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Transesterification of Waste Frying Oil and Soybean Oil by Combi-lipases Under Ultrasound-Assisted Reactions

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Abstract

This work describes the use of an ultrasound system for the enzymatic transesterification of oils using combi-lipases as biocatalyst. The reactions were carried out evaluating the individual use of waste oil and fresh soybean oil, and the immobilized lipases CALB, TLL, and RML were used as biocatalysts. It was performed in a mixture design of three factors to obtain the ideal mixture of lipases according to the composition of fatty acids present in each oil, and the main reaction variables were optimized. After 18 h of reaction, ultrasound provided a biodiesel yield of about 90% when using soybean oil and 70% using the waste oil. The results showed that ultrasound technology, in combination with the application of enzyme mixtures, known as combi-lipases, and the use of waste oil, could be a promising route to reduce the overall process costs of enzymatic production of biodiesel.

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Acknowledgements

The authors would like to thank Mr. Ramiro Martinez (Novozymes, Spain S.A.) for kindly supplying the enzymes used in this research.

Funding

This work was supported by grants from the Brazilian Coordenação de Aperfoiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, ZC 91501-970, Brazil

    Jakeline Kathiele Poppe, Carla Roberta Matte, Rafael C. Rodrigues & Marco Antônio Záchia Ayub

  2. Department of Biocatalysis, ICP-CSIC, Campus UAM-CSIC, Cantoblanco, ZC 28049, Madrid, Spain

    Roberto Fernandez-Lafuente

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  1. Jakeline Kathiele Poppe
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  2. Carla Roberta Matte
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  4. Rafael C. Rodrigues
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  5. Marco Antônio Záchia Ayub
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Correspondence to Marco Antônio Záchia Ayub.

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Poppe, J.K., Matte, C.R., Fernandez-Lafuente, R. et al. Transesterification of Waste Frying Oil and Soybean Oil by Combi-lipases Under Ultrasound-Assisted Reactions. Appl Biochem Biotechnol 186, 576–589 (2018). https://doi.org/10.1007/s12010-018-2763-x

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  • DOI: https://doi.org/10.1007/s12010-018-2763-x

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