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Utility of Informative SSR Markers in the Molecular Characterization of Cytoplasmic Genetic Male Sterility-Based Hybrid and its Parents in Pigeonpea

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Abstract

Pigeonpea is one of the most important pulse crops in the semi-arid tropical region, which is prone to several climatic uncertainties like unpredictable temperature, frequent drought and inconsistent rainfall. Additionally, during crop cycle pigeonpea also encounters a wide range of other biotic and abiotic constraints, ultimately leading to its fluctuating production and stagnant productivity. However, recently developed CGMS system has shown noteworthy impacts in enhancing pigeonpea productivity through exploitation of hybrid vigour. At present, A2-cytoplasm derived CGMS system has been well established in pigeonpea. Nevertheless, the commercial success of CGMS system relies largely on the continuous supply of genetically pure seeds of hybrids and corresponding parental lines. Traditionally, the genetic purity of seeds is guaranteed through conducting grow out test (GoT). In this context, DNA marker assays offer several advantages over conventional GoT especially in terms of time, space and money. Given its locus-specific and co-dominant nature, SSR or microsatellite marker is particularly suited for hybridity testing and purity assessment. Here we report a set of robust SSR markers, which could act as reliable molecular kit for ensuring the genetic purity of the CGMS-hybrid ‘IPH 09-5’ and its parental lines ‘PA 163A’ (A-or Male sterile-line) and ‘AK 261322’ (R- or Restorer-line).

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Acknowledgments

Financial support from the Indian Council of Agricultural Research (ICAR) is gratefully acknowledged.

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Authors and Affiliations

  1. Crop Improvement Division, Indian Institute of Pulses Research (IIPR), Kanpur, 208024, India

    Abhishek Bohra, I. P. Singh, K. R. Soren, S. K. Chaturvedi & N. Nadarajan

  2. Division of Genetics, Indian Agricultural Research Institute (IARI), New Delhi, 110012, India

    Ashutosh K. Yadav

  3. National Bureau of Fish Genetic Resources, Lucknow, 226002, India

    Abhinav Pathak

  4. Biotechnology Unit, Indian Institute of Pulses Research (IIPR), Kanpur, 208024, India

    K. R. Soren

Authors
  1. Abhishek Bohra
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  2. I. P. Singh
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  3. Ashutosh K. Yadav
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  4. Abhinav Pathak
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  5. K. R. Soren
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  6. S. K. Chaturvedi
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  7. N. Nadarajan
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Correspondence to Abhishek Bohra.

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Bohra, A., Singh, I.P., Yadav, A.K. et al. Utility of Informative SSR Markers in the Molecular Characterization of Cytoplasmic Genetic Male Sterility-Based Hybrid and its Parents in Pigeonpea. Natl. Acad. Sci. Lett. 38, 13–19 (2015). https://doi.org/10.1007/s40009-014-0288-6

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  • DOI: https://doi.org/10.1007/s40009-014-0288-6

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