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Assessment of the genetic polymorphism and physiological characterization of indigenous Oenococcus oeni strains isolated from Aglianico del Vulture red wine

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Abstract

The aim of this study was a reliable intra-species discrimination and strain biodiversity in Oenococcus oeni populations of two different Aglianico wineries by molecular, biochemical, and physiological characterization. Pulsed field gel electrophoresis (PFGE) analysis revealed a high polymorphism related to the origin (winery) of strains, while differential display PCR (DD-PCR) allowed a further discrimination of strains from the same winery. Moreover, the heterogeneity of these natural populations was investigated by capillary electrophoresis and enzymatic assays. A variability related to a different surface charge distribution was observed among strains, linked to their origin. Malolactic activity study evidenced strain-specific differences in malic acid degradation, and then, only the presence of L(-)-malic acid in the medium induced the mle gene. This study provided evidences on the importance of intra-species biodiversity of malolactic bacterial populations in wine ecosystems, as each wine possess peculiar winemaking conditions and physical–chemical properties which make specific the bacterial survival and growth. This study highlighted a great biodiversity among O. oeni strains that can be also winery specific. Such biodiversity within a certain winery and winemaking area is important for selecting malolactic starters, and strain-specific trait identification is especially important to match individual strains to specific industrial process.

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    Authors and Affiliations

    1. Dipartimento di Scienze, Università degli Studi della Basilicata, Viale dell’Ateneo Lucano 10, 85100, Potenza, Italy

      Caterina Cafaro, Maria Grazia Bonomo, Antonio Guerrieri, Fabiana Crispo, Rosanna Ciriello & Giovanni Salzano

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    1. Caterina Cafaro
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    2. Maria Grazia Bonomo
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    3. Antonio Guerrieri
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    4. Fabiana Crispo
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    5. Rosanna Ciriello
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    6. Giovanni Salzano
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    Correspondence to Maria Grazia Bonomo.

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    Caterina Cafaro and Maria Grazia Bonomo contributed equally to this work.

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    Cafaro, C., Bonomo, M.G., Guerrieri, A. et al. Assessment of the genetic polymorphism and physiological characterization of indigenous Oenococcus oeni strains isolated from Aglianico del Vulture red wine. Folia Microbiol 61, 1–10 (2016). https://doi.org/10.1007/s12223-015-0402-2

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