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Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants

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Abstract

A reliable protocol has been established for in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood), a valuable medicinal plant from India. A highly proliferating organogenic callus was obtained on Murashige and Skoog (MS) medium supplemented with 2.5 µM IAA when nodal explants were cultured on MS medium supplemented with various growth regulators. Further, highest regeneration frequency (83.3 %) of adventitious shoots was observed, when the callus was sub-cultured on MS medium supplemented with 6-benzylaminopurine (BAP; 2.5 µM) along with 7.5 µM 2-isopentenyl adenine (2-iP). An optimal of 10.16 ± 2.24 shoots were regenerated on medium supplemented with 2.5 µM BAP + 7.5 µM 2-iP. Quarter strength MS medium supplemented with 10 µM IBA was effective for rooting of the shoots. Ex-vitro plants were normal and were established successfully. Cytological and molecular marker studies showed that regenerated plants showed genetic stability in micro-propagated plants.

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Acknowledgments

This work was supported partially by BRNS project (BRNS Project No. 2013/35/6/BRNS/20) and DST-PURSE-Phase-2 program.

Authors' contribution

HNM designed the experiment, SS, KSJ, JJ, and SHM conducted the experiment. All the authors together written and approved the manuscript.

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Authors and Affiliations

  1. Department of Botany, Karnatak University, Dharwad, 580003, India

    Smita Shinde, Joseph Kadanthottu Sebastian, Manohar Shirugumbi Hanamanthagouda & Hosakatte Niranjana Murthy

  2. Department of Biotechnology, Jain University, Bengaluru, 560069, India

    Jyothi Ramesh Jain & Manohar Shirugumbi Hanamanthagouda

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  1. Smita Shinde
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  2. Joseph Kadanthottu Sebastian
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  3. Jyothi Ramesh Jain
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  4. Manohar Shirugumbi Hanamanthagouda
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  5. Hosakatte Niranjana Murthy
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Correspondence to Hosakatte Niranjana Murthy.

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Shinde, S., Sebastian, J.K., Jain, J.R. et al. Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants. Physiol Mol Biol Plants 22, 595–603 (2016). https://doi.org/10.1007/s12298-016-0379-6

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  • DOI: https://doi.org/10.1007/s12298-016-0379-6

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