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Effect of electrohydrodynamic and ultrasonic pretreatments on the extraction of bioactive compounds from Melissa officinalis

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Abstract

The present study adopted electrohydrodynamic (EHD) and ultrasonic (US) pretreatments to enhance the extraction of phenolic compounds from Melissa officinalis. The effects of pretreatment time (EHD: 0–20 min and US: 0–60 min) and diffusion conditions (including ethanol/water ratios of 0.4, 0.8 and 1.2 (w/w), time of 30, 45, 60 min, and the temperature of 30, 50 and 70 °C) on the total phenolic content (TPC) and antioxidant activity of the extracts were investigated using Folin-Ciocalteu and DPPH assay, respectively. The results showed that EHD and US pretreatments could enhance the process of the extraction of phenolic compounds and also improve the extracts’ antioxidant activity. The highest extraction occurred at EHD time of 10 and 20 min, ethanol/water ratio of 1.2 and diffusion temperature of 70 °C. The Fourier transform infrared (FTIR) spectra also revealed that the structure of the extracted compounds was not altered as a result of EHD pretreatment. No changes, however, occurred in the functional groups of the extracts by increasing the EHD duration, diffusion temperature, and ethanol/water ratio.

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Acknowledgements

The authors would like to extend their gratitude to Dr. Seyed Ali Modarres hasani for English editing of the manuscript. The authors also express their sincere appreciation to Dr. Sima Maleki for her great assistance.

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ZHD: Investigation, Resources, Funding acquisition, Data curation, Writing. MN: Conceptualization, Methodology, Software, Formal analysis, Validation, Data curation, Writing- Review and Editing, Visualization, Supervision, Project administration, Funding acquisition. STD: Methodology, Validation, Writing- Review and Editing, Visualization, Supervision, Project administration.

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Correspondence to Moloud Nourani.

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Dastjerdi, Z.H., Nourani, M. & Dinani, S.T. Effect of electrohydrodynamic and ultrasonic pretreatments on the extraction of bioactive compounds from Melissa officinalis. Food Measure 16, 570–581 (2022). https://doi.org/10.1007/s11694-021-01183-3

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