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Exploring the Molecular Aetiology of Preeclampsia by Massive Parallel Sequencing of DNA

  • Preeclampsia (VD Garovic, Section Editor)
  • Published:
Current Hypertension Reports Aims and scope Submit manuscript

Abstract

Purpose of Review

This manuscript aims to review (for the first time) studies describing NGS sequencing of preeclampsia (PE) women’s DNA.

Recent Findings

Describing markers for the early detection of PE is an essential task because, although associated molecular dysfunction begins early on during pregnancy, the disease’s clinical signs usually appear late in pregnancy. Although several biochemical biomarkers have been proposed, their use in clinical environments is still limited, thereby encouraging research into PE’s genetic origin. Hundreds of genes involved in numerous implantation- and placentation-related biological processes may be coherent candidates for PE aetiology. Next-generation sequencing (NGS) offers new technical possibilities for PE studying, as it enables large genomic regions to be analysed at affordable cost. This technique has facilitated the description of genes contributing to the molecular origin of a significant amount of monogenic and complex diseases. Regarding PE, NGS of DNA has been used in familial and isolated cases, thereby enabling new genes potentially related to the phenotype to be proposed.

Summary

For a better understanding of NGS, technical aspects, applications and limitations are presented initially. Thereafter, NGS studies of DNA in familial and non-familial cases are described, including pitfalls and positive findings. The information given here should enable scientists and clinicians to analyse and design new studies permitting the identification of novel clinically useful molecular PE markers.

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Funding

This study and Laissue’s Lab were supported by El Rosario University (grant: CS/ABN062/GENIUROS 018-019). Colciencias also supported the present work (Grant: 831-2017, 122277758201).

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  1. Biopas Laboratoires, Biopas Group, Bogotá, Colombia

    Paul Laissue

  2. Inserm U1016, CNRS UMR8104, Institut Cochin, équipe FGTB, 24, rue du faubourg Saint-Jacques, 75014, Paris, France

    Paul Laissue & Daniel Vaiman

  3. CIGGUR Genetics Group, School of Medicine and Health Sciences, El Rosario University, Bogotá, Colombia

    Paul Laissue

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Paul Laissue declares that his present salary is paid by BIOPAS Laboratories for working in projects different to that described in the present manuscript. Daniel Vaiman declares that he has no conflict of interest.

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Laissue, P., Vaiman, D. Exploring the Molecular Aetiology of Preeclampsia by Massive Parallel Sequencing of DNA. Curr Hypertens Rep 22, 31 (2020). https://doi.org/10.1007/s11906-020-01039-z

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