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Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots

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Abstract

Eucalyptus dunnii is an excellent alternative for afforestation and reforestation in frost-prone areas; however, it has limitations in the availability of propagation material. Given the great potential of tissue culture techniques for plant propagation and plant genetic transformation, the competence for in vitro organogenesis from E. dunnii cotyledons and hypocotyls was assessed. Several combinations of 6-benzylaminopurine (BAP) plus α-naphthalene acetic acid (NAA) or indole-3-acetic acid (IAA) were added (0, 0.1, 0.5, and 1 mg l−1) to a specifically developed basal medium. Moreover, the effect of the hypocotyl segments position (distal and proximal) on bud and callus regeneration was studied. Finally, microcuttings obtained from in vitro multiplication of the regenerated buds were treated with different dosages of indole-3-butyric acid (IBA) or NAA (powder based) and rooted under ex vitro conditions. Bud regeneration was observed only in hypocotyl segments, where dosages of 0.5 mg l−1 BAP plus 0.5 mg l−1 NAA or 0.5 mg l−1 IAA and distal segments resulted in higher bud regeneration rates. Histological analyses suggest that callus regeneration areas in hypocotyl explants recreate an appropriate niche for the development of pluripotent cells that give rise to buds and shoots. Either IBA or NAA treatments failed to significantly improved the ex vitro rooting of E. dunnii microcuttings, whereas >50% microcuttings rooted without application of growth regulators.

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Acknowledgments

The authors thank the Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo (ESALQ/USP) and the Instituto Nacional de Tecnología Agropecuaria (INTA) for the support.

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  1. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais, Universidade de São Paulo, Av. Pádua Dias, no 11, Caixa Postal 09, 13418-900, Piracicaba, São Paulo, Brazil

    Gustavo Pedro Javier Oberschelp, Antônio Natal Gonçalves & Marcílio de Almeida

  2. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Biológicas, PPG em Fisiologia e Bioquímica de Plantas, Universidade de São Paulo, Av. Pádua Dias, no 11, Caixa Postal 09, 13418-900, Piracicaba, São Paulo, Brazil

    Eveline Calderan Meneghetti, Érika Mendes Graner & Marcílio de Almeida

  3. Instituto Nacional de Tecnología Agropecuaria, EEA Concordia, Estación Yuquerí s/n s/n CC 54, Concordia, Entre Ríos, Argentina

    Gustavo Pedro Javier Oberschelp

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Oberschelp, G.P.J., Gonçalves, A.N., Meneghetti, E.C. et al. Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots. In Vitro Cell.Dev.Biol.-Plant 51, 626–636 (2015). https://doi.org/10.1007/s11627-015-9715-8

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