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In vitro plant regeneration from cotyledon and hypocotyl segments in two bell pepper cultivars

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Abstract

In vitro plant regeneration has been obtained from Capsicum annuum cvs. Pico and Piquillo. Shootbuds were induced from hypocotyl and cotyledon segments after 15–20 days of culture on MS basal medium supplemented with IAA and BAP or Zeatin. Shoot-buds grew into rosettes that rooted in MS plus NAA (0.1 mg/l) and IBA (0.05 mg/l) after 15 days. The small plantlets were successfully transferred to pots with a mixture of peat and perlite and maintained under greenhouse conditions. Elongation took place when the plantlets were growing in the greenhouse.

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Abbreviations

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

Kn:

Kinetin

MS:

Murashige and Skoog (1962)

NAA:

alpha-naphthalenacetic acid

Z:

Zeatin

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Authors and Affiliations

  1. Centro de Investigaciones Agrarias, Comunidad Autónoma de La Rioja, Apdo. 1056, Logroño, Spain

    Rosa Arroyo

  2. Departamento B.O.S. Lab. Fisiología Vegetal, Facultad de Biología, Universidad de Oviedo, 33005, Oviedo, Spain

    M. Angeles Revilla

Authors
  1. Rosa Arroyo
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  2. M. Angeles Revilla
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Communicated by R. N. Beachy

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Arroyo, R., Revilla, M.A. In vitro plant regeneration from cotyledon and hypocotyl segments in two bell pepper cultivars. Plant Cell Reports 10, 414–416 (1991). https://doi.org/10.1007/BF00232614

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  • DOI: https://doi.org/10.1007/BF00232614

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