Abstract
A novel endophytic fungus producing beta-glucosidase was isolated and characterized from pigeon pea (Cajanus cajan [L.] Millsp.), which has excellent properties in converting ginsenoside Rb1 to ginsenoside Rd in Panax notoginseng. According to the 16S rDNA gene sequence, the G11-7 strain was identified as Fusarium proliferatum, and the accession number KY303906 was confirmed in GenBank. The G11-7 immobilized spores, in which the activity of beta-glucosidase could reach 0.95 U/mL, were co-cultured with P. notoginseng plant material to obtain a continuous beta-glucosidase supply for the biotransformation of ginsenoside Rb1 to Rd. Under the liquid–solid ratio (20:1), initial pH (6.0), and temperature (30 °C) constituents, the maximum ginsenoside Rd yield was obtained as 9.15 ± 0.65 mg/g, which was 3.67-fold higher than that without fungal spore co-culture (2.49 ± 0.98 mg/g). Furthermore, immobilized G11-7 spores showed significant beta-glucosidase producing ability which could be recovered and reused for 6 cycles. Overall, these results suggested that immobilized G11-7 offered a promising and effective approach to enhance the production of ginsenoside Rd for possible nutraceutical and pharmaceutical uses.
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This work was supported by the Fundamental Research Funds for the Central Universities (3332022052, 2572017AA08), the National Science Foundation of China (31930076, 31922058), and the Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team).
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LlN designed the experiment, contributed to its writing, corrected the manuscript, and prepared all figures. XyQ performed biotransformation experiments, analyzed data, and wrote the manuscript. LtW, NG, and HyC helped in the experimental accomplishment and corrected the manuscript. HhL performed the extraction of compounds. CjZ and HmW performed the identification of compounds. YjF supported finances, prepared plant materials, gave assistance to experiments, and corrected manuscript. All authors read and approved the final manuscript.
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Niu, L., Qin, X., Wang, L. et al. Upgrading the accumulation of ginsenoside Rd in Panax notoginseng by a novel glycosidase-producing endophytic fungus G11-7. Folia Microbiol 68, 441–452 (2023). https://doi.org/10.1007/s12223-022-01020-0
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DOI: https://doi.org/10.1007/s12223-022-01020-0