, Liu S, Zhao Y, Geng C (2022) Transcriptome analysis of Bacillus amyloliquefaciens reveals fructose addition effects on fengycin synthesis. Genes 13(6):984. https://doi.org/10.3390/genes13060984
Lu JY, Zhou K, Huang WT, Zhou P, Yang S, Zhao X, Xie J, Xia L, Ding X (2019) A comprehensive genomic and growth proteomic analysis of antitumor lipopeptide bacillomycin Lb biosynthesis in Bacillus amyloliquefaciens X030. Appl Microbiol Biotechnol 103:7647–7662. https://doi.org/10.1007/s00253-019-10019-6
Luo C, Chen Y, Liu X, Wang X, Wang X, Li X, Zhao Y, Wei L (2019) Engineered biosynthesis of cyclic lipopeptide locillomycins in surrogate host Bacillus velezensis FZB42 and derivative strains enhance antibacterial activity. Appl Microbiol Biotechnol 103:4467–4481. https://doi.org/10.1007/s00253-019-09784-1
Luo Y, Zhang G, Zhu Z, Wang X, Ran W, Shen Q (2013) Optimization of medium composition for lipopeptide production from Bacillus subtilis N7 using response surface methodology. Korean J Microbiol Biotechnol 41(1):52–59. https://doi.org/10.4014/kjmb.1207.07020
Magdalena S, Anggelia Yogiara (2020) Characterization of antibacterial activity produced by Bacillus spp. isolated from honey and other bee-associated products against foodborne pathogens. Bioteknol 17(2):51–59. https://doi.org/10.13057/biotek/c170201
Meena KR, Tandon T, Sharma A, Kanwar SS (2018) Lipopeptide antibiotic production by Bacillus velezensis KLP2016. J Appl Pharm Sci 8(3):91–98. https://doi.org/10.7324/JAPS.2018.8313
Meng QX, Jiang HH, Hanson LE, Hao JJ (2012) Characterizing a novel strain of Bacillus amyloliquefaciens BAC03 for potential biological control application. J Appl Microbiol 113(5):1165–1175. https://doi.org/10.1111/j.1365-2672.2012.05420.x
Miljaković D, Marinković J, Balešević-Tubić S (2020) The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops. Microorganisms 8(1037):1–19. https://doi.org/10.3390/microorganisms8071037
Mizumoto S, Shoda M (2007) Medium optimization of antifungal lipopeptide, iturin A, production by Bacillus subtilis in solid-state fermentation by response surface methodology. Appl Microbiol Biotechnol 76:101–108. https://doi.org/10.1007/s00253-007-0994-9
Mondol MAM, Shin HJ, Islam MT (2013) Diversity of secondary metabolites from marine Bacillus species: chemistry and biological activity. Mar Drugs 11(8):2846–2872. https://doi.org/10.3390/md11082846
Moretta A, Scieuzo C, Petrone AM, Salvia R, Manniello MD, Franco A, Lucchetti D, Vassallo A, Vogel H, Sgambato A, Falabella P (2021) Antimicrobial peptides: a new hope in biomedical and pharmaceutical fields. Front Cell Infect Microbiol 11:668632. https://doi.org/10.3389/fcimb.2021.668632
Motta AS, Lorenzini DM, Brandelli A (2007) Purification and partial characterization of an antimicrobial peptide produced by a novel Bacillus sp. isolated from the Amazon Basin. Curr Microbiol 54:282–286. https://doi.org/10.1007/s00284-006-0414-x
Muhammad N, Akbar A, Shah A, Abbas G, Hussain M, Khan TA (2015) Isolation optimization and characterization of antimicrobial peptide producing bacteria from soil. J Anim Plant Sci 25(4):1107–1113
Nam J, Alam ST, Kang K, Choi J, Seo MH (2020) Anti-staphylococcal activity of a cyclic lipopeptide, C15-bacillomycin D, produced by Bacillus velezensis NST6. J Appl Microbiol 131(1):93–104. https://doi.org/10.1111/jam.14936
Olishevska S, Nickzad A, Déziel E (2019) Bacillus and Paenibacillus secreted polyketides and peptides involved in controlling human and plant pathogens. Appl Microbiol Biotechnol 103:1189–1215. https://doi.org/10.1007/s00253-018-9541-0
Pan H, Tian X, Shao M, Xie Y, Huang H, Hu J, Ju J (2019) Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746. Appl Microb Biotechnol 103(10):4153–4165. https://doi.org/10.1007/s00253-019-09759-2
Panthi S, Choi YH, Jee JP, Cho SS, Choi YS, Pradeep GC, Yoo JC, Suh JW (2017) Antimicrobial peptide from Bacillus strain K1R exhibits ameliorative potential against vancomycin-resistant Enterococcus group of organisms. Int J Pept Res Ther. https://doi.org/10.1007/s10989-016-9572-2
Parachin NS, Mulder KC, Viana AAB, Dias SC, Franco OL (2012) Expression systems for heterologous production of antimicrobial peptides. Pept 38(2):446–456. https://doi.org/10.1016/j.peptides.2012.09.020
Patel PS, Huang S, Fisher S, Pirnik D, Aklonis C, Dean L, Meyers E, Fernandes P, Mayerl F (1995) Bacillaene, a novel inhibitor of procaryotic protein synthesis produced by Bacillus subtilis: production, taxonomy, isolation, physico-chemical characterization and biological activity. J Antibiot 48(9):997–1003. https://doi.org/10.7164/antibiotics.48.997
Perumal V, Yao Z, Kim JA, Kim HJ, Kim JH (2019) Purification and characterization of a bacteriocin, bacBS2, produced by Bacillus velezensis BS2 isolated from Meong-ge Jeotgal. J Microbiol Biotechnol 29(7):1033–1042. https://doi.org/10.4014/jmb.1903.03065
Qian S, Lu H, Meng P, Zhang C, Lv F, Bie X, Lu Z (2015) Effect of inulin on efficient production and regulatory biosynthesis of bacillomycin D in Bacillus subtilis fmbJ. Bioresour Technol 179:260–267. https://doi.org/10.1016/j.biortech.2014.11.086
Qin Y, Wang Y, He Y, Zhang Y, She A, Chai Y, Li P, Shang Q (2019) Characterization of subtilin L-Q11, a novel class I bacteriocin synthesized by Bacillus subtilis L-Q11 isolated from orchard soil. Front Microbiol 10:484. https://doi.org/10.3389/fmicb.2019.00484
Qing-gang G, Li-hong D, Pei-pei W, She-zeng L, Wei-song Z, Xiu-yun L, Xiao-yun Z, Ping M (2018) The PhoR / PhoP two-component system regulates fengycin production in Bacillus subtilis NCD-2 under low-phosphate conditions. J Integr Agric 17(1):149–157. https://doi.org/10.1016/S2095-3119(17)61669-1
Qiu Y, Xiao F, Wei X, Wen Z, Chen S (2014) Improvement of lichenysin production in Bacillus licheniformis by replacement of native promoter of lichenysin biosynthesis operon and medium optimization. Appl Microbiol Biotechnol 98(21):8895–8903. https://doi.org/10.1007/s00253-014-5978-y
Rabbee MF, Baek KH (2020) Antimicrobial activities of lipopeptides and polyketides of Bacillus velezensis for agricultural applications. Mol 25(21):4973. https://doi.org/10.3390/molecules25214973
Rahman FB, Sarkar B, Moni R, Rahman MS (2021) Molecular genetics of surfactin and its effects on different sub-populations of Bacillus subtilis. Biotechnol Rep 32:e00686. https://doi.org/10.1016/j.btre.2021.e00686
Ramachandran R, Chalasani AG, Lal R, Roy U (2014) A broad-spectrum antimicrobial activity of Bacillus subtilis RLID 12.1. Sci World J 2014:1–10. https://doi.org/10.1155/2014/968487
Ravu RR, Jacob MR, Chen X, Wang M, Nasrin S, Kloepper JW, Liles MR, Mead DA, Khan IA, Li XC (2015) Bacillusin A, an antibacterial macrodiolide from Bacillus amyloliquefaciens AP183. J Nat Prod 78(4):924–928. https://doi.org/10.1021/np500911k
Regmi S, Choi YS, Choi YH, Kim YK, Cho SS, Yoo JC, Suh JW (2017) Antimicrobial peptide from Bacillus subtilis CSB138: characterization, killing kinetics, and synergistic potency. Int Microbiol 20(1):45–53. https://doi.org/10.2436/20.1501.01.284
Roessner U, Bowne J (2009) What is metabolomics all about? BioTechniques 46(5):363–365. https://doi.org/10.2144/000113133
Roongsawang N, Washio K, Morikawa M (2011) Diversity of nonribosomal peptide synthetases involved in the biosynthesis of lipopeptide biosurfactants. Int J Mol Sci 12:141–172. https://doi.org/10.3390/ijms12010141
Rungsirivanich P, Parlindungan E, O’Connor PM, Field D, Mahony J, Thongwai N, van Sinderen D (2021) Simultaneous production of multiple antimicrobial compounds by Bacillus velezensis ML122-2 isolated from Assam tea leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]. Front Microbiol 12:1–14. https://doi.org/10.3389/fmicb.2021.789362
Saikia K, Belwal VK, Datta D, Chaudhary N (2019) Aromatic-rich C-terminal region of LCI is a potent antimicrobial peptide in itself. Biochem Biophys Res Commun 519:372–377. https://doi.org/10.1016/j.bbrc.2019.09.013
Sampaio de Oliveira KB, Leite ML, Rodrigues GR, Duque HM, Costa RA, Cunha VA, de Loiola Costa LS, da Cunha NB, Franco OL, Dias SC (2020) Strategies for recombinant production of antimicrobial peptides with pharmacological potential. Expert Rev Clin Pharmacol 13(4):367–390. https://doi.org/10.1080/17512433.2020.1764347
Sass P, Jansen A, Szekat C, Sass V, Sahl HG, Bierbaum G (2008) The lantibiotic mersacidin is a strong inducer of the cell wall stress response of Staphylococcus aureus. BMC Microbiol 9:186. https://doi.org/10.1186/1471-2180-8-186
Scholz R, Molohon KJ, Nachtigall J, Vater J, Markley AL, Süssmuth RD, Mitchell DA, Borriss R (2011) Plantazolicin, a novel microcin B17/streptolysin S-like natural product from Bacillus amyloliquefaciens FZB42. J Bacteriol 193(1):215–224. https://doi.org/10.1128/JB.00784-10
Scholz R, Vater J, Budiharjo A, Wang Z, He Y, Dietel K, Schwecke T, Herfort S, Lasch P, Borriss R (2014) Amylocyclicin, a novel circular bacteriocin produced by Bacillus amyloliquefaciens FZB42. J Bacteriol 196(10):1842–1852. https://doi.org/10.1128/JB.01474-14
Schreiber C, Müller H, Birrenbach O, Klein M, Heerd D, Weidner T, Salzig D, Czermak P (2017) A high-throughput expression screening platform to optimize the production of antimicrobial peptides. Microb Cell Fact 16:29. https://doi.org/10.1186/s12934-017-0637-5
Sharma D, Singh SS, Baindara P, Sharma S, Khatri N, Grover V, Patil PB, Korpole S (2020) Surfactin like broad spectrum antimicrobial lipopeptide co-produced with sublancin from Bacillus subtilis strain A52: dual reservoir of bioactives. Front Microbiol 11:1167. https://doi.org/10.3389/fmicb.2020.01167
Shi J, Zhu X, Lu Y, Zhao H, Lu F, Lu Z (2018) Improving iturin A production of Bacillus amyloliquefaciens by genome shuffling and its inhibition against Saccharomyces cerevisiae in orange juice. Front Microbiol 9:2783. https://doi.org/10.3389/fmicb.2018.02683
Shimizu Y, Ogata H, Goto S (2017) Type III polyketide synthases: functional classification and phylogenomics. ChemBioChem 18(1):50–65. https://doi.org/10.1002/cbic.201600522
Simons A, Alhanout K, Duval RE (2020) Bacteriocins, antimicrobial peptides from bacterial origin: overview of their biology and their impact against multidrug-resistant bacteria. Microorg 8(5):639. https://doi.org/10.3390/microorganisms8050639
Singh V, Haque S, Niwas R, Srivastava A, Pasupuleti M, Tripathi CKM (2017) Strategies for fermentation medium optimization: an in-depth review. Front Microbiol 7:2087. https://doi.org/10.3389/fmicb.2016.02087
So Y, Park SY, Park EH, Park SH, Kim EJ, Pan JG, Choi SK (2017) A highly efficient CRISPR-Cas9-mediated large genomic deletion in Bacillus subtilis. Front Microbiol 8:1167. https://doi.org/10.3389/fmicb.2017.01167
Stein T (2019) Oxygen-limiting growth conditions and deletion of the transition state regulator protein Abrb in Bacillus subtilis 6633 result in an increase in subtilosin production and a decrease in subtilin production. Probiotics Antimicrob Protein 12:725–731. https://doi.org/10.1007/s12602-019-09547-4
Tabbene O, Slimene IB, Djebali K, Mangoni ML, Urdaci MC, Limam F (2009) Optimization of medium composition for the production of antimicrobial activity by Bacillus subtilis B38. Biotechnol Prog 25(5):1267–1274. https://doi.org/10.1002/btpr.202
Tan W, Yin Y, Wen J (2022) Increasing fengycin production by strengthening the fatty acid synthesis pathway and optimizing fermentation conditions. Biochem Eng J 177:108235. https://doi.org/10.1016/j.bej.2021.108235
Tapi A, Chollet-Imbert M, Scherens B, Jacques P (2009) New approach for the detection of non-ribosomal peptide synthetase genes in Bacillus strains by polymerase chain reaction. Appl Microbiol Biotechnol 85(5):1521–1531. https://doi.org/10.1007/s00253-009-2176-4
Théatre A, Cano-Prieto C, Bartolini M, Laurin Y, Deleu M, Niehren J, Fida T, Gerbinet S, Alanjary M, Medema MH, Léonard A, Lins L, Arabolaza A, Gramajo H, Gross H, Jacques P (2021) The surfactin-like lipopeptides from Bacillus spp: natural biodiversity and synthetic biology for a broader application range. Front Bioeng Biotechnol 9:623701. https://doi.org/10.3389/fbioe.2021.623701
Um S, Fraimout A, Sapountzis P, Oh DC, Poulsen M (2013) The fungus-growing termite Macrotermes natalensis harbors bacillaene-producing Bacillus sp. that inhibit potentially antagonistic fungi. Sci Rep 3:3250. https://doi.org/10.1038/srep03250
Vahidinasab M, Lilge L, Reinfurt A, Pfannstiel J, Henkel M, Morabbi Heravi K, Hausmann R (2020) Construction and description of a constitutive plipastatin mono-producing Bacillus subtilis. Microb Cell Fact 19:205. https://doi.org/10.1186/s12934-020-01468-0
van der Hoek SA, Borodina I (2020) Transporter engineering in microbial cell factories: the ins, the outs, and the in-betweens. Curr Opin Biotechnol 66:186–194. https://doi.org/10.1016/j.copbio.2020.08.002
Vargas-Bautista C, Rahlwes K, Straight P (2014) Bacterial competition reveals differential regulation of the pks genes by Bacillus subtilis. J Bacteriol 196(4):717–728. https://doi.org/10.1128/JB.01022-13
Ventola CL (2015) The antibiotic resistance crisis, part 1: causes and threats. P&T: A Peer-Rev J Formul Manag 40(4):277–283. doi:https://doi.org/10.1016/B978-1-4831-9711-1.50022-3
Viel JH, Jaarsma AH, Kuipers OP (2021) Heterologous expression of mersacidin in Escherichia coli elucidates the mode of leader processing. ACS Synth Biol 10:600–608. https://doi.org/10.1021/acssynbio.0c00601
Von Tersch MA, Carlton BC (1983) Bacteriocin from Bacillus megaterium ATCC 19213: comparative studies with megacin A-216. J Bacteriol 155(2):866–871. https://doi.org/10.1128/jb.155.2.866-871.1983
Walsh L, Johnson CN, Hill C, Ross RP (2021) Efficacy of phage- and bacteriocin-based therapies in combatting nosocomial MRSA infections. Front Mol Biosci 8:654083. https://doi.org/10.3389/fmolb.2021.654038
Wang B, Yang B, Peng H, Lu J, Fu P (2022) Genome sequence and comparative analysis of fungal antagonistic strain Bacillus velezensis LJBV19. Folia Microbiol. https://doi.org/10.1007/s12223-022-00996-z
Wang Q, Yu H, Wang M, Yang H, Shen Z (2018) Enhanced biosynthesis and characterization of surfactin isoforms with engineered Bacillus subtilis through promoter replacement and Vitreoscilla hemoglobin co-expression. Process Biochem 70:36–44. https://doi.org/10.1016/j.procbio.2018.04.003
Wei W, Jinjin L, Hai C (2021a) Purification and antimicrobial mechanism of amylocyclicin W5 produced by Bacillus amyloliquefaciens DH8030. Food Sci 42(7):29–34. https://doi.org/10.7506/spkx1002-6630-20200407-082
Wei YH, Wang LC, Chen WC, Chen SY (2010) Production and characterization of fengycin by indigenous Bacillus subtilis F29–3 originating from a potato farm. Int J Mol Sci 11:4526–4538. https://doi.org/10.3390/ijms11114526
Wei Z, Shan C, Zhang L, Ge D, Wang Y, Xia X, Liu X, Zhou J (2021b) A novel subtilin-like lantibiotics subtilin JS-4 produced by Bacillus subtilis JS-4, and its antibacterial mechanism against Listeria monocytogenes. LWT 142:110993. https://doi.org/10.1016/j.lwt.2021.110993
Willenbacher J, Zwick M, Mohr T, Schmid F, Syldatk C, Hausmann R (2014) Evaluation of different Bacillus strains in respect of their ability to produce surfactin in a model fermentation process with integrated foam fractionation. Appl Microbiol Biotechnol 98(23):9623–9632. https://doi.org/10.1007/s00253-014-6010-2
Wohlleben W, Mast Y, Muth G, Röttgen M, Stegmann E, Weber T (2012) Synthetic biology of secondary metabolite biosynthesis in actinomycetes: engineering precursor supply as a way to optimize antibiotic production. FEBS Lett 586:2171–2176. https://doi.org/10.1016/j.febslet.2012.04.025
Wu F, Cai D, Li L, Li Y, Yang H, Li J, Ma X, Chen S (2019a) Modular metabolic engineering of lysine supply for enhanced production of bacitracin in Bacillus licheniformis. Appl Microbiol Biotechnol 103:8799–8812. https://doi.org/10.1007/s00253-019-10110-y
Wu JY, Liao JH, Shieh CJ, Hsieh FC, Liu YC (2018) Kinetic analysis on precursors for iturin A production from Bacillus amyloliquefaciens BPD1. J Biosci Bioeng 126(5):630–635. https://doi.org/10.1016/j.jbiosc.2018.05.002
Wu L, Wu H, Chen L, Yu X, Borriss R, Gao X (2015) Difficidin and bacilysin from Bacillus amyloliquefaciens FZB42 have antibacterial activity against Xanthomonas oryzae rice pathogens. Sci Rep 5:12975. https://doi.org/10.1038/srep12975
Wu Q, Zhi Y, Xu Y (2019b) Systematically engineering the biosynthesis of a green biosurfactant surfactin by Bacillus subtilis 168. Metab Eng 52:87–97. https://doi.org/10.1016/j.ymben.2018.11.004
Xu Y, Cai D, Zhang H, Gao L, Yang Y, Gao J, Li Y, Yang C, Ji Z, Yu J, Chen S (2020) Enhanced production of iturin A in Bacillus amyloliquefaciens by genetic engineering and medium optimization. Process Biochem 90:50–57. https://doi.org/10.1016/j.procbio.2019.11.017
Xu Z, Shao J, Li B, Yan X, Shen Q, Zhang R (2013) Contribution of bacillomycin D in Bacillus amyloliquefaciens SQR9 to antifungal activity and biofilm formation. Appl Environ Microbiol 79(3):808–815. https://doi.org/10.1128/AEM.02645-12
Yaraguppi DA, Bagewadi ZK, Mahanta N, Singh SP, Khan TMY, Deshpande SH, Soratur C, Das S, Saikia D (2022) Gene expression and characterization of iturin A lipopeptide biosurfactant from Bacillus aryabhattai for enhanced oil recovery. Gels 8:403. https://doi.org/10.3390/gels8070403
Yaseen Y, Gancel F, Béchet M, Drider D, Jacques P (2017) Study of the correlation between fengycin promoter expression and its production by Bacillus subtilis under different culture conditions and the impact on surfactin production. Arch Microbiol 199(10):1371–1382. https://doi.org/10.1007/s00203-017-1406-x
Yaseen Y, Diop A, Gancel F, Béchetm M, Jacques P, Drider D (2018) Polynucleotide phosphorylase is involved in the control of lipopeptide fengycin production in Bacillus subtilis. Arch Microbiol 200:783–791. https://doi.org/10.1007/s00203-018-1483-5
Yazici A, Örtücü S, Taşkin M (2021) Screening and characterization of a novel Antibiofilm polypeptide derived from filamentous Fungi. J Proteom 233:104075. https://doi.org/10.1016/j.jprot.2020.104075
Yin H, Guo C, Wang Y, Liu D, Lv Y, Lv F, Lu Z (2013) Fengycin inhibits the growth of the human lung cancer cell line 95D through reactive oxygen species production and mitochondria-dependent apoptosis. Anticancer Drugs 24(6):587–598. https://doi.org/10.1097/CAD.0b013e3283611395
Yu W, Li D, Jia S, Liu Z, Nomura CT, Li J, Chen S, Wang Q (2019) Systematic metabolic pathway modification to boost L-ornithine supply for bacitracin production in Bacillus licheniformis DW2. Appl Microbiol Biotechnol 103:8383–8392. https://doi.org/10.1007/s00253-019-10107-7
Yu X, Xu J, Liu X, Chu X, Wang P, Tian J, Wu N, Fan Y (2015) Identification of a highly efficient stationary phase promoter in Bacillus subtilis. Sci Rep 5:18405. https://doi.org/10.1038/srep18405
Yuan J, Zhao M, Li R, Huang Q, Rensing C, Raza W, Shen Q (2016) Antibacterial compounds-macrolactin alters the soil bacterial community and abundance of the gene encoding PKS. Front Microbiol 7:1904. https://doi.org/10.3389/fmicb.2016.01904
Yue H, Zhong J, Li Z, Zhou J, Yang J, Wei H, Shu D, Luo D, Tan H (2021) Optimization of iturin A production from Bacillus subtilis ZK-H2 in submerge fermentation by response surface methodology. 3 Biotech 11(2):36. https://doi.org/10.1007/s13205-020-02540-7
Zaid DS, Cai S, Hu C, Li Z, Li Y (2022) Comparative genome analysis reveals phylogenetic identity of Bacillus velezensis HNA3 and genomic gnsights into its plant growth promotion and biocontrol effects. Microbiol Spectr 10(1):e0216921. https://doi.org/10.1128/spectrum.02169-21
Zainal Baharin NH, Khairil Mokhtar NF, Mohd Desa MN, Dzaraly ND, Muthanna AR, Al-Obaidi MM, Yuswan MH, Abbasiliasi S, Rahmad N, Wan Nur Ismah WA, Hashim AM, Mustafa S (2022) Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus. Asian Pac J Trop Biomed 12(11):483–494. https://doi.org/10.4103/2221-1691.360564
Zannella C, Chianese A, Palomba L, Marcocci ME, Bellavita R, Merlino F, Grieco P, Folliero V, De Filippis A, Mangoni M, Nencioni L, Franci G, Galdiero M (2022) Broad-spectrum antiviral activity of the amphibian antimicrobial peptide Temporin L and its analogs. Int J Mol Sci 23(4):2060. https://doi.org/10.3390/ijms23042060
Zhang F, Huo K, Song X, Quan Y, Wang S, Zhang Z, Gao W, Yang C (2020a) Engineering of a genome-reduced strain Bacillus amyloliquefaciens for enhancing surfactin production. Microb Cell Fact 19:223. https://doi.org/10.1186/s12934-020-01485-z
Zhang J, Gu S, Zhang T, Wu Y, Ma J, Zhao L, Li X, Zhang J (2022a) Characterization and antibacterial modes of action of bacteriocins from Bacillus coagulans CGMCC 9951 against Listeria monocytogenes. LWT 160:113272. https://doi.org/10.1016/j.lwt.2022.113272
Zhang QY, Yan ZB, Meng YM, Hong XY, Shao G, Ma JJ, Cheng XR, Liu J, Kang J, Fu CY (2021) Antimicrobial peptides: mechanism of action, activity and clinical potential. Mil Med Res 8(48):1–25. https://doi.org/10.1186/s40779-021-00343-2
Zhang Q, Kobras CM, Gebhard S, Mascher T, Wolf D (2022b) Regulation of heterologous subtilin production in Bacillus subtilis W168. Microb Cell Fact 21:57. https://doi.org/10.1186/s12934-022-01782-9
Zhang W, Wei L, Xu R, Lin G, Xin H, Lv Z, Qian H, Shi H (2020b) Evaluation of the antibacterial material production in the fermentation of Bacillus amyloliquefaciens-9 from Whitespotted bamboo shark (Chiloscyllium plagiosum). Mar Drugs 18:119. https://doi.org/10.3390/md18020119
Zhang X, Guo X, Wu C, Li C, Zhang D, Zhu B (2020c) Isolation, heterologous expression, and purification of a novel antifungal protein from Bacillus subtilis strain Z-14. Microb Cell Fact 19:214. https://doi.org/10.1186/s12934-020-01475-1
Zhang Y, Chen M, Bruner SD, Ding Y (2018) Heterologous production of microbial ribosomally synthesized and post-translationally modified peptides. Front Microbiol 9:1801. https://doi.org/10.3389/fmicb.2018.01801
Zhao J, Li Y, Zhang C, Yao Z, Zhang L, Bie X, Lu F, Lu Z (2012) Genome shuffling of Bacillus amyloliquefaciens for improving antimicrobial lipopeptide production and an analysis of relative gene expression using FQ RT-PCR. J Ind Microbiol Biotechnol 39:889–896. https://doi.org/10.1007/s10295-012-1098-9
Zhao J, Zhang C, Lu J, Lu Z (2016) Enhancement of fengycin production in Bacillus amyloliquefaciens by genome shuffling and relative gene expression analysis using RT-PCR. Can J Microbiol 62(5):431–436. https://doi.org/10.1139/cjm-2015-0734
Zhao X, Han Y, Tan X, Wang J, Zhou Z (2014) Optimization of antifungal lipopeptide production from Bacillus sp. BH072 by response surface methodology. J Microbiol 52(4):324–332. https://doi.org/10.1007/s12275-014-3354-3
Zhao X, Kuipers OP (2016) Identification and classification of known and putative antimicrobial compounds produced by a wide variety of Bacillales species. BMC Genom 17:882. https://doi.org/10.1186/s12864-016-3224-y
Zhou S, Liu G, Zheng R, Sun C, Wu S (2020) Structural and functional insights into iturin W, a novel lipopeptide produced by the deep-sea bacterium Bacillus sp. strain wsm-1. Appl Environ Microbiol 86:e01597-e1620. https://doi.org/10.1128/AEM.01597-20
Zhu J, Cai D, Xu H, Liu Z, Zhang B, Wu F, Li J, Chen S (2018) Enhancement of precursor amino acid supplies for improving bacitracin production by activation of branched chain amino acid transporter BrnQ and deletion of its regulator gene lrp in Bacillus licheniformis. Synth Syst Biotechnol 3:236–243. https://doi.org/10.1016/j.synbio.2018.10.009
Zhu J, Li L, Wu F, Wu Y, Wang Z, Chen X, Li J, Cai D, Chen S (2021) Metabolic engineering of aspartic acid supply modules for enhanced production of bacitraicn in Bacillus licheniformis. ACS Synth Biol 10(9):2243–2251. https://doi.org/10.1021/acssynbio.1c00154
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This research was supported by the Ministry of Higher Education Malaysia under the Fundamental Research Grant Scheme (FRGS/1/2021/STG01/UPM/02/1) and the National Research Foundation of Korea (NRF) grant (No. 2021R1G1A1010154) funded by the Korea government (MSIT). PSL and PE were sponsored by a Graduate Research Fellowship and Special Graduate Research Allowance Scheme from Universiti Putra Malaysia, respectively.
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Puan, S.L., Erriah, P., Baharudin, M.M.Aa. et al. Antimicrobial peptides from Bacillus spp. and strategies to enhance their yield. Appl Microbiol Biotechnol 107, 5569–5593 (2023). https://doi.org/10.1007/s00253-023-12651-9
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DOI: https://doi.org/10.1007/s00253-023-12651-9