Abstract
Cytoplasmic male sterility (CMS) is an important trait for large-scale hybrid seed production which avoids manual emasculation and undesired horizontal spread of pollen. Rearrangements in mitochondrial genome in terms of deletions and insertions are frequent causes leading to CMS. Mitochondrial ATP synthase is a multisubunit molecular machine which is involved in synthesis of ATP. In this study, three mutations in ATPase subunit 6 were identified and their cosegregation with male sterility was established using tobacco male sterile hybrids and Nicotiana suvaolensis. A breeder friendly Kompetitive allele specific polymerase chain reaction (KASP) SNP marker was developed for high throughput and quick genotyping. Introgression of this trait into selected germplasm lines (\(n=9\)) was achieved based on foreground for CMS and background selection for recurrent parent using KASP marker and 50K custom tobacco SNP array, respectively. Analysis of genotyping data from SNP array revealed the presence of 88–99% of recurrent parent genome in BC\(_3\)F\(_1\) plants. The selected \(\hbox {BC}_{3}\hbox {F}_{1}\) plants exhibit CMS and are indistinguishable from the fertile recurrent parent (germplasm) in terms of plant morphology.
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Acknowledgements
Authors like to thank Dr C. C. Lakshmanan (Former) and Dr V. Krishnan (Present), Chief Scientist(s), Research & Technology Innovation, LSTC, ITC Limited and Mr Chitranjan Dar, member of CMC & Head of Quality Assurance, LSTC, CPO & EHS, ITC Limited for their encouragement and support. Authors would like to thank Dr D. V. N. Chakravarthy for help in arrangement of field experiments.
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Corresponding Editor: R. S. Sangwan
Priya B.N.V. is responsible for designing, conducting laboratory experiments and drafted the manuscript. Chidanand Ullagaddi and Dr Venkata Reddy were responsible for all the field related experiments. Dr Venkata Reddy played active role in conceptualization of the study, conducting SNP array experiments and edited the manuscript. Dr. Saiprasad guided, supervised the project and provided critical suggestions on manuscript preparation.
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Priya, B.N.V., Reddy, T.V., Chidanand, U. et al. Development of KASP marker for cytoplasmic male sterility in Nicotiana tabacum and utilization in trait introgression. J Genet 98, 79 (2019). https://doi.org/10.1007/s12041-019-1128-8
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DOI: https://doi.org/10.1007/s12041-019-1128-8