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Preparation of Biodiesel with Liquid Synergetic Lipases from Rapeseed Oil Deodorizer Distillate

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Abstract

To reduce industrial production cost, cheap and easily available rapeseed oil deodorizer distillates were used as feedstock to prepare biodiesel in this study. As a result, liquid forms of Candida rugosa lipase and Rhizopus oryzae lipase (ROL) were functioned as new and effective catalysts with biodiesel yield of 92.63% for 30 h and 94.36% for 9 h, respectively. Furthermore, the synergetic effect between the two lipases was employed to enhance biodiesel yield with a result of 98.16% in 6 h under optimized conditions via response surface methodology. The obtained conversion rate surpassed both yields of the individual two lipases and markedly shortened the reaction time. The resultant optimal conditions were ROL ratio 0.84, water content 46 wt% (w/w), reaction temperature 34 °C, and reaction time 6 h.

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Abbreviations

RODDs:

Rapeseed oil deodorizer distillates

CRL:

Candida rugosa lipase

ROL:

Rhizopus oryzae lipase

RSM:

Response surface methodology

FAME:

Fatty acid methyl ester

FAs:

Fatty acids

FFA:

Free fatty acid

TG:

Triglyceride

MD:

Yeast nutrient media minimal dextrose

BMGY:

Buffered glycerol-complex medium

YPD:

Yeast extract-peptone-dextrose

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (No. 31170078), the National High Technology Research and Development Program of China (No 2013AA065805), the Natural Science Foundation of Hubei Province (No. 2015CFA085), and the Fundamental Research Funds for HUST (Nos. 2014NY007 and 2014QN119). The authors thank Ms. Chen Hong, from the Centre of Analysis and Test, Huazhong University of Science and Technology for biodiesel analysis.

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Authors and Affiliations

  1. Key Laboratory of Molecular Biophysics of the Ministry of Education, China-EU Institute for Clean and Renewable Energy, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China

    Leping Zeng

  2. Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China

    Yaojia He, Liangcheng Jiao, Kai Li & Yunjun Yan

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  1. Leping Zeng
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Correspondence to Yunjun Yan.

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Zeng, L., He, Y., Jiao, L. et al. Preparation of Biodiesel with Liquid Synergetic Lipases from Rapeseed Oil Deodorizer Distillate. Appl Biochem Biotechnol 183, 778–791 (2017). https://doi.org/10.1007/s12010-017-2463-y

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