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Ameliorative Effects of Selenium on Cadmium-Induced Oxidative Stress and Endoplasmic Reticulum Stress in the Chicken Kidney

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Abstract

The harmful influences of dietary cadmium (Cd) on the chicken kidney and the protective role of selenium (Se) against Cd-induced nephrotoxicity in the chicken are relatively unexplored subjects. The aim of this study was to investigate the ameliorative role of Se on the effects of Cd-induced oxidative stress, endoplasmic reticulum stress, and apoptosis in chicken kidneys. For this study, 100-day-old chickens received Se (as 10 mg Na2SeO3/kg dry weight of diet), Cd (as 150 mg CdCl2/kg dry weight of diet), or Cd + Se in their diets for 60 days. Then, the histopathological changes, Cd and Se contents, levels of oxidative stress, inducible nitric oxide synthase-nitric oxide (iNOS-NO) system activity, levels of endoplasmic reticulum (ER) stress, results of the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay of apoptosis, and expression levels of Bcl-2 and caspase 3 in the kidney were examined. The results showed that Cd exposure caused histopathological and ultrastructural damage and apoptosis of the kidneys. Cd administration significantly increased the accumulation of Cd, the malondialdehyde (MDA) content, NO production, iNOS activity, iNOS expression levels, expression levels of ER stress-related genes (GRP78, GRP94, ATF4, ATF6, and IRE) and the pro-apoptosis gene caspase 3, and the rate of apoptosis. Cd administration markedly decreased the Se content, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, and anti-apoptosis gene Bcl-2 expression levels. Co-treatment with Se and Cd obviously reduced the accumulation of Cd, Cd-induced histopathological and ultrastructural changes, oxidative stress, iNOS-NO system activity, ER stress, caspase 3 expression levels, and the rate of apoptosis in the kidneys. These results suggested that Cd exposure caused renal injury and that Se ameliorated Cd-induced nephrotoxicity in chickens.

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Acknowledgments

The authors thank the members of the veterinary internal medicine laboratory in the College of Veterinary Medicine, Northeast Agricultural University for their help in collecting the kidney tissue samples. The authors thank the Elsevier English Language Editing System to correct grammatical, spelling, and other common errors.

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All authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, 150040, People’s Republic of China

    Lili Liu, Bingyou Yang & Yupeng Cheng

  2. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Lili Liu

  3. College of Applied Technology, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Hongjin Lin

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  1. Lili Liu
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  2. Bingyou Yang
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Correspondence to Hongjin Lin.

Additional information

Lili Liu holds a PhD degree, Heilongjiang University of Chinese Medicine, Northeast Agricultural University.

Bingyou Yang holds a PhD degree, Heilongjiang University of Chinese Medicine.

Yupeng Cheng holds a PhD degree, Heilongjiang University of Chinese Medicine.

Hongjin Lin holds a Ph.D degree, Northeast Agricultural University.

All other authors have read the manuscript and have agreed to submit it in its current form for consideration for publication in the journal.

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Liu, L., Yang, B., Cheng, Y. et al. Ameliorative Effects of Selenium on Cadmium-Induced Oxidative Stress and Endoplasmic Reticulum Stress in the Chicken Kidney. Biol Trace Elem Res 167, 308–319 (2015). https://doi.org/10.1007/s12011-015-0314-7

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