Abstract
Looking back at the literature for enzymatic biodiesel, it is evident that the research has been focused on using immobilized lipase to enable re-use of the enzyme due to price constraints on lipases used for catalyzing the transesterification process. The use of liquid formulations of lipase for biodiesel has recently been implemented in the industry. Technology for using liquid formulated lipases for enzymatic biodiesel production is new and, since enzyme prices have been reduced, it is now possible to simplify the process considerably and apply it for very low-quality oils. In this paper, the use of liquid lipase formulations for enzymatic biodiesel will be described along with a general proposal for an industrial-scale enzymatic biodiesel process with >95 % yield.
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Notes
ASTM standard D6751 United States.
AOCS Ca 5a-40. Free fatty acids.
AOCS Ck 2-09 standard method for method for the analysis of methanol content, cloud point, monoglyceride, total and free glycerin in biodiesel.
References
Xuebin Z, Feng Q, Chongli Y, Wei D, Dehua L (2015) Lipase-catalyzed process for biodiesel production: enzyme immobilization, process simulation and optimization. Renew Sustain Energy Rev 44:182–197
Fjerbaek L, Christensen KV, Norddahl B (2009) A review of the current state of biodiesel production using enzymatic transesterification. Biotechnol Bioeng 102:1298–1315
Nielsen PM, Brask J, Fjerbaek L (2008) Enzymatic biodiesel production: technical and economical considerations. Eur J Lipid Sci Technol 110:692–700
Xu Y, Nordblad M, Nielsen PM, Brask J, Woodley JM (2011) In situ visualization and effect of glycerol in lipase-catalyzed ethanolysis of rapeseed oil. J Mol Catal B Enzym 72:213–219
Poppe JK, Fernandez-Lafuente R, Rodrigues RC, Ayub MAZ (2015) Enzymatic reactors for biodiesel synthesis: present status and future prospects. Biotech Adv 33:511–525
Xu Y, Nordblad M, Nielsen PM, Brask J, Woodly JM (2011) In situ visualization and effect of glycerol in lipase-catalyzed ethanolysis of rapeseed oil. J Mol Catal B Enzym 72:213–219
Cesarini S, Diaz P, Nielsen PM (2013) Short communication: exploring a new, soluble lipase for FAMEs production in water-containing systems using crude soybean oil as a feedstock. Process Biochem 48:484–487
Lv D, Du W, Zhang G, Liu D (2010) Mechanism study on NS81006-mediated methanolysis of triglyceride in oil/water biphasic system for biodiesel production. Process Biochem 45:446–450
Kaied M, Samukawa T, Kondo A, Fukuda H (2001) Effect of methanol and water contents on production of biodiesel fuel from plant oil catalyzed by various lipases in a solvent-free system. J Biosci Bioeng 91:12–15
Ren H, Li Y, Du W, Liu D (2013) Free lipase-catalyzed esterification of oleic acid for fatty acid ethyl ester preparation with response surface optimization. J Am Oil Chem Soc 90:73–79
Nordblad M, Silva VTL, Woodley JM, Nielsen PM (2014) Identification of critical parameters in liquid enzyme-catalyzed biodiesel production. Biotechnol Bioeng 111(12):2446–2453
Pedersen AT, Nordblad M, Nielsen PM, Woodley JM (2014) Batch production of faee-biodiesel using a liquid lipase formulation. J Mol Catal B Enzym 105:89–94
Austin G, Burton R, Fan X (2012) Patent application WO 2012/106701. Fatty Acid Esterification Process
Price J, Hofmann B, Silva VTL, Nordblad M, Woodley JM, Huusom JK (2014) Mechanistic modelling of biodiesel production using a liquid lipase formulation. Biotechnol Prog 30:1277–1290
Price J, Nordblad M, Martel H, Chrabas B, Wang H, Nielsen PM, Woodley J (2015) Scale-up of industrial biodiesel production to 40 m3 using a liquid lipase formulation. Biotech Bioeng (accepted. ID 1510-61)
Rancke-Madsen A, Nielsen PM. Pat. WO2015/181308. Production of fatty acid alkyl esters with caustic treatment
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Nielsen, P.M., Rancke-Madsen, A., Holm, H.C. et al. Production of Biodiesel Using Liquid Lipase Formulations. J Am Oil Chem Soc 93, 905–910 (2016). https://doi.org/10.1007/s11746-016-2843-4
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DOI: https://doi.org/10.1007/s11746-016-2843-4