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Microbial transformation of ginsenoside Rb1 by Acremonium strictum

  • Applied Microbial and Cell Physiology
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Abstract

Preparative-scale fermentation of ginsenoside Rb1 (1) with Acremonium strictum AS 3.2058 gave three new compounds, 12β-hydroxydammar-3-one-20 (S)-O-β-d-glucopyranoside (7), 12β, 25-dihydroxydammar-(E)-20(22)-ene-3-O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranoside (8), and 12β, 20 (R), 25-trihydroxydammar-3-O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranoside (9), along with five known compounds, ginsenoside Rd (2), gypenoside XVII (3), ginsenoside Rg3 (4), ginsenoside F2 (5), and compound K (6). The structural elucidation of these metabolites was based primarily on one- and two-dimensional nuclear magnetic resonance and high-resolution electron spray ionization mass spectra analyses. Among these compounds, 2–6 are also the metabolites of ginsenoside Rb1 in mammals. This result demonstrated that microbial culture parallels mammalian metabolism; therefore, A. strictum might be a useful tool for generating mammalian metabolites of related analogs of ginsenosides for complete structural identification and for further use in pharmaceutical research in this series of compounds. In addition, the biotransformation kinetics was also investigated.

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Acknowledgment

This work was supported by Shanghai Commission of Science and Technology (0511021024) and National Supporting Program for Traditional Chinese Medicine from the Ministry of Science and Technology of China (2006BAI08B03-03).

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Authors and Affiliations

  1. College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, People’s Republic of China

    Guang-Tong Chen, Yan Song & Li-Jun Wu

  2. Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 199 Guo Shoujing Road, Zhangjiang, Shanghai, 201203, People’s Republic of China

    Guang-Tong Chen, Min Yang, Zhi-Qiang Lu, Jin-Qiang Zhang, Hui-Lian Huang & De-An Guo

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  1. Guang-Tong Chen
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  2. Min Yang
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  3. Yan Song
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  4. Zhi-Qiang Lu
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Correspondence to De-An Guo.

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Chen, GT., Yang, M., Song, Y. et al. Microbial transformation of ginsenoside Rb1 by Acremonium strictum . Appl Microbiol Biotechnol 77, 1345–1350 (2008). https://doi.org/10.1007/s00253-007-1258-4

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  • DOI: https://doi.org/10.1007/s00253-007-1258-4

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