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Conservation of medicinal plants of Western Ghats, India and its sustainable utilization through in vitro technology

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Abstract

Climate change, alien species, and use of land for intensive farming and development are causing severe threat to the plant genetic diversity worldwide. Hence, conservation of biodiversity is considered fundamental and also provides the livelihoods to millions of people worldwide. Medicinal plants play a key role in the treatment of a number of diseases, and they are only the source of medicine for majority of people in the developing world. The tropical regions of the world supply the bulk of current global demand for “natural medicine,” albeit with increasing threat to populations in the world and its genetic diversity. India is a major center of origin and diversity of crop and medicinal plants. India poses out 20,000 species of higher plants, one third of it being endemic and 500 species are categorized to have medicinal value. The Western Ghats is one of the major repositories of medicinal plants. It harbors around 4,000 species of higher plants of which 450 species are threatened. Currently, the number of species added to the red list category in this region is increasing, and the valuable genetic resources are being lost at a rapid rate. Demand for medicinal plants is increasing, and this leads to unscrupulous collection from the wild and adulteration of supplies. Providing high-quality planting material for sustainable use and thereby saving the genetic diversity of plants in the wild is important. During the last 25 years of intensive research, Tropical Botanic Garden and Research Institute has developed in vitro protocol for rapid regeneration and establishment of about 40 medicinally important rare and threatened plants of Western Ghats. In situ conservation alone would not be effective in safeguarding these important species. Thus, utilizing the biotechnoligical approach to complement ex situ conservation program is becoming vital. Propagating biotechnology tools in plant conservation program is a prerequisite to succeed in sustainable use and to complement the existing ex situ measures. In addition to propagation, storage of these valuable genetic resources is equally important. In vitro slow growth of 35 species and cryopreservation using embryo/meristem/seed in 20 different species of rare medicinal plants of this region is accomplished. Plants developed in vitro of ten medicinal plants, which have restricted distribution, were reintroduced in the natural habitat as well.

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References

  • Ajithkumar D.; Seeni S. Rapid clonal multiplication through in vitro axillary shoot proliferation of Aegle marmelos (L.) Corr., a medicinal tree. Plant Cell Rep 17: 422–426; 1998.

    Article  CAS  Google Scholar 

  • Anand A. Studies on genetic stability of micropropagated plants and reintroduction in an endemic and endangered taxon: Syzygium travancoricum Gamble. J Plant Biotechnol 5: 201–207; 2003.

    Google Scholar 

  • Anand M. P. H.; Harikrishnan K. N.; Martin K. P.; Hariharan M. In vitro propagation of Kaempferia rotunda Linn. "Indian crocus": a medicinal plant. Phytomorphology 47: 281–286; 1997.

    Google Scholar 

  • Animesh B.; Bari M. A.; Mohashweta R.; Bhadra S. K. In vitro regeneration of Aristolochia tagala CHAMP. - A rare medicinal plant of chittagong hill tracts. J Biosci 15: 63–67; 2007.

    Google Scholar 

  • Anish N. P.; Dan M.; Bejoy M. Conservation using in vitro progenies of the threatened Ginger Boesenbergia pulcherrima (Wall.) Kuntze. Int J Bot 4: 93–98; 2008.

    Article  CAS  Google Scholar 

  • Anitha S.; Kumari B. D. R. Stimulation of reserpine biosynthesis in the callus of Rauvolfia tetraphylla L. by precursor feeding. Afr J Biotechnol 5: 659–661; 2006.

    CAS  Google Scholar 

  • Anu A.; Babu K.; Nirmal John C. Z.; Peter K. V. In vitro clonal Multiplication of Acorus calamus L. J Plant Biochem Biotechnol 10: 53–55; 2001.

    CAS  Google Scholar 

  • Asha K. I.; Nair G. M. Direct Organogenesis in Dioscorea Species. Indian J Plant Genet Resour 18: 250–257; 2005.

    Google Scholar 

  • Asha K. I.; Nair G. M. Shoot Multiplication in Dioscorea Species. Indian J Plant Genet Resour 20: 130–136; 2007.

    Google Scholar 

  • Avik R.; Sabita B. Cryopreservation of in vitro grown nodal segments of Rauvolfia serpentina by pvs2 vitrification. Cryoletters 29: 321–328; 2008.

    Google Scholar 

  • Baksha R.; Miskat A. A. J.; Rahima K.; John L. M. In vitro rapid clonal propagation of Rauvolfia serpentina (Linn.) Benth. Bangladesh. J Sci Ind Res 42: 37–44; 2007.

    Google Scholar 

  • Becker H.; Sauerwein M. Manipulation of the biosynthetic capacity of plant cell structures In: Chadwood RV. In: Rhodon M. J. C. (ed) Secondary products from plant tissue culture. Clerendan Dvers, Oxford, pp 43–58; 1990.

    Google Scholar 

  • Bejoy M.; Dan M.; Anish M. P. Factors affecting the in vitro multiplication of the endemic ginger, Curcuma haritha Mangaly and Sabu. Asian J Plant Sci 5: 847–853; 2006.

    Article  Google Scholar 

  • Benjamin B. D.; Roja G.; Heble M. R. Agrobacterium mediated transformation of Rauvolfia serpentina: regeneration and alkaloid synthesis. Plant Cell Tissue Organ Cult 35: 253–257; 1993.

    Article  CAS  Google Scholar 

  • Benoy J.; Satheeshkumar K. In vitro mass multiplication of Ophiorrhiza mungo Linn. Indian J Exp Biol 42: 639–642; 2004.

    Google Scholar 

  • Benoy J.; Satheeshkumar K.; Seeni S. A protocol for high frequency regeneration through nodal explant cultures and ex vitro rooting of Plumbago rosea L Pakistan. J Biol Sci 10: 349–355; 2007.

    Google Scholar 

  • Biotech Consortium India Limited. Summary report on market survey on tissue cultured plants. Department of Biotechnology, Government of India and Small, Farmers’ Agri-Business consortium.http://dbtmicropropagation.nic.in/surveytcp.pdf: pp.1–14; 2005.

  • Chaudhury R.; Chandel K. P. S. Germination studies and cryopreservation of seeds of black pepper (Piper nigrum L.)- a recalcitrant species. Cryoletters 15: 145–150; 1994.

    Google Scholar 

  • Chaturvedi H. C.; Jain M.; Kidwai N. R. Cloning of medicinal plants through tisuue culture-A review. Indian J Exp Biol 45: 937–948; 2007.

    PubMed  CAS  Google Scholar 

  • Chithra M.; Martin K. P.; Sunandakumari C.; Madhusoodanan P. V. Silver nitrate induced rooting and flowering in vitro of rare rhoeophytic woody medicinal plant, Rotula aquatica Lour. Indian J Biotechnol 3: 418–421; 2004.

    CAS  Google Scholar 

  • Chithra M.; Martin K. P.; Sunandakumari C.; Madhusoodanan P. V. Protocol for rapid propagation, and to overcome delayed rhizome formation in field established in vitro derived plantlets of Kaempferia galanga L. Sci Hortic 15: 113–120; 2005.

    Article  CAS  Google Scholar 

  • D’Souza L.; Rajendra K.; Vinitha C.; Alice C. A.; Smitha H.; Icy D. Micropropagation and conservation of medicinal plants. In: Khan I. A.; Khanum A. (eds) Role of Biotechnology in medicinal and aromatic plants. Ukaaz, Hyderabad, pp 57–82; 1998.

    Google Scholar 

  • Decruse S. W.; Gangaprasad A.; Seeni S.; Sarojini Menon V. Micropropagation and ecorestoration of Vanda spthulata, an exquisite orchid. Plant Cell Tissue Organ Cult 72: 199–2002; 2003.

    Article  Google Scholar 

  • Decruse S. W.; Seeni S. Pushpangadan, P. Cryopreservation of alginate coated shoot tips of in vitro grown Holostemma annulare (Roxb.) K. Schum, an endangered medicinal plant: Influence of preculture and DMSO treatment on survival and regeneration. Cryoletters 20: 243–250; 1999.

    Google Scholar 

  • Decruse S. W.; Seeni S.; Pushpangadan P. Effect of cryopreservation on seed germination of selected rare medicinal plants of peninsular India. Seed Sci Tech 27: 501–505; 1999.

    Google Scholar 

  • Decruse S. W.; Seeni S. Ammonium nitrate in the culture medium influences regeneration potential of cryopreserved shoot tips of Holostemma annulare. Cryoletters 23: 55–60; 2002.

    PubMed  Google Scholar 

  • Decruse S. W.; Seeni S. Seed cryopreservation is a suitable storage procedure for a range of Piper species. Seed Sci Tech 31: 213–217; 2003.

    Google Scholar 

  • Decruse S. W.; Seeni S.; Nair G. M. Preparative procedures and culture media effect on the success of cryostorage of Holostemma annulare shoot tips. Plant Cell Tissue Organ Cult 76: 179–182; 2004.

    Article  Google Scholar 

  • Delse P. S.; Sailas B.; Molly H. Micropropagation of Rotula aquatica Lour.-an important woody mediicnal plant. Phytomorphology 52: 137–144; 2002.

    Google Scholar 

  • Dennis T.; Surabhi S. Multiple shoot induction and callus regeneration in Sarcostemma brevistigma Wight and Arnott, a rare medicinal plant. Plant Biotechnol Rep 3: 67–74; 2009.

    Article  Google Scholar 

  • Engelmann F. Importance of cryopreservation for the conservation of plant genetic resources. In: Engelmann F.; Takagi H. (eds) Cryopreservation of tropical plant germplasm. Current research progress and application. IPGRI, Rome, pp 8–20; 2000.

    Google Scholar 

  • Faisa M.; Ahmad N.; Anis M. An efficient micropropagation system for Tylophora indica: an endangered, medicinally important plant. Plant Biotechnol Rep 1: 155–161; 2007.

    Article  Google Scholar 

  • Falk D. A.; Knapp E.; Guerrant E. O. An introduction to restoration genetics. Society for Ecological Restoration, US Environment Protection Agency, Washington DC; 2001.

    Google Scholar 

  • Falkenhagen H.; Kuzovkina I. N.; Alterman I. E.; Nikolaeva L. A.; Stockigt J. Alkaloid formation in hairy roots and cell suspensions of Rauwolfia serpentina Benth. Nat Prod Res 3: 107–112; 1993.

    Article  CAS  Google Scholar 

  • Fay M. F. Conservation of rare and endangered plants using in vitro methods. In Vitro Cell Dev Biol Plant 28: 1–4; 1992.

    Google Scholar 

  • Fay M. F. In what situations is in vitro culture appropriate to plant conservations? Biodivers Conserv 3: 176–183; 1994.

    Article  Google Scholar 

  • Fulzele D. P.; Satdive R. K.; Pol B. B. Growth and production of camptothecin by cell suspension cultures of Nothapodytes foetida. Planta Med 67: 150–152; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Fulzele D. P.; Satdive R. K.; Pol B. B. Untransformed root cultures of Nothapodytes foetida and production of camptothecin. Plant Cell Tissue Organ Cult 69: 285–288; 2002.

    Article  CAS  Google Scholar 

  • Gangaprasad A.; Decruse S. W.; Seeni S.; Nair G. M. Micropropagation and restoration of Decalepis arayalpathra (Joseph and Chandra) Venter- an endemic and endangered ethanomedicinal plant of Western Ghats. Indian J Biotechnol 4: 265–270; 2005.

    CAS  Google Scholar 

  • Gangaprasad A.; Decruse S. W.; Seeni S.; Sarojini Menon V. Micropropagation and restoration of the endangered malabar daffordil orchid Ipsea malabarica (Reich.b.f.) Hook.f. Lindleyana 14: 38–46; 1998.

    Google Scholar 

  • Gangaprasad A.; Lakshmi G. N.; Radhakrishnan K.; Seeni S.; Nair G. M.; Pushpangadan P. Micropropagation of Utleria salicifolia,- an endemic endangered ethanomedicinal plant of the Western Ghats. J Med Aromatic Plant Sci 25: 19–24; 2003.

    Google Scholar 

  • George S.; Geetha S. P.; Anu A.; Indira B. In vitro conservation studies in Hemidesmus indicus, Decalepis hamiltonii and Utleria salicifoliaIn:Proceedings of the 22nd Kerala Science Congress, Kerala State Council for Science Technology and Environment, Thiruvnanthapuram, Kerala, India: 258–259; 2010.

  • Gerald M.; Geetha S. P.; Sudhakar S. R.; Raghu A. V.; Indira B.; Ravindran A. N. An efficient micropropagation system for Celastrus paniculatus Willd.: a vulnerable medicinal plant. J For Res 11: 461–465; 2006.

    Article  Google Scholar 

  • Gerald M.; Geetha S. P.; Raghu A. V.; Indira B.; Ravindran P. N. Micropropagation of Holarrhena pubescens. J Trop Med Plants 6: 111–118; 2005.

    Google Scholar 

  • Ghosh B.; Mukherjee S.; Jha T. B.; Jha S. Enhanced colchicine production in root cultures of Gloriosa superba by direct and indirect precursors of the biosynthetic pathway. Biotechnol Lett 24: 231–234; 2002.

    Article  CAS  Google Scholar 

  • Ghosh S.; Ghosh B.; Jha S. Aluminium chloride enhances colchicine production in root cultures of Gloriosa superba. Biotechnol Lett 28: 497–503; 2006.

    Article  PubMed  CAS  Google Scholar 

  • Gilani A. H. Role of medicinal plants in modern medicine. Malays J Sci 24: 1–5; 2005.

    Google Scholar 

  • Giridhar P.; Gururaj H. B.; Ravishankar G. A. In vitro shoot multiplication through shoot tip cultures of Decalepis hamiltonii Wight and Arn., a threatened plant endemic to Southern India. In Vitro Cell Dev Biol Plant 41: 77–80; 2005.

    Google Scholar 

  • Gokhale M.; Bansal Y. K. Direct in vitro regeneration of a medicinal tree Oroxylum indicum (L.) Vent. through tissue culture. Afr J Biotechnol 8: 3777–3781; 2009.

    CAS  Google Scholar 

  • Gopi C.; Vatsala T. M. In vitro studies on effects of plant growth regulators on callus and suspension culture biomass yield from Gymnema sylvestre R.Br. Afr J Biotechnol 5: 1215–1219; 2006.

    CAS  Google Scholar 

  • Grell E.; Schmude H. N. F.; Lamb A.; Bacon A. Re-introducing Paphiopedilum rothchildianum to Sabah, North Borneo. Am Orchid Soc Bull 57: 1238–1245; 1988.

    Google Scholar 

  • Hayashi T.; Kasahara K.; Sankawa U. Efficient production of biologically active diterpenoid by leaf organ culture of Scoparia dulcis. Cell Cult Biotechnol 46: 517–520; 1998.

    Google Scholar 

  • Hettiarachchi A.; Fernando K. K. S.; Jayasurya A. H. M. In vitro propagation of Wadakaha (Acorus calamus L). J Natn Sci Coun SriLanka 25: 151–157; 1997.

    Google Scholar 

  • Hilton M. G.; Rhodes M. J. C. Factors affecting the growth and hyocyamine production during batch culture of transformed roots of Datura stramonium. Planta Med 59: 340–344; 1993.

    Article  PubMed  CAS  Google Scholar 

  • Indira Iyer R.; Jayaraman G.; Ramesh A. Direct somatic embryogenesis in Myristica malabarica Lam., an endemic, threatened medicinal species of Southern India and detection of phytochemicals of potential medicinal value. Indian J Sci Technol 2: 11–17; 2009.

    CAS  Google Scholar 

  • Islam R.; Karim M. R. Plant regeneration from excised cotyledon of Aegle marmelos Corr. Pak J Bot 26: 393–396; 1994.

    Google Scholar 

  • Jain M.; Rathore A. K.; Khanna P. Influence of kinetin and auxins on the growth and production of diosgenin by Costus speciosus (Koen.) Sm. callus derived from rhizome. Agric Biol Chem 48: 529; 1984.

    CAS  Google Scholar 

  • Jayakumaran Nair A.; Sudhakaran P. R.; Madhusudana Rao J.; Ramakrishna S. V. Berberine synthesis by callus and cell suspension cultures of Coscinium fenestratum. Plant Cell Tissue Organ Cult 29: 7010; 1992.

    Article  Google Scholar 

  • Johnson M.; Alias A. Somoclonal variation studies on Phyllanthus amarus Schum and Thonn Iran. J Biotechnol 5: 240–245; 2007.

    Google Scholar 

  • Joy P. P.; Thomas J.; Mathew S.; Skaria B. P. (2001) Scope of medicinal, aromatic and dye yielding crops in Kerala. In: National seminar cum workshop on “Scope of medicinal, aromatic and dye yielding crops in dry land agriculture.” Central Research Institute for Dryland Agriculture, Hyderabad, India; 11–13 Dec

  • Kameswara Rao N. Plant genetic resources: advancing conservation and use through biotechnology. Afr J Biotechnol 3: 136–145; 2004.

    Google Scholar 

  • Karmarkar S. H.; Keshavachandran R.; Nazeem P. A.; Girija D. Hairy root induction in adapathiyan (Holostemma ada-kodien K.Schum.). J Trop Agric 39: 102–107; 2001.

    Google Scholar 

  • Karthikeyan K.; Chandran C.; Kulothungan S. Rapid clonal multiplication through in vitro axillary shoot proliferation of Centella asiatica. Indian J Biotehnol 8: 232–235; 2009.

    CAS  Google Scholar 

  • Karuppusamy S. A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. J Med Plant Res 3: 1222–1239; 2009.

    CAS  Google Scholar 

  • Khan T.; Krupadanam D.; Anwar S. Y. The role of phytohormone on the production of berberine in the calli cultures of an endangered medicinal plant, turmeric (Coscinium fenestratum l.). Afr J Biotechnol 7: 3244–3246; 2008.

    CAS  Google Scholar 

  • Kim O. T.; Bang K. H.; Shin Y. S.; Lee M. J.; Jang S. J.; Hyun D. Y.; Kim Y. C.; Senong N. S.; Cha S. W.; Hwang B. Enhanced production of asiaticoside from hairy root cultures of Centella asitica (L.) Urban elicited by methyl jasmonate. Plant Cell Rep 26: 1914–1949; 2007.

    Google Scholar 

  • Kirillova K. V.; Smirnova M. G.; Komov V. P. Sequential isolation of superoxide dismutase and ajmaline from tissue cultures of Rauwolfia serpentina Benth. Appl Biochem Microbiol 37: 160–163; 2001.

    Article  CAS  Google Scholar 

  • Krishnan P. N.; Sudha C. G.; Seeni S. Rapid propagation through shoot tip culture of Trichopus zeylanicus Gaertn., a rare ethnomedicinal plant. Plant Cell Rep 14: 708–711; 1995.

    Article  CAS  Google Scholar 

  • Krombholz R.; Mersinger R.; Kreis W.; Reinhard E. Production of forskolin by axenic Coleus forskolhii roots cultivated in shake flasks and 20 L glass jar bioreactors. Planta Med 58: 328–333; 1992.

    Article  PubMed  CAS  Google Scholar 

  • Laksmi G. N.; Seeni S. Micropropagation and restoration of Blepharestemma membranifolia (Miq.) Ding Hon., an endemic and threatened medicinal tree of the Western Ghats. In: Proceedings of the 13th Kerala Science Congress, Kerala State Council for Science Technolgy and Environment, Thiruvananthapuram, Kerala, India: 124–128; 2001a.

  • Laksmi G. N.; Seeni S. Rapid in vitro multiplication and field establishment of Celastrus paniculatus sub sp. paniculatus Willd. (Celastraceae), a medicinal woody climber. Indian J Exp Biol 39: 697-–704; 2001b.

    Google Scholar 

  • Lakshmi G. N.; Seeni S. Rapid clonal multiplication of Morinda umbellata Linn (Rubiaceaqe), a medicinal liana through culture of nodes and shoot tips from mature plant. Phytomorphology 52: 77–81; 2002.

    Google Scholar 

  • Lakshmi G. N.; Seeni S. In vitro multiplication of Calophyllum apetalum (Clusiaceae), an endemic medicinal tree of the Western Ghats. Plant Cell Tissue Organ Cult 75: 169–174; 2003.

    Article  Google Scholar 

  • Lincy K. A.; Remashree A. B.; Sasikumar B. Indirect and direct somatic embryogenesis from aerial stem explants of ginger (Zingiber officinale Rosc.). Acta Bot Croat 68: 93–103; 2009.

    CAS  Google Scholar 

  • Loc N.; Doan D.; Tae K.; Moon Y. Micropropagation of zedoary (Curcuma zedoaria Roscoe)– a valuable medicinal plant. Plant Cell Tissue Organ Cult 81: 119–122; 2005.

    Article  CAS  Google Scholar 

  • McGlaughlin M.; Karoly K.; Kaye T. Genetic variation and its relationship to population size in reintroduced populations of pink sand verbena, Abronia umbellata subsp. breviflora (Nyctaginaceae). Conserv Genet 3: 411–420; 2002.

    Article  CAS  Google Scholar 

  • Malabadi R. B.; Mulgund G. S.; Nataraja K. Effect of triacontanol on the micropropagation of Costus speciosus (Koen.) SM. using rhizome thin sections. In Vitro Cell Dev Biol Plant 41: 129–132; 2005.

    Article  CAS  Google Scholar 

  • Malik S. K.; Chaudhury R.; Kalia R. K. Rapid in vitro multiplication and conservation of Garcinia indica: A tropical medicinal tree species. Sci Hortic 106: 539–553; 2005.

    Article  Google Scholar 

  • Mandal B. B.; Chandel K. P. S.; Dwivedi S. Cryopreservation of yams (Dioscorea spp.) shoot apices by encapsulation-dehydration. Cryoletters 17: 165–174; 1996.

    Google Scholar 

  • Mandal B. B.; Sharma D. S.; Srivastava P. S. Embryogenic Cultures of Dioscorea bulbifera L. by encapsulation-dehydration. Cryoletters 30: 440–448; 2009.

    PubMed  CAS  Google Scholar 

  • Martin K. P. Rapid propagation of Holostemma ada-kodien Schult., a rare medicinal plant, through axillary bud multiplication and indirect organogenesis. Plant Cell Rep 21: 112–117; 2002.

    Article  CAS  Google Scholar 

  • Martin K. P. Clonal propagation, encapsulation and reintroduction of Ipsea malabarica (Reichb. F.) J. D. Hook., an endangered orchid. In Vitro Cell Dev Biol Plant 39: 322–326; 2003.

    Article  CAS  Google Scholar 

  • Massot B.; Milesi S.; Gontier E.; Bourgaud F.; Guckert A. Optimized culture conditions for the production of furanocoumarins by micropropagated shoots of Ruta graveolens. Plant Cell Tissue Organ Cult 62: 11–19; 2000.

    Article  CAS  Google Scholar 

  • Maunder M. Plant reintroduction: an overview. Biodivers Conserv 1: 51–62; 1992.

    Article  Google Scholar 

  • Muthukumar B.; Arockiasamy D. I.; Natarajan E. Direct organogenesis in Datura metel L., from in vitro nodal explants. Indian J Biotechnol 3: 449–451; 2004.

    CAS  Google Scholar 

  • Natesh S. Conservation of medicinal and aromatic plants in India- an overview.In: Kamaruddin MS. In: Natesh S.; Osman A.; Azizol A. K. (eds) Medicinal and aromatic plants: strategies and technologies for conservation. Forest Research Institute, Kuala Lumpur, pp 1–11; 1999.

    Google Scholar 

  • Nath T. K.; Chandra S. N.; Tiwari V.; Deo Singh B. Micropropagation of Centella asiatica (L.), a valuable medicinal herb. Plant Cell Tissue Organ Cult 63: 179–185; 2000.

    Article  Google Scholar 

  • Neelam S.; Chandel K. P. S.; Srivastava V. K. In vitro propagation of Coleus forskohlii Briq., a threatened medicinal plant. Plant Cell Rep 10: 67–70; 1991.

    Google Scholar 

  • Neelam S.; Vimala D. S.; Ruchira P. In vitro propagation of a threatened, anticarcinogenic herb Curculigo orchioides Gaertn. J Plant Biochem Biotechnol 16: 63–65; 2007.

    Google Scholar 

  • Neelam S.; Chandel K. P. S. Low-temperature storage of Rauvolfia serpentina Benth. Ex Kunrz.- an endangered, endemic medicinal plant. Plant Cell Rep 11: 200–203; 1992.

    Google Scholar 

  • Nikam T. D.; Savant S. R. Callus culture and micropropagation of Ceropegia sahyadrica Ans. and Kulk.: an edible starchy tuberous rare asclepiad. Indian J Plant Physiol 12: 178–180; 2007.

    Google Scholar 

  • Nikam T. D.; Savant S. R. Multiple shoot regeneration and alkaloid cerpegin accumulation in callus culture of Ceropegia juncea Roxb. Physiol Mol Biol Plants 15: 71–77; 2009a.

    Article  CAS  Google Scholar 

  • Nikam T. D.; Ebrahami M. A.; Patil V. A. Embryogenic callus culture of Tribulus terrestris L. a potential source of harmaline, harmine and diosgenin. Plant Biotechnol Rep 3: 243–250; 2009b.

    Article  Google Scholar 

  • Nisha A.; Narasimhan S.; Manjula S.; Nair G. M. Direct Organogenesis in Geophila reniformis D.Don, an important medicinal herb. J Plant Biotechnol 6: 189–192; 2004.

    Google Scholar 

  • Nishritha B.; Sanjay S. In vitro propagation of a high value medicinal plant: Asparagus racemosus Willd. In Vitro Cell Dev Biol-Plant 44: 525–432; 2008.

    Article  Google Scholar 

  • Noman A. S. M.; Islam M. S.; Siddique N. A.; Hossain K. High frequency induction of multiple shoots from nodal explants of Vitex negundo using silver nitrate. Int J Agric Biol 10: 633–637; 2008.

    CAS  Google Scholar 

  • Pal A.; Roy A. Embryo culture of Costus speciosus (Koen.) Sm. to regenerate variable diosgenin yielding clones. Plant Cell Rep 10: 565–568; 1991.

    Article  Google Scholar 

  • Papiya M.; Mandal B. B.; Bhat K. V.; Biswas A. K. Cryopreservation of Asian Dioscorea bulbifera L. and D. alata L. by vitrification: importance of plant growth regulators. Cryoletters 30: 110–111; 2009.

    Google Scholar 

  • Prakash S.; Elangomathavan R.; Seshadri S.; Kathiravan K.; Ignacimuthu S. Efficient regeneration of Curcuma amada Roxb. plantlets from rhizome and leaf sheath explants. Plant Cell Tissue Organ Cult 78: 159–165; 2004.

    Article  CAS  Google Scholar 

  • Prathanturarug S.; Soonthornchareonnon N.; Chuakul W.; Phaidee Y.; Saralamp P. Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron supplemented liquid medium. Plant Cell Tissue Organ Cult 80: 347–351; 2005.

    Article  CAS  Google Scholar 

  • Preetha T. S.; Hemanthakumar A. S.; Decruse S. W.; Krishnan P. N.; Seeni S. Effect of synthetic auxins on somatic embryogenesis from leaf derived callus of Kaempferia galanga L- an endangered medicinal plant. Phytomorphology 58: 117–124; 2008.

    Google Scholar 

  • Pushpangadan P.; Seeni S.; Jacob T. Conservation of plant genetic resources in India- establishment of gene bank for medicinal and aroatic plants at Tropical Botanic Garden and Reserach Institute. G-15 GEMAB Newsletter Nos 5 and 6: 10-12; 1994.

  • Radha R. K.; Seeni S. (2010c) In vitro propagation and regeneration of Mahonia leschenaultii Nutt. a tree species of Western Ghats. In vitroCell Dev Biol-Plant (in press)

  • Radha R. K.; Shereena S. R.; Divya K.; Krishnan P. N.; Seeni S. (2010a) In vitro propagation from seedling explants and anthaquinone production from in vitro shoots of Rubia cordifolia Linn., a medicinal plant. 97th Indian Science Congress, University of Kerala, Kariavattom, India.3–7 Jan

  • Radha R. K.; William Decruse S.; Amy M. V.; Krishnan P. N. (2010b) Zygotic embryo cryopreservation of Celastrus paniculatus, Golden Jubilee National Symposium on Plant Diversity Utilization and Management, Department of Botany, University of Kerala, Kariavattom, India. 27–29 May

  • Radha R. K.; William Decruse S.; Krishnan P. N. Cryopreservation of excised embryonic axes of Nothapodytes nimmoniana (Graham) Mebberly, a vulnerable tree species of the Western Ghats Indian J Biotechnol. Indian J Biotechnol 9:435-437; 2010d.

    Google Scholar 

  • Radha R. K.; William Decruse S.; Seeni S.; Ganeshan S. (2006) Cryopreservation of embryonic axes of recalcitrant seed species Myristica malabarica Lam., a rare medicinal plant of the Western Ghats. National Seminar on Plant Resources of Western Ghats. Indian Institute of Science, Bangalore, Karnataka, India 7–8 December

  • Raghu A. V.; Geetha S. P.; Gerald M.; Indira B.; Ravindran P. N. In vitro clonal propagation through mature nodes of Tinspora cordifolia (Willd.) Hook.f. and Thomas.: an important ayurvedic medicinal plant. In vitro Cell Dev Biol Plant 42: 504–508; 2006a.

    Google Scholar 

  • Raghu A. V.; Geetha S. P.; Gerald M.; Indira B.; Ravindran P. N. Direct shoot organogenesis from leaf explants of Embelia ribes Burm. f.: a vulnerable medicinal plant. J For Res 11: 57–60; 2006b.

    Article  Google Scholar 

  • Rajasri B.; Sabita B. In vitro multiplication of Coleus forskohlii Briq.: an approach towards shortening the protocol. In Vitro Cell Dev Biol Plant 37: 572–575; 2001.

    Google Scholar 

  • Rama Rao B.; Vijay Kumar D.; Amrutha R. N.; Jalaja N.; Vaidyanath K. Effect of growth regulators, carbon source and cell aggregate size on berberine production from cell cultures of Tinospora cordifolia Miers. Curr Trends Biotechnol Pharm 2: 269–276; 2008.

    Google Scholar 

  • Ramavat K. G.; Dass S.; Mathur N. The chemical diversity of bioactive molecules and therupetic potential of medicinal plants. In: Ramavat K. G. (ed) Herbal drugs: Ethnemedicine to Modern Medicine. Springer, Heidelberg, pp 7–32; 2009.

    Chapter  Google Scholar 

  • Ratnam W.; Teik N. L. Practical applications of biotechnology in conservation of medicinal and aromatic plants. In: Kamaruddin M. S.; Natesh S.; Osman A.; Azizol A. K. (eds) Medicinal and aromatic plants: strategies and technologies for conservation. Forest Research Institute, Kuala Lumpur, pp 53–62; 1999.

    Google Scholar 

  • Roja G.; Heble M. R. The quinoline alkaloids camptothecin and 9-methoxycamptothecin from tissue cultures and mature trees of Nothapodytes foetida. Phytochemistry 36: 65–66; 1994.

    Article  CAS  Google Scholar 

  • Roja G.; Heble M. R. Indole alkaloids in clonal propagules of Rauwolfia serpentina Benthe ex kurz. Plant Cell Tissue Organ Cult 44: 111–115; 1996.

    Article  CAS  Google Scholar 

  • Rubluo A.; Chavez V.; Martinez A. In vitro seed germination and reintroduction of Bletia urbana (Orchidaceae) in its natural habitat. Lindleyana 4: 68–73; 1989.

    Google Scholar 

  • Samarajeewa P. K.; Dassanayake M. D.; Jayawardena S. D. G. Clonal propagation of Gloriosa superba. Indian J Exp Biol 31: 719–720; 1993.

    CAS  Google Scholar 

  • Sarasan V.; Soniya E. V.; Nair G. M. Regeneration of Indian sarasaparilla, Hemidesmus indicus R.Br., through organogenesis and somatic embryogenesis. Indian J Exp Biol 32: 284–287; 1994.

    Google Scholar 

  • Sarker K. P.; Islam A.; Islam R.; Hoque A.; Joarder O. I. In vitro propagation of Rauvolfia serpentina through tissue culture. Planta Med 62: 358–359; 1996.

    Article  PubMed  CAS  Google Scholar 

  • Sasikumar S.; Raveendar S.; Premkumar A.; Ignacimuthu S.; Agastian P. Micropropagation of Baliospermum montanum (Willd)Muell. Arg. a threatened medicinal plant. Indian J Biotechnol 8: 223–226; 2009.

    CAS  Google Scholar 

  • Satheesh Kumar K.; Seeni S. In vitro multiplication of Nothopodites foetida (Wight) Sleumer through seedling explant cultures. Indian J Exp Biol 38: 273–277; 2000.

    CAS  Google Scholar 

  • Satheesh Kumar K.; Seeni S. Production of plumbagin (5-hydroxy-2methoxy 1, 4-naphtoquinone) in callus and cell suspension cultures of Plumbago indica Linn. Indian J Biotechnol 1: 305–308; 2002.

    CAS  Google Scholar 

  • Satheesh Kumar K.; Seeni S. In vitro mass multiplication and production of roots in Plumbago rosea. Planta Med 69: 83–86; 2003.

    Article  CAS  Google Scholar 

  • Satheesh G.; Geetha S. P.; Sudhakar R. S.; Indira B.; Ravindran P. N. In vitro medium-term conservation of Bacopa monnieri (L.) Pennell—the memory plus plant—under slow-growth conditions. PGR News Lett 151: 49–55; 2003.

    Google Scholar 

  • Sayeed Hassan A. K. M.; Roy K. S. Micropropagation of Gloriosa superba L. fhrough high frequency shoot proliferation. Plant Tissue Cult 15: 67–74; 2005.

    Google Scholar 

  • Seeni S.; Latha P. G. In vitro multiplication and eco-rehabilitation of the endangered Blue Vanda. Plant Cell Tissue Organ Cult 61: 1–8; 2000.

    Article  CAS  Google Scholar 

  • Sen J.; Sharma A. K.; Sahu N. P.; Mahato S. B. Production of forskolin in in vitro cultures of Coleus forskohlii. Planta Med 58: 324–327; 1992.

    Article  PubMed  CAS  Google Scholar 

  • Shahanaz Beegum A.; Martin K. P.; Zhang C. L.; Nishita I. K.; Ligimol S. A.; Madhusoodanan P. V. Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant. Electron J Biotechnol 10(1): 7; 2007.

    Google Scholar 

  • Shalaka D. K.; Sandhya P. Micropropagation and organogenesis in Adhatoda vasica for the estimation of vasine. Pharm Mag 5: 539–363; 2009.

    Google Scholar 

  • Sharad T.; Pankaj S.; Kanchan S. In vitro propagation of Pterocarpus marsupium Roxb.: an endangered medicinal tree. Indian J Biotechnol 3: 422–425; 2004.

    Google Scholar 

  • Sharma N.; Satsangi R.; Pandey R.; Vimala Devi S. In vitro clonal propagation and medium term conservation of brahmi (Bacopa monnieri (L) wettst). J Plant Biochem Biotechnol 16: 139–43; 2007.

    CAS  Google Scholar 

  • Shilpashree H. P.; Ravishankar R. In vitro plant regeneration and accumulation of flavanoids in Hypericum mysorense. Int J Integr Biol 8: 43–49; 2009.

    CAS  Google Scholar 

  • Shin S. Studies on the production of anthraquinone derivatives by tissue culture of Rubia species. Arch Pharm Res 12: 99–102; 1989.

    Article  Google Scholar 

  • Singh B.; Sood N. Significance of explant preparation and sizing in Aloe vera L.- A highly efficient method for in vitro multiple shoot induction. Sci Hortic 122: 146–151; 2009.

    Article  Google Scholar 

  • Sivakumar G.; Krishnamurthy K. V.; Hao J.; Paek K. Y. Colchicine production in Gloriosa superba calluses by feeding precursors. Chem Nat Compd 40: 499–502; 2004.

    Article  CAS  Google Scholar 

  • Soniya E. V.; Das M. R. In vitro micropropagation of Piper longum—an important medicinal plant. Plant Cell Tissue Organ Cult 70: 325–327; 2002.

    Article  CAS  Google Scholar 

  • Soniya E. V.; Sujitha M. An efficient in vitro propagation of Aristolochia indica. Biol Plant 50: 272–274; 2006.

    Article  Google Scholar 

  • Sreekumar S.; Seeni S.; Pushpangadan P. Production of 2-hydroxy 4-methoxy benzaldehyde using root cultures of Hemidesmus indicus R.Br. Biotechnol Lett 20: 631–635; 1998.

    Article  CAS  Google Scholar 

  • Sreekumar S.; Seeni S.; Pushpangadan P. Micropropagation of Hemidesmus indicus R.Br. for cultivation and production of 2-hydroxy 4-methoxy benzaldehyde. Plant Cell Tissue Organ Cult 62: 211–218; 2000.

    Article  CAS  Google Scholar 

  • Srinath R.; Govinda Raju B. Role of plant biotechnology in production of secondary metabolites. In: Irfan A. K.; Atiya Khan (eds) Role of biotechnology in medicinal and aromatic plants, Vol 2. Ukaaz, Hyderabad, pp 392–403; 1999.

    Google Scholar 

  • Stanly C.; Keng C. L. Micropropagation of Curcuma zedoaria Roscoe and Zingiber zerumbet Smith. Biotechnol 6: 555–560; 2007.

    Article  CAS  Google Scholar 

  • Sudha C. G.; Krishnan P. N.; Pushpangadan P. In vitro propagation of Holostemma annulare (Roxb.) Schum, a rare medicinal plant. In vitro Cell Dev Biol Plant 33: 57–63; 1998.

    Google Scholar 

  • Sudha C. G.; Krishnan P. N.; Pushpangadan P.; Seeni S. In vitro propagation of Decalepis arayalpathra, a critically endangered ethnomedicinal plant for ex-situ conservation. In vitro Cell Dev Biol Plant 41: 648–654; 2005.

    Article  Google Scholar 

  • Sudha C. G.; Krishnan P. N.; Seeni S.; Pushpangadan P. Regeneration of plants from in vitro root segments of Holostemma annulare (Roxb.) K.Schum., a rare medicinal tree. Curr Sci 78: 503–506; 2000.

    CAS  Google Scholar 

  • Sudha C. G.; Obul Reddy B.; Ravishankar G. A.; Seeni S. Production of ajmalicine in hairy root cultures of Rauvolfia micrantha Hook., a rare and endangered medicinal plant. Biotechnol Lett 25: 631–636; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Sudha C. G.; Seeni S. In vitro multiplication and field establishment of Adhatoda beddomei C.B. Clarke, a rare medicinal plant. Plant Cell Rep 13: 203–207; 1994.

    Article  CAS  Google Scholar 

  • Sudha C. G.; Seeni S. In vitro propagation of Rauvolfia micrantha, a rare medicinal plant. Plant Cell Tissue Organ Cult 44: 243–248; 1996.

    Article  Google Scholar 

  • Sudha C. G.; Seeni S. Establishment and analysis of fast-growing normal root culture of Decalepis arayalpathra, a rare endemic medicinal plant. Curr Sci 81: 371–374; 2001.

    CAS  Google Scholar 

  • Suja A. (2005) Medicinal plants of Western Ghats. In: Sahyadri E.News: Issue XII http://www.ces.iisc.ernet.in/biodiversity/sahyadri_enews/newsletter/issue12/Index.htm

  • Sundravelan R.; Desireddy B.; Ciddi V. Production of camptothecines from callus cultures of Nothapodytes foetida (Wight) Sleumer. Indian J Biotechnol 3: 452–453; 2004.

    CAS  Google Scholar 

  • Suzuki H.; Matsumoto T.; Mikami Y. Effects of physical factors and surface active agents on the formation of anthraquinones by Rubia cordifolia cells in suspension culture. Agric Biol Chem 49: 519–520; 1985.

    CAS  Google Scholar 

  • Swapna T. S.; Binitha M.; Manju T. S. In vitro multiplication in Kaempferia galanga Linn. Appl Biochem Biotechnol 118: 233–241; 2004.

    Article  PubMed  CAS  Google Scholar 

  • Thengane S. R.; Kulkarni D. K.; Shrikhande V. A.; Joshi S. P.; Sonawane K. B.; Krishnamurthy K. V. Influence of medium composition on callus induction and camptothecin(s) accumulation in Nothapodytes foetida. Plant Cell Tissue Organ Cult 72: 247–251; 2003.

    Article  CAS  Google Scholar 

  • Tyagi R. K.; Anuradha A.; Akkara Y. Conservation of Zingiber germplasm through in vitro rhizome formation. Sci Hortic 108: 210–219; 2006.

    Article  CAS  Google Scholar 

  • Tyagi R. K.; Yusuf A.; Dua P.; Agrawal A. In vitro Plant Regeneration and Genotype Conservation of Eight Wild Species of Curcuma. Biol Plant 48: 129–132; 2004.

    Article  CAS  Google Scholar 

  • Vanisree M.; Hsin-Sheng T. Plant cell cultures- an alternative and efficient source for the production of biologically important secondary metabolites. Int J Appl Sci Eng 1: 29–48; 2004.

    Google Scholar 

  • Verma P. C.; Singh D.; Rahman L.; Gupta M. M.; Banerjee S. In vitro studies in Plumbago zeylanica: rapid micropropagation and establishment of higher plumbagin yeilding hairy root cultures. J Plant Physiol 159: 547–552; 2002.

    Article  CAS  Google Scholar 

  • Vines G. (2004) Herbal harvests with a future: towards a sustainable source for medicinal plants, Plant life International.www.plantlife.org.uk

  • Wochok Z. S. The role of tissue culture in preserving threatened and endangered plant species. Biol Conserv 20: 83–89; 1981.

    Article  Google Scholar 

  • Yamamoto O.; Yamada Y. Production of reserpine and its optimization in cultured Rauwolfia serpentina Benth. cells. Plant Cell Rep 5: 50–53; 1986.

    Article  CAS  Google Scholar 

  • Yamuna G.; Babu N. K. Cryopreservation of black pepper somatic embryos. In: Proceedings of the 22nd Kerala Science Congress, Kerala State Council for Science, Technology and Environment. Thiruvananthapuram, Kerala, pp 241–242; 2010.

    Google Scholar 

  • Yamuna G.; Sumathi V.; Geetha S. P.; Praveen K.; Swapna N.; Nirmal Babu K. Cryopreservation of in vitro grown shoots of ginger (Zingiber officinale Rosc.). Cryoletters 28: 241–252; 2007.

    PubMed  CAS  Google Scholar 

  • Yogananth N.; Jothi Basu M. TLC method for the determination of plumbagin in hairy root culture of Plumbago rosea L. Glob J Biotechnol Biochem 4: 66–69; 2009.

    CAS  Google Scholar 

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Acknowledgements

The authors acknowledge the Director, TBGRI for providing facilities and encouragement and the Department of Biotechnology, Government of India for providing financial assistance.

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  1. Plant Biotechnology and Bioinformatics Division, Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, 695 562, India

    Peringattulli Narayanan Krishnan, S. W. Decruse & R. K. Radha

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Krishnan, P.N., Decruse, S.W. & Radha, R.K. Conservation of medicinal plants of Western Ghats, India and its sustainable utilization through in vitro technology. In Vitro Cell.Dev.Biol.-Plant 47, 110–122 (2011). https://doi.org/10.1007/s11627-011-9344-9

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