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Effect of phytohormones on micropropagation of Artemisia vulgaris L.

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Abstract

This paper describes an efficient in vitro micropropagation of Artemisia vulgaris using shoot tip and nodal explants. Among the various growth regulators tested, MS medium and B5 vitamins supplemented with BA (4.44 μM) and KN (2.32 μM) combination was found to yield a better response than BA (4.44–13.32 μM) or KN (0.46–13.92 μM) alone in the medium. BA and KN combinations produced a maximum of 23.3 shoots per explant with 99.8% shooting frequency. Multiple shoots raised were elongated on MS medium containing 0.44 μM BA and 1.44 μM GA3. Rooting was highest (98.2%) on MS medium containing 8.56 μM IAA. Rooted plantlets were successfully transferred to plastic cups containing autoclaved garden soil, farmyard soil and sand (2:1:1) for hardening. After 65 days, the plantlets were transferred to Botanical Evaluation Garden and maintained. The survival rate of plantlets varied under acclimatization. Plants looked healthy with no visually detectable phenotypic variations. This is the first report on plant regeneration via organogenesis of A. vulgaris.

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References

  • Anuradha M, Pullaiah T (1992) Micropropagation of mulberry (Morus alba L.). Annali Di Botanica 15:35–41

    Google Scholar 

  • Asima C, Satyesh CP (1997) The treatise of Indian medicinal plants. National Institute of Science Communication, New Delhi, pp 142–143

  • Baskaran P, Jayabalan N (2005) An efficient micropropagation system for Eclipta alba—a valuable medicinal herb. In vitro Cell Dev Biol Plant 41:532–539

    Article  CAS  Google Scholar 

  • Cheepala SB, Sharma NC, Sahi SV (2004) Rapid in vitro regeneration of Sesbania drummondii. Biol Plant 48(1):13–18

    Article  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  PubMed  CAS  Google Scholar 

  • Geng S, Ma M, Ye HC, Liu BY, Li GF, Cong K (2001) Effect of ipt gene expression on the physiological and chemical characteristics of Artemisia annua L. Plant Sci 160:691–698

    Article  CAS  Google Scholar 

  • George DP, Roger MD (2000) Encyclopedia of medicinal plants. Education and Health Library, Editoriael Safeliz, S.L. Publishers, Spain 2:624

  • George EF, Sherrington PF (1984) Plant propagation by tissue culture. Exegetics Ltd., Eversley, pp 39–71

    Google Scholar 

  • Gomez KA, Gomez KA (1976) Statistical procedures for agricultural research with emphasis on rice. Philippines International Rice Research Institute, Los Bans

    Google Scholar 

  • Hu CY, Wang PJ (1983) Meristem shoot tip and bud culture. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture. Macmillan, New York, pp 177–227

    Google Scholar 

  • Indhra DB, Dhar U (2000) Micropropagation of Indian wild strawberry. Plant Cell Tiss Org Cult 60:83–88

    Article  Google Scholar 

  • Jamison JA, Renfroe MH (1998) Micropropagation of Betula uber (Ashe) Fernold. In vitro Cell Dev Biol Plant 34:147–151

    Google Scholar 

  • Kapoor LD (2000) CRC Handbook of Ayurvedic medicinal plants. CRC Press, Boca Raton, p 53

  • Liu CZ, Murch SJ, El-Demerdash M, Saxena PK (2003) Regeneration of the Egyptian medicinal plant Artemisia judaica L. Plant Cell Rep 21:525–530

    PubMed  CAS  Google Scholar 

  • Mackay WA, Kitto SL (1988) Factors affecting in vitro shoot proliferation of French Tarragon. J Am Soc Hortic Sci 113:282–287

    CAS  Google Scholar 

  • Mathe A, Laszloffy K (1991) Data to the in vitro morphogenesis of Artemisia annua L. Acta Hortic 300:293–299

    Google Scholar 

  • Mneny EE, Mantell SH (2002) Clonal propagation of cashew (Anacardium occidentale L.) by tissue culture. J Hort Sci Biotech 77:649–657

    Google Scholar 

  • Mozetti C, De-Donato M (1998) Micropropagation of Artemisia mutellina Vill. Acta Hortic 457:257–259

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murch SJ, KrishnaRaj S, Saxena PK (2000) Phytomaceuticals: Mass production, standardization and conservation. Sci Rev Alternative Med 4:39–43

    Google Scholar 

  • Nair NG, Kartha KK, Gamborg OL (1979) Effect of growth regulators on plant regeneration from shoot tip meristems of cassava (Manihot esculenta Crantz) and the culture of internodes in vitro. Z Pflanzenphysiol 95:51–56

    CAS  Google Scholar 

  • Nin S, Morosi E, Schiffs S, Bennici A (1996) Callus cultures of Artemisia absinthium L.: initiation, growth, optimization and organogenesis. Plant Cell Tiss Org Cult 45:67–72

    Article  Google Scholar 

  • Pandey NK, Tewari KC, Tewari RN, Joshi GC, Pande VN, Pandey G (1993) Medicinal plants of Kumaon Himalaya: strategies for conservation. In: Dhar U (ed) Himalayan biodiversity conservation strategies. Himavikas Publications, Nainital, pp 293–302

    Google Scholar 

  • Parrot WA, Bailey MA, Durham RE, Mathews HV (1992) Tissue cultures and regeneration in legumes. In: Moss JP (ed) Biotechnology and crop improvement in Asia. ICRISAT, Patancheru, India, pp 115–148

  • Preece JE, Huttreman CA, Ashby WC, Roth PL (1991) Micro cutting propagation of silver maple. I. Results with adult and juvenile propagules. J Am Soc Hort Sci 116:142–148

    CAS  Google Scholar 

  • Ram PR, Mehrotra MN (1995) Compendium of Indian medicinal plants, publication and information directorate, CSIR, New Delhi, 4 (1985–1989):74

  • Saini R, Jaiwal PK (2000) In vitro multiplication of Peganum harmala, an important medicinal plant. Ind J Exp Biol 38:499–503

    CAS  Google Scholar 

  • Saxena PK (2001) Preface to special issue on in vitro culture of medicinal plants. Plant Cell Tiss Org Cult 62:167

    Google Scholar 

  • Shaik I, Hussain SJ (2004) Some important herbs used in the treatment of cancer: part I. Role of biotechnology in medicinal and aromatic plants. Ukaaz Publications, Hyderabad, 11:10–11

  • Shamsudeen Varisai M, Jawahar M, Thiruvengadam M, Jeyakumar M, Jayabalan N (1999) Effect of cytokinins on the proliferation of multiple shoots in horse gram (Macrotyloma uniflorum (Lam.) verdc.). J Plant Biotechnol 1(2):79–83

    Google Scholar 

  • Sharief MU, Jagadishchandra KS, Johnson TS, Ravishankar GA (1997) Propagation of Artemisia pallens by encapsulated in vitro grown shoot buds. J Med Arom Plant Sci 19:712–716

    Google Scholar 

  • Shiva Prakash N, Deepak P, Neera BS (1994) Regeneration of pigeon pea (Cajanus cajan) from cotyledonary node via multiple shoot formation. Plant Cell Rep 13:623–627

    Article  CAS  Google Scholar 

  • Taiz L, Zeiger E (1998) Plant Physiology. Sinauer Associates Inc., Masschusettes, p 792

    Google Scholar 

  • Tejavathi DH, Shailaja KS (1999) Regeneration of plants from the cultures of Bacopa monnieri (L.) Pennell. Phytomorphology 49(4):447–452

    Google Scholar 

  • Umer Sharief MD, Jagadish Chandra KS (1991) Micropropagation of Davana (Artemisia pallens wall) by tissue culture. In: Prakash J, Pierik RLM (eds) Horticulture—new technologies and applications. Kluwer, The Netherlands, pp 258–263

    Google Scholar 

  • USDA-ARS-NGRL (2004) Dr. Duke’s phytochemical and ethnobotanical databases. http://www.ars-grin.gov/duke/chem-activities.html

  • Venkatachalam P, Jayabalan N (1997) Effect of auxins and cytokinins on efficient regeneration and multiple shoot formation from cotyledons and cotyledonary node explants of groundnut (Arachis hypogaea L.) by in vitro culture technology. Appl Biochem Biotechnol 67:237–247

    CAS  Google Scholar 

  • Vijaya Kumar J, Ranjitha Kumari BD (2005) Effect of phytohormones on multiple shoot bud induction in cv. NARI-6 of safflower (Carthamus tinctorius L.). J Plant Biotechnol 7(3):149–153

    Google Scholar 

  • Vijayalakshmi G, Giri CC (2003) Plant regeneration via organogenesis from shoot base derived callus of Arachis stenosperma and A. villosa. Curr Sci 85(11):1624–1629

    Google Scholar 

  • Walter HL, Memory PF, Elvin-Lewis (2003) Medical botany. In: Plants affecting human health, 2nd edn. Wiley, New Jersey, p 345

    Google Scholar 

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Authors and Affiliations

  1. Stress Biology and Medicinal Plant Biotechnology Division, Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    G. Sujatha & B. D. Ranjitha Kumari

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  1. G. Sujatha
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  2. B. D. Ranjitha Kumari
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Correspondence to B. D. Ranjitha Kumari.

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Communicated by M. Saniewski.

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Sujatha, G., Kumari, B.D.R. Effect of phytohormones on micropropagation of Artemisia vulgaris L.. Acta Physiol Plant 29, 189–195 (2007). https://doi.org/10.1007/s11738-006-0023-0

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  • DOI: https://doi.org/10.1007/s11738-006-0023-0

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