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Plant regeneration of red raspberry (Rubus idaeus) cultivars ‘Joan J’ and ‘Polana’

  • Plant Tissue Culture
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Abstract

Plants were regenerated from in vitro leaf and petiole tissues of Rubus spp. L. (red raspberry) ‘Joan J’ and ‘Polana’ in woody plant medium (WPM), with 200 mg L−1 polyvinylpyrrolidone (PVP), and various combinations of 6-benzylaminopurine (BA) and/or thidiazuron (TDZ). The effects of explant type and explant age on plant regeneration were also investigated. Results showed that the regeneration rates were 70% for ‘Joan J’ and 82.2% for ‘Polana,’ when 7-d-old leaf tissues were inoculated on WPM with 2.5 μM BA + 1.0 μM TDZ and WPM with 2.5 μM BA + 0.1 μM TDZ, respectively. For ‘Joan J,’ in vitro rooting was readily achieved (100%) in one-half Murashige and Skoog (MS) medium supplemented with 10 μM 1-naphthaleneacetic acid (NAA). For ‘Polana,’ a rooting rate of 75% was achieved when cuttings were pulsed in one-half MS with 20 μM NAA for 7 d and then transferred to auxin-free one-half MS medium. An efficient ex vitro rooting frequency (> 80%) was obtained for both cultivars when cuttings were quickly dipped in 500 μM Indole-3-burytic acid (IBA) or NAA, and inserted to Sunshine Mix no. 1 (Fisons Western Corp., Vancouver, Canada) for a 4-wk rooting and acclimatization period. Regenerated plants were eventually grown in the greenhouse. This research will assist germplasm improvement using biotechnology techniques for raspberries and other Rubus species.

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Authors and Affiliations

  1. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA

    Changhyeon Kim & Wenhao Dai

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  1. Changhyeon Kim
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  2. Wenhao Dai
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Correspondence to Wenhao Dai.

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Editor: Marie-Anne Lelu-Walter

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Kim, C., Dai, W. Plant regeneration of red raspberry (Rubus idaeus) cultivars ‘Joan J’ and ‘Polana’. In Vitro Cell.Dev.Biol.-Plant 56, 390–397 (2020). https://doi.org/10.1007/s11627-019-10051-1

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  • DOI: https://doi.org/10.1007/s11627-019-10051-1

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