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An efficient micropropagation protocol for an endangered tree species Aflatunia ulmifolia (Franch.) Vassilcz

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Abstract

Aflatunia ulmifolia (Franch.) Vassilcz. (Rosaceae) is an ornamental plant commonly used for landscaping and is known to form hybrids with other species that have characteristics like frost-resistant and non-rotting rootstocks. However, it is listed as an endangered species in Kazakhstan and is difficult to grow through traditional propagation; thus, in the present study, in vitro regeneration using seed and nodal explants was attempted. Murashige and Skoog (MS), Woody Plant Medium, and Gamborg medium supplemented with different plant growth regulators were utilized. Maximum germination (51.7±4.4%) was achieved by supplementing MS medium with 1.0 mg L-1 6-benzyladenine (BA) in which maximum shoot length (1.91±0.11 cm), leaves per shoot (8.48±0.32), and shoots per seed (1.83±0.15) were also observed after 12 wk. Comparatively, nodal explants proved better as 58.9±2.9% bud break was noted, inducing 2.84±0.18 shoots per explant with 0.74±0.01 cm length and 5.96±0.17 leaves per shoot at 1.0 mg L-1 BA after 8 wk. Nodal derived shoots were further multiplied on MS medium in the presence of 1.5 mg L-1 BA where 3.90±0.32 shoots (96.7% response) formed with 1.33±0.04 cm length after 4 wk. These shoots were rooted in MS media augmented with 0.5 mg L-1 indole-3-acetic acid, which induced 3.5±0.3 roots per shoot (83.3% response) with 3.8±0.1 cm root length at the end of 4 wk. The plantlets were then transferred to peat:vermiculite (1:1 w/w) for hardening and acclimatization and were finally transferred in the open ground with 69.4% survival rate after 1 mo. This micropropagation protocol will be helpful for the conservation of the genetic resources and biodiversity for A. ulmifolia.

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Acknowledgements

This work was supported by the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan under Grant No. AR09057922.

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Authors and Affiliations

  1. Department of Breeding, A.N. Bukeikhan Kazakh Research Institute of Forestry and Agroforestry, Shchuchinsk, Kazakhstan

    Vitaliy Kirillov, Mariya Serafimovich, Meirzhan Daulenova, Mikhail Gorbachenkov, Mussa Zholdasbayev & Tamara Stikhareva

  2. Department of Botany, University of Rajasthan, Jaipur, Rajasthan, India

    Ashutosh Pathak

  3. Department of Botany, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India

    Swati R. Patel

  4. School of Pharmacy, Karaganda Medical University, Karaganda, Kazakhstan

    Mussa Zholdasbayev

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  1. Vitaliy Kirillov
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Kirillov, V., Pathak, A., Patel, S.R. et al. An efficient micropropagation protocol for an endangered tree species Aflatunia ulmifolia (Franch.) Vassilcz. In Vitro Cell.Dev.Biol.-Plant 60, 28–38 (2024). https://doi.org/10.1007/s11627-023-10392-y

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