Abstract
Arthrospira platensis is rich in branched-chain amino acids (BCAAs) and serves as a promising alternative material for BCAA-containing peptide production. Herein, Arthrospira platensis whole protein was employed for BCAA-containing peptide preparation by determining BCAA contents of whole protein and C-phycocyanin and allophycocyanin subunits. Released peptidome profiles by different types of proteases were significantly gathered into three clusters through principal component analysis, with only 39 shared sequences in the identified peptidomes, in which the portfolio by papain contained the highest number of BCAA-containing peptides, accounting for a proportion over 95%. A portfolio of 569 peptides with differential abundances among the peptidomes by papain with increased hydrolysis time showed a dynamic change trend, corresponding to the degradation of long peptides into oligopeptides. Therefore, BCAA-containing peptides released from Arthrospira platensis depended on hydrolysis conditions, and changes in BCAA-containing peptidomes were monitored through a peptidomics workflow, contributing to the quality control of target peptides.
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Data will be made available on request.
Abbreviations
- BCAA:
-
Branched-chain amino acid
- FDR:
-
False discovery rate
- ANOVA:
-
Analysis of variance
- C-PC:
-
C-phycocyanin
- APC:
-
Allophycocyanin
- PCA:
-
Principal component analysis
- PC:
-
Principal component
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Funding
This work was supported by National Natural Science Foundation of China (32272262) and Youth Innovation Technology Project of Higher School in Shandong Province (2021KJ090).
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Yinuo Guo: methodology, investigation, data curation, formal analysis, and writing—original draft. Linrong Wu: investigation and data curation. Zihao Zhu: formal analysis and data curation. Hu Hou: writing—review and editing. Yanchao Wang: conceptualization, methodology, formal analysis, funding acquisition, supervision, project administration, and writing—review and editing.
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Highlights
• Target peptide with branched-chain amino acid is prepared from Arthrospira platensis.
• Whole protein is more suitable for target peptide production than primary components.
• Change of peptidome during hydrolysis is monitored through a peptidomics workflow.
• The peptidome by papain contains more target peptides with a proportion over 95%.
• A portfolio of 569 peptides with differential abundances changed with increased time.
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Guo, Y., Wu, L., Zhu, Z. et al. Investigation of Peptides Containing Branched-Chain Amino Acids from Arthrospira platensis Through A Peptidomics Workflow. Food Bioprocess Technol 17, 516–527 (2024). https://doi.org/10.1007/s11947-023-03147-5
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DOI: https://doi.org/10.1007/s11947-023-03147-5