Abstract
Blackberry (Rubus fruticosus L.), blueberry (Vaccinium corymbosum L.) and kiwiberry (Actinidia arguta (Siebold & Zucc.) Planch. ex Miq.) belong to the non-traditional and potentially attractive small fruits for consumers. The aim of this work was to examine adventitious regeneration ability of cultivars ‘Loch Ness’ (R. fruticosus), ‘Weiki’ (A. arguta), ‘Brigitta Blue’ and ‘Toro’ (V. corymbosum). Three types of explants (whole leaves /leaf segments in case of A. arguta/, petioles, and internodal segments) and various plant growth regulators were tested for their ability to induce adventitious shoots. Shoot multiplication was also evaluated. All cultivars tested were able to regenerate shoots from petioles and internodal segments, but leaves were only suitable for blueberry cultivars. Genotype-dependent differences in shoot induction and regeneration were found in blueberry. The highest regeneration rate (4.3 shoots/explant in mean) was achieved in blueberry cultivar ‘Brigitta Blue’ from whole leaves cultivated on WPM medium with 4 mg l−1 zeatin. Shoot multiplication was successful in all cultivars. Genetic stability of the V. corymbosum ‘Brigitta Blue’ regenerants was confirmed and no polymorphism was detected. Therefore, presented protocol can be used for further applications such as genetic transformation or plant germplasm conservation techniques.
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Abbreviations
- 2-iP:
-
2-Isopentenyladenine
- AFLP:
-
Amplified fragment length polymorphism
- BAP:
-
6-Benzylaminopurine
- CTAB:
-
Cetyltrimethylammonium bromide
- GA3 :
-
Gibberellic acid
- IAA:
-
Indole-3-acetic acid
- IBA:
-
Indole-3-butyric acid
- ISSR:
-
Inter Simple Sequence Repeat
- MS:
-
Murashige and Skoog medium
- NAA:
-
1-Naphthaleneacetic acid
- PGRs:
-
Plant growth regulators
- PVP:
-
Polyvinylpyrrolidone
- RAPD:
-
Random Amplified Polymorphic DNA
- TBE:
-
Tris-borate-acetate
- TDZ:
-
Thidiazuron
- WPM:
-
McCown Woody Plant medium
- ZEA:
-
Zeatin
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Acknowledgements
Authors would like to thank prof. Jana Žiarovská for the technical assistance with ISSR analysis.
Funding
This publication was supported by the Operational program Integrated Infrastructure within the project: Demand-driven research for the sustainable and inovative food, Drive4SIFood 313011V336, cofinanced by the European Regional Development Fund.
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JH wrote and edited the manuscript. JG performed the statistical analysis. JK contributed to the results. AG designed the experiment. JH and AG performed the final review of the manuscript.
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Hunková, J., Kleman, J., Gažo, J. et al. Adventitious regeneration of blackberry, blueberry, and kiwiberry and assessment of genetic stability by ISSR markers. Biologia 78, 349–359 (2023). https://doi.org/10.1007/s11756-022-01211-7
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DOI: https://doi.org/10.1007/s11756-022-01211-7