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The Effects of Flaxseed Oil on Cadmium-Induced Oxidative Stress in Rats

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Abstract

In the present study, the effects of flaxseed oil on the oxidant–antioxidant system in cadmium intoxication were investigated in rats. Forty-eight male Wistar albino rats were divided into four equal groups (group 1). No treatment was applied to the control group. On the other hand, groups 2, 3, and 4 were administered with 0.1 ml/rat/day (∼500 mg/kg bw) flaxseed oil by gavage into the stomach, 50 ppm of cadmium (∼4 mg/kg bw) in ad libitum drinking water, and 0.1 ml/rat/day flaxseed oil plus 50 ppm of cadmium, respectively, for 30 days. At the end of the study, malondialdehyde and nitric oxide levels and catalase, superoxide dismutase, and glutathione peroxidase activities were measured in blood and tissue (liver, lung, kidney, brain, heart, and testes) samples. While malondialdehyde and nitric oxide levels increased in the group given cadmium compared to the control group; in the meantime, there were some significant changes in antioxidant enzyme activities. These changes were observed, the trends of decrease or increase compared to the control group. There were positive changes in parameters of the group given with flaxseed oil plus cadmium compared to the group receiving cadmium alone, in other words, values were seen coming close to control group. As a result, cadmium exposure caused oxidative damage to erythrocytes and organs at varying rates, while flaxseed oil reduced the severity of cadmium-induced lipid peroxidation. Therefore, it was concluded that flaxseed oil can be used among compounds as a therapeutic agent or food additive for prophylaxis in cadmium intoxication.

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References

  1. Rubilar M, Gutiérrez C, Verdugo M, Shene C, Sineiro J (2010) Flaxseed as a source of functional ingredients. J Soil Sci Plant Nutr 10:373–377

    Article  Google Scholar 

  2. Yang W, Fu J, Yu M, Huang Q, Wang D, Xu J, Deng Q, Yao P, Huang F, Liu L (2012) Effects of flaxseed oil on antioxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level. Lipids Health Dis 11:88

    Article  PubMed  CAS  Google Scholar 

  3. Kasote DM (2013) Flaxseed phenolics as natural antioxidants. IFRJ 20:27–34

    CAS  Google Scholar 

  4. Touré A, Xueming X (2010) Flaxseed lignans: source, biosynthesis, metabolism, antioxidant activity, bioactive components, and health benefits. Compr Rev Food Sci F 9:261–269

    Article  Google Scholar 

  5. Nazıroğlu M (2011) TRPM2 cation channels, oxidative stress, and neurological diseases: where are we now? Neurochem Res 36:355–366

    Article  PubMed  Google Scholar 

  6. Somogyi A, Rosta K, Pusztai P, Tulassay Z, Nagy G (2007) Antioxidant measurements. Physiol Meas 28:R41–55

    Article  PubMed  Google Scholar 

  7. Guéraud F, Atalay M, Bresgen N, Cipak A, Eckl PM, Huc L, Jouanin I, Siems W, Uchida K (2010) Chemistry and biochemistry of lipid peroxidation products. Free Radic Res 44:1098–1124

    Article  PubMed  Google Scholar 

  8. Dailiah Roopha P, Padmalatha C (2012) Effect of herbal preparation on heavy metal (cadmium)-induced antioxidant system in female Wistar rats. J Med Toxicol 8:101–107

    Article  PubMed  CAS  Google Scholar 

  9. Lazarus M, Orct T, Aladrović J, Ljubić BB, Jurasović J, Blanuša M (2011) Effect of selenium pretreatment on antioxidative enzymes and lipid peroxidation in Cd-exposed suckling rats. Biol Trace Elem Res 142:611–622

    Article  PubMed  CAS  Google Scholar 

  10. Ognjanović BI, Marković SD, Pavlović SZ, Zikić RV, Stajn AS, Saicić ZS (2008) Effect of chronic cadmium exposure on antioxidant defense system in some tissues of rats: protective effect of selenium. Physiol Res 57:403–411

    PubMed  Google Scholar 

  11. Messaoudi I, El Heni J, Hammouda F, Saïd K, Kerkeni A (2009) Protective effects of selenium, zinc, or their combination on cadmium-induced oxidative stress in rat kidney. Biol Trace Elem Res 130:152–161

    Article  PubMed  CAS  Google Scholar 

  12. Cuypers A, Plusquin M, Remans T, Jozefczak M, Keunen E, Gielen H, Opdenakker K, Nair AR, Munters E, Artois TJ, Nawrot T, Vangronsveld J, Smeets K (2010) Cadmium stress: an oxidative challenge. Biometals 23:927–940

    Article  PubMed  CAS  Google Scholar 

  13. Nair AR, Degheselle O, Smeets K, Van Kerkhove E, Cuypers A (2013) Cadmium-induced pathologies: where is the oxidative balance lost (or not)? Int J Mol Sci 14:6116–6143

    Article  PubMed  CAS  Google Scholar 

  14. Dorta DJ, Leite S, DeMarco KC, Prado IM, Rodrigues T, Mingatto FE, Uyemura SA, Santos AC, Curti C (2003) A proposed sequence of events for cadmium-induced mitochondrial impairment. J Inorg Biochem 97:251–257

    Article  PubMed  CAS  Google Scholar 

  15. Flora SJ, Mittal M, Mehta A (2008) Heavy metal-induced oxidative stress and its possible reversal by chelation therapy. Indian J Med Res 128:501–523

    PubMed  CAS  Google Scholar 

  16. Sears ME (2013) Chelation: Harnessing and enhancing heavy metal detoxification-a review. Thescientificworldjo 219840

  17. Abdel Moneim AE, Dkhil MA, Al-Quraishy S (2011) The protective effect of flaxseed oil on lead acetate-induced renal toxicity in rats. J Hazard Mater 194:250–255

    Article  PubMed  CAS  Google Scholar 

  18. Abdel Moneim AE, Dkhil MA, Al-Quraishy S (2011) Effects of flaxseed oil on lead acetate-induced neurotoxicity in rats. Biol Trace Elem Res 144:904–913

    Article  PubMed  CAS  Google Scholar 

  19. Abdel-Moneim AE, Dkhil MA, Al-Quraishy S (2011) The redox status in rats treated with flaxseed oil and lead-induced hepatotoxicity. Biol Trace Elem Res 143:457–467

    Article  PubMed  CAS  Google Scholar 

  20. Abdel Moneim AE (2012) Flaxseed oil as a neuroprotective agent on lead acetate-induced monoamineric alterations and neurotoxicity in rats. Biol Trace Elem Res 148:363–370

    Article  PubMed  CAS  Google Scholar 

  21. Rizwan S, Naqshbandi A, Khan F (2013) Dietary flaxseed oil supplementation mitigates the effect of lead on the enzymes of carbohydrate metabolism, brush border membrane, and oxidative stress in rat kidney tissues. Biol Trace Elem Res 153:279–290

    Article  PubMed  CAS  Google Scholar 

  22. Winterbourn CC, Hawkins RE, Brian M, Carrell RW (1975) The estimation of red cell superoxide activity. J Lab Clin Med 55:337–341

    Google Scholar 

  23. Fairbanks VF, Klee GG (1987) Biochemical aspect of hematology. In: Tietz NW (ed) Fundamentals of Clinical Chemistry, 3rd edn. WB Saunders, Philadelphia, pp 803–806

    Google Scholar 

  24. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  25. Miller GL (1959) Protein determination for large numbers of samples. Anal Chem 31:964

    Article  CAS  Google Scholar 

  26. Yoshioka T, Kawada K, Shimada T, Mori M (1979) Lipid peroxidation in maternal and cord blood and protective mechanism against activated oxygen toxicity in the blood. Am J Obstet Gynecol 135:372–376

    PubMed  CAS  Google Scholar 

  27. Ohkawa H, Ohishi N, Yagi K (1978) Reaction of linoleic acid hydroperoxide with thiobarbutiric acid. J Lipid Res 19:1053–1057

    PubMed  CAS  Google Scholar 

  28. Luck H (1965) Catalase. In: Bergmeyer H (ed) Methods of Enzymatic Analysis. Academic Press, New York, pp 885–894

    Chapter  Google Scholar 

  29. Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497–500

    PubMed  CAS  Google Scholar 

  30. Paglia PE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169

    PubMed  CAS  Google Scholar 

  31. Tracey WR, Tse J, Carter G (1995) Lipopolysaccharide-induced changes in plasma nitrite and nitrate concentrations in rats and mice: pharmacological evaluation of nitric oxide synthase inhibitors. J Pharmacol Exp Ther 272:1011–1015

    PubMed  CAS  Google Scholar 

  32. Jomova K, Valko M (2011) Advances in metal-induced oxidative stress and human disease. Toxicology 283:65–87

    Article  PubMed  CAS  Google Scholar 

  33. Thévenod F (2009) Cadmium and cellular signaling cascades: to be or not to be? Toxicol Appl Pharmacol 238:221–239

    Article  PubMed  Google Scholar 

  34. Xu B, Chen S, Luo Y, Chen Z, Liu L, Zhou H, Chen W, Shen T, Han X, Chen L, Huang S (2011) Calcium signaling is involved in cadmium-induced neuronal apoptosis via induction of reactive oxygen species and activation of MAPK/mTOR network. PLoS One 6:e19052

    Article  PubMed  CAS  Google Scholar 

  35. Garcia JJ, Martínez-Ballarín E, Millán-Plano S, Allué JL, Albendea C, Fuentes L, Escanero JF (2005) Effects of trace elements on membrane fluidity. J Trace Elem Med Biol 19:19–22

    Article  PubMed  CAS  Google Scholar 

  36. El-Beltagi HS, Salama ZA, El-Hariri DM (2007) Evaluation of fatty acids profile and the content of some secondary metabolites in seeds of different flax cultivars (Linum Usitatissimum L.). Gen Appl Plant Physiology 33:187–202

    CAS  Google Scholar 

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Acknowledgments

This research (project code: TSY-09-753) was supported by the Research Fund of Erciyes University.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. College of Health Science, Department of Pharmacology and Toxicology, Erciyes University, Kayseri, Turkey

    Sedat Karaca

  2. Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Erciyes University, Kayseri, Turkey

    Gökhan Eraslan

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  1. Sedat Karaca
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  2. Gökhan Eraslan
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Corresponding author

Correspondence to Gökhan Eraslan.

Additional information

This study is the MSc thesis of Sedat Karaca.

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Karaca, S., Eraslan, G. The Effects of Flaxseed Oil on Cadmium-Induced Oxidative Stress in Rats. Biol Trace Elem Res 155, 423–430 (2013). https://doi.org/10.1007/s12011-013-9804-7

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  • DOI: https://doi.org/10.1007/s12011-013-9804-7

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