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Enzyme-Catalyzed Production of FAME by Hydroesterification of Soybean Oil Using the Novel Soluble Lipase NS 40116

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Abstract

The performance of lipase NS 40116, a novel and promising soluble enzyme obtained from modified Thermomyces lanuginosus microorganism, was investigated in the production of biodiesel (fatty acid methyl esters—FAME) by hydroesterification. In order to investigate the potential of the biocatalyst in its soluble form, this work reports the effect of water content and enzyme load, as well as the recovery and reuse of the biocatalyst. A FAME yield of 94.30% after 12 h was achieved at 35 °C by combining 0.50 wt% of lipase, 15 wt% of water, and a methanol:oil molar ratio of 4.5:1. The analysis of the time course reaction suggests that the triacylglycerides (TAGs) are hydrolyzed by the enzyme in a first step, generating free fatty acids (FFAs), followed by the esterification of these FFAs into FAME. In relation to the reusability assays, the lipase kept approximately 90% of its catalytic activity after five cycles of reuse. In this context, the findings of this study demonstrate that lipase NS 40116 can efficiently catalyze hydroesterification reactions under mild conditions, arising as a competitive alternative for biodiesel synthesis.

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Abbreviations

AV:

Acid value

FTIR:

Fourier transform infrared

FAME:

Fatty acid methyl esters

FFA:

Free fatty acids

KOH:

Potassium hydroxide

TAG:

Triacylglycerides

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Funding

This work received financial support from the National Council of Technological and Scientific Development (CNPq) and the Research Support Foundation of the State of Rio Grande do Sul (FAPERGS) throughout this research, as well the Coordination for the Improvement of Higher Education Personnel (CAPES) for scholarships.

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Authors and Affiliations

  1. Department of Chemical Engineering, Federal University of Santa Maria, 1000, Roraima Avenue, Santa Maria, 97105-900, Brazil

    Daniela V. Rosset, João H. C. Wancura, Raquel C. Kuhn & Sérgio L. Jahn

  2. Department of Crop Protection, Federal University of Santa Maria, 1000, Roraima Avenue, Santa Maria, 97105-900, Brazil

    Gustavo A. Ugalde

  3. Department of Chemical and Food Engineering, Federal University of Santa Catarina, Florianopolis, SC, 88040-900, Brazil

    J. Vladimir Oliveira

  4. Laboratory of Agroindustrial Processes Engineering (LAPE), Federal University of Santa Maria, 1040, Sete de Setembro St., Center DC, Cachoeira do Sul, RS, 96508-010, Brazil

    Marcus V. Tres

Authors
  1. Daniela V. Rosset
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  2. João H. C. Wancura
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  3. Gustavo A. Ugalde
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  4. J. Vladimir Oliveira
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  7. Sérgio L. Jahn
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Correspondence to Marcus V. Tres.

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Rosset, D.V., Wancura, J.H.C., Ugalde, G.A. et al. Enzyme-Catalyzed Production of FAME by Hydroesterification of Soybean Oil Using the Novel Soluble Lipase NS 40116. Appl Biochem Biotechnol 188, 914–926 (2019). https://doi.org/10.1007/s12010-019-02966-7

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