Abstract
Purpose
Contaminated sediments are an important exposure pathway for the aquatic fauna in the Rhine River. We applied bioassays with the aim to characterize the ecotoxicological hazard potential of sediments of an oxbow lake of the Rhine River, especially to fish. Potential effects on fish and water flea were evaluated indirectly by applying in vitro and in vivo bioassays in the laboratory. Results were compared with those of the official German risk assessment of dredged sediments.
Materials and methods
Sediments taken from 13 sites along a 600-m transect line were tested for acute toxicity to water flea (Daphnia magna immobilization test), teratogenicity, and embryotoxicity (sediment contact test with Danio rerio), as well as for cytotoxicity (neutral red retention assay with RTL-W1 cells) and estrogenic effects (lyticase-assisted yeast estrogen screen (L-YES) assay). The tests were conducted using pore water, organic extracts, or native sediments. Spatial patterns of the measured effects were also assessed.
Results and discussion
Virtually all samples induced estrogenic, teratogenic, embryotoxic, and cytotoxic effects, but no acute toxicity on D. magna was observed. Cytotoxicity was in accordance with previous studies on the Rhine, Neckar, and Danube Rivers. Estrogenic effects were in the range of estradiol equivalent (EEQ) values detected in UK estuaries. Although sediment contact tests with D. rerio embryos showed virtually no mortality, sublethal effects were common. Some of the effects increased with increasing distance to the main channel.
Conclusions
The test with D. magna is, along with bacteria and algae toxicity assays, an important part of the German standard risk assessment for sediments. However, it failed to identify the ecological hazard of our sediment samples to fish. Our results indicate that adverse effects on fish are possible and suggest the need for revising risk assessment procedures in order to address the risk for this important organism group in aquatic ecosystems.
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References
Admiraal W, Van der Velde G, Smit H, Cazemier WG (1993) The rivers Rhine and Meuse in the Netherlands: present state and signs of ecological recovery. Hydrobiologia 265:97–128
Ahlf W (1995) Biotests an Sedimenten. In: Steinberg C, Bernhardt H, Klappner H (eds) Handbuch Angewandte Limnologie Teil Aquatische Ökotoxikologie. ecomed Verlag, Landsberg, Germany, pp 1–43
Ahlf W, Hollert H, Neumann-Hensel H, Ricking M (2002) A guidance for the assessment and evaluation of sediment quality: a German approach based on ecotoxicological and chemical measurements. J Soils Sediments 2:37–42
Allner B (2003) Freilanduntersuchungen zur Geschlechterverteilung einheimischer Fischpopulationen. Gobio GmbH, Frankfurt, Germany
Asselman NEM, Middelkoop H (1995) Floodplain sedimentation: quantities, patterns and processes. Earth Surface Process Landforms 20:481–499
Babich H, Borenfreund E (1992) Neutral red assay for toxicology in vitro. In: Watson RR (ed) In vitro methods of toxicology. CRC - Press, Boca Raton, USA, pp 238–251
Behrens A, Schirmer K, Bols NC, Segner H (2001) Polycyclic aromatic hydrocarbons as inducers of cytochrome P4501A enzyme activity in the rainbow trout liver cell line, RTL-W1, and in primary cultures of rainbow trout hepatocytes. Environ Toxicol Chem 20:632–643
BfG (2000) Handlungsanweisung für den Umgang mit Baggergut im Binnenland (HABAB-WSV). Koblenz, Germany, p 35
BfG (2004) Untersuchung und Beurteilung von Sedimentablagerungen im Lampertheimer Altrhein. Altrhein-km 0,0 bis 2,1; Altrhein-Mündung bei Rhein-km 440,3. Koblenz, Germany
BfG (2005) BfG 1474: Ergebnisse aus dem begleitenden Untersuchungsprogramm für die Umlagerung von Baggergut in die fließende Welle unterhalb der Staustufe Iffezheim/Rhein. Koblenz, Germany
BfG (2007) Standardarbeitsanweisung. SOP 1–04. BfG, Koblenz, Germany, p 5
Brack W, Klamer HJC, Lopez de Alda MJ, Barceló D (2007) Effect-directed analysis of key toxicants in European River Basins. A review. Environ Sci Pollut Res 14:30–38
Braunbeck T, Böttcher M, Hollert H, Kosmehl T, Lammer E, Leist E, Rudolf M, Seitz N (2005) Towards an alternative for the acute fish LC50 test in chemical assessment: the fish embryo toxicity test goes multi-species—an update. ALTEX 22:87–102
Braunbeck T, Brauns A, Keiter S, Hollert H, Schwartz P (2009) Fischpopulationen unter Stress–das Beispiel des Unteren Neckars. Umweltwissenschaften und Schadstoff-Forschung 21:197–211
Brian JV, Harris CA, Scholze M, Backhaus T, Booy P, Lamoree M, Pojana G, Jonkers N, Runnalls T, Bonfà A, Marcomini A, Sumpter JP (2005) Accurate prediction of the response of freshwater fish to a mixture of estrogenic chemicals. Environ Health Perspect 113:721
Brühne M, Scharbert A (2003) Die Erschließung des Bienener Altrheins für die Rheinfischfauna http://www.nz-kleve.de/fileadmin/dokumente/fachartikel/Br%C3%BChne_Scharbert_Dornicker-Schleuse_gesch%C3%BCtzt.pdf Accessed 26 May 2015:1–15
Burton GA (1991) Assessing the toxicity of freshwater sediments. Environ Toxicol Chem 10:1585–1627
Charles GD, Gennings C, Zacharewski TR, Gollapudi BB, Carney EW (2002) An approach for assessing estrogen receptor-mediated interactions in mixtures of three chemicals: a pilot study. Toxicol Sci 68:349
de Zwart D, Slooff W (1993) The pT value as an environmental toxicity indicator. Sci Total Environ Suppl 1993:1203–1210
DIN (2001) Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung: Suborganismische Testverfahren (Gruppe T) Teil 6: Giftigkeit gegenüber Fischen: Bestimmung der nicht akut giftigen Wirkung von Abwasser auf die Entwicklung von Fischeiern über Verdünnungsstufen (T 6). DIN 38415–6. Beuth Verlag, Berlin, Germany
Duong CN, Schlenk D, Chang NI, Kim SD (2009) The effect of particle size on the bioavailability of estrogenic chemicals from sediments. Chemosphere 76:395–401
Feiler U, Höss S, Ahlf W, Gilberg D, Hammers-Wirtz M, Hollert H, Meller M, Neumann-Hensel H, Ottermanns R, Seiler TB, Spira D, Heiniger P (2013) Sediment contact tests as a tool for the assessment of sediment quality in German waters. Environ Toxicol Chem 32:144–155
Goldsmith P, Harris WA (2003) The zebrafish as a tool for understanding the biology of visual disorders. Seminars Cell Develop Biol 14:11–18
Grund S, Higley E, Schönenberger R, Suter MJ, Giesy JP, Braunbeck T, Hecker M, Hollert H (2011) The endocrine disrupting potential of sediments from the Upper Danube River (Germany) as revealed by in vitro bioassays and chemical analysis. Environ Sci Pollut Res 18:446–460
Hallare AV, Seiler T-B, Hollert H (2011) The versatile, changing, and advancing roles of fish in sediment toxicity assessment—a review. J Soils Sediments 11:141–173
Harkey GA, Landrum PF, Klaine SJ (1994) Comparison of whole‐sediment, elutriate and pore‐water exposures for use in assessing sediment‐associated organic contaminants in bioassays. Environ Toxicol Chem 13:1315–1329
Hayes TB, Case P, Chui S, Chung D, Haeffele C, Haston K, Lee M, Mai PV, Marjuoa Y, Parker J, Tsui M (2006) Pesticide mixtures, endocrine disruption, and amphibian declines: are we underestimating the impact? Environ Health Perspect 114:40–50
Hecker M, Hollert H (2009) Effect-directed analysis (EDA) in aquatic ecotoxicology: state of the art and future challenges. Environ Sci Pollut Res 16:607–613
Heger S, Blum K, Agler MT, Maletz S, Schäffer A, Seiler T-B, Angenent LT, Hollert H (2012) Biotests for hazard assessment of biofuel fermentation. Energy Environ Sci 5:9778–9788
Heimann W, Sylvester M, Seiler TB, Hollert H, Schulz R (2011) Sediment toxicity in a connected oxbow lake of the Upper Rhine (Germany): EROD induction in fish cells. J Soils Sediments 11:1279–1291
Heise S, Förstner U (2006) Risks from historical contaminated sediments in the Rhine basin, the interactions between sediments and water. Springer, Heidelberg, pp 261–272
Hollert H, Durr M, Erdinger L, Braunbeck T (2000) Cytotoxicity of settling particulate matter and sediments of the Neckar River (Germany) during a winter flood. Environ Toxicol Chem 19:528–534
Hollert H, Keiter S, König N, Rudolf M, Ulrich M, Braunbeck T (2003) A new sediment contact assay to assess particle-bound pollutants using zebrafish (Danio rerio) embryos. J Soils Sediments 3:197–207
Höss S, Ahlf W, Fahnenstich C, Gilberg D, Hollert H, Melbye K, Meller M, Hammers-Wirtz M, Heininger P, Neumann-Hensel H, Ottermanns R, Ratte HT, Seiler TB, Spira D, Weber J, Feiler U (2010) Variability of sediment-contact tests in freshwater sediments with low-level anthropogenic contamination—determination of toxicity thresholds. Environ Pollut 158:2999–3010
Huberman E, Sachs L, Yang SK, Gelboin V (1976) Identification of mutagenic metabolites of benzo (a) pyrene in mammalian cells. Proc Natl Acad Sci USA 73:607
Hünemörder KF (2004) Die Frühgeschichte der globalen Umweltkrise und die Formierung der deutschen Umweltpolitik (1959–1973) vol 53. Historische Mitteilungen - Beihefte. Franz Steiner Verlag, Stuttgart, Germany
IKSR (2002) Kontamination von Rheinfischen 2000. Berichte der Internationalen Kommision zum Schutz des Rheins (IKSR) 124:76
IKSR (2003) Rhein Bestandsaufnahme der Emissionen prioritärer Stoffe 2000. Berichte der Internationalen Kommision zum Schutz des Rheins (IKSR) 134:77
IKSR (2007) Qualitätskomponente Fische – Monitoring Rheinfischfauna. Berichte der Internationalen Kommision zum Schutz des Rheins (IKSR) 173:89
IKSR (2009a) Geschichte - Bilanz des Aktionsprogramms (1986–2000).1
IKSR (2009b) Sedimentmanagementplan Rhein. Berichte der Internationalen Kommision zum Schutz des Rheins (IKSR) 175:101
Janssen PAH, Lambert JGD, Vethaak A, Goos HJT (1997) Environmental pollution caused elevated concentrations of oestradiol and vitellogenin in the female flounder, Platichthys flesus (L). Aquatic Toxicol 39:195–214
Jezierska B, Lugowska K, Witeska M (2009) The effects of heavy metals on embryonic development of fish (a review). Fish Physiol Biochem 35:625–640
Kammann U, Vobach M, Wosniok W (2006) Toxic effects of brominated indoles and phenols on zebrafish embryos. Arch Environ Contamin Toxicol 51:97–102
Kay AL, Jones RG, Reynard NS (2006) RCM rainfall for UK flood frequency estimation. II. Climate change results. J Hydrol 318:163–172
Keiter S, Rastall A, Kosmehl T, Wurm K, Erdinger L, Braunbeck T, Hollert H (2006) Ecotoxicological assessment of sediment, suspended matter and water samples in the upper Danube River—a pilot study in search for the causes for the decline of fish catches. Environ Sci Pollut Res 13:308–319
Kidd KA, Blanchfield PJ, Mills KH, Palace VP, Evans RE, Lazorchak JM, Flick RW (2007) Collapse of a fish population after exposure to a synthetic estrogen. Proc Natl Acad Sci U S A 104:8897–8901
Klee N, Gustavsson L, Kosmehl T, Engwall M, Erdinger L, Braunbeck T, Hollert H (2004) Changes in toxicity and genotoxicity of industrial sewage sludge samples containing nitro- and amino-aromatic compounds following treatment in bioreactors with different oxygen regimes. Environ Sci Pollut Res 11:313–320
König N (2002) Der Zebrabärbling (Danio rerio) in Fischeitest und Comet-Assay - Embryotoxikologische Untersuchung von Rheinsedimenten, Diploma thesis. Ruprecht-Karls-Universität Heidelberg, Germany, p 64
Kosmehl T, Krebs F, Manz W, Braunbeck T, Hollert H (2007) Differentiation between bioavailable and total hazard potential of sediment-induced DNA fragmentation as measured by the comet assay with zebrafish embryos. J Soils Sediments 7:377–387
Krebs F (1999) The pT-Method as a Hazard Assessment Scheme for sediments and dredged material. In: Blaise C, Férard J-F (eds) Small-scale freshwater toxicity investigations, vol 2. Springer Netherlands, Dordrecht, Germany, pp 115–137
Kukkonen J, Landrum PF (1996) Distribution of organic carbon and organic xenobiotics among different particle-size fractions in sediments. Chemosphere 32:1063–1076
Länge R, Hutchinson TH, Croudace CP, Siegmund F, Schweinfurth H, Hampe P, Panter GH, Sumpter JP (2001) Effects of the synthetic estrogen 17 alpha-ethinylestradiol on the life-cycle of the fathead minnow (Pimephales promelas). Environ Toxicol Chem 20:1216–1227
Legler J, Dennekamp M, Vethaak AD, Brouwer A, Koeman JH, van der Burg B, Murk AJ (2002) Detection of estrogenic activity in sediment-associated compounds using in vitro reporter gene assays. Sci Total Environ 293:69–83
Leppänen M (1995) The role of feeding behaviour in bioaccumulation of organic chemicals in benthic organisms. Annales Zoologici Fennici 32:247–255
Lopez de Alda MJ, Barceló D (2001) Use of solid-phase extraction in various of its modalities for sample preparation in the determination of estrogens and progestogens in sediment and water. J Chromatography A 938:145–153
LUA LR-P (2006) Fischmonitoring Rheinland · Pfalz. Untersuchungsergebnisse aus den Jahren 1999, 2000, 2001 und 2003. Landesuntersuchungsamt Rheinland-Pfalz, Abteilung Lebensmittelchemie, Koblenz, Germany
Lund BO, Bergman A, Brandt I (1988) Metabolic activation and toxicity of a DDT-metabolite, 3-methylsulphonyl-DDE, in the adrenal zona fasciculata in mice. Chem Biol Interact 65:25–40
LUWG, Otto A, Weibel U (1999) Entwicklung der Rhein-Auengewässer - Teil 2 - Entwicklungsplan. Mainz, Germany
Metcalfe CD, Metcalfe TL, Kirparissis Y, Koenig BG, Khan C, Hughes RJ, Croley TR, March RE, Potter T (2001) Estrogenic potency of chemicals detected in sewage treatment plant effluents as determined by in vivo assays with Japanese medaka (Oryzias latipes). Environ Toxicol Chem 20:297–308
Molls F (1999) New insights into the migration and habitat use by bream and white bream in the floodplain of the River Rhine. J Fish Biol 55
MUFV R (2006) VSG 6816–403 „Karlskopf und Leimersheimer Altrhein“. Natura 2000 - Steckbriefe. Ministerium für Umwelt, Forsten und Verbraucherschutz Rheinland Pfalz, Germany
Nagel R (2002) DarT: the embryo test with the Zebrafish Danio rerio a general model in ecotoxicology and toxicology. ALTEX 19:38–48
OECD (2004) OECD guideline for testing of chemicals. Daphnia sp. acute immobilisation test (202). OECD Publishing, Paris. doi: http://dx.doi.org/10.1787/9789264069947-en
Pawlowski S, Ternes T, Bonerz M, Kluczka T, van der Burg B, Nau H, Erdinger L, Braunbeck T (2003) Combined in situ and in vitro assessment of the estrogenic activity of sewage and surface water samples. Toxicol Sci 75:57–65
Rocha PS, Luvizotto GL, Kosmehl T, Boettcher M, Storch V, Braunbeck T, Hollert H (2009) Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies. Ecotox Environ Safe 72:1842–1848
Routledge EJ, Sumpter JP (1996) Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen. Environ Toxicol Chem 15:241–248
Schmitt C, Oetken M, Dittberner O, Wagner M, Oehlmann J (2008) Endocrine modulation and toxic effects of two commonly used UV screens on the aquatic invertebrates Potamopyrgus antipodarum and Lumbriculus variegatus. Environ Pollut 152:322–329
Schultis T, Metzger JW (2004) Determination of estrogenic activity by LYES-assay (yeast estrogen screen-assay assisted by enzymatic digestion with lyticase). Chemosphere 57:1649–1655
Schulze T, Ulrich M, Maier D, Maier M, Terytze K, Braunbeck T, Hollert H (2015) Evaluation of the hazard potentials of river suspended particulate matter and floodplain soils in the Rhine basin using chemical analysis and in vitro bioassays. Environ Sci Pollut Res 22:14606–14620
Seiler T-B, Rastall AC, Leist E, Erdinger L, Braunbeck T, Hollert H (2006) Membrane dialysis extraction (MDE): a novel approach for extracting toxicologically relevant hydrophobic organic compounds from soils and sediments for assessment in biotests. J Soils Sediments 6:20–29
Seiler T-B, Schulze T, Hollert H (2008) The risk of altering soil and sediment samples upon extract preparation for analytical and bio-analytical investigations—a review. Analytical Bioanalytical Chem 390:1975–1985
Solomon KR, Sibley P (2002) New concepts in ecological risk assessment: where do we go from here? Marine Pollut Bull 44:279–285
Solomon S, Qin D, Manning M, Chen Z, Marrquis M, Averyt KB, Tignor M, Miller HL (2007) Working group I contribution to the fourth assessment report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge, UK; New York, NY, USA
Strecker R, Seiler TB, Hollert H, Braunbeck T (2011) Oxygen requirements of zebrafish (Danio rerio) embryos in embryo toxicity tests with environmental samples. Comparative Biochem Physiol Part C: Toxicol Pharmacol 153:318–327
Thomas KV, Balaam J, Hurst M, Nedyalkova Z, Mekenyan O (2004) Potency and characterization of estrogen-receptor agonists in United Kingdom estuarine sediments. Environ Toxicol Chem 23:471–479
Tittizer T, Krebs F (1996) Ökosystemforschung: Der Rhein und seine Auen - eine Bilanz. Springer, Berlin, Heidelberg, Germany
UBA (2007) Ökologische Neuorientierung der Bundeswasserstraßenbewirtschaftung (UBA Texte 40/07). Umweltbundesamt, Dessau, Germany
Ulrich M (2002) Gefährdung von Trinkwasser durch partikulär gebundene Schadstoffe in den Rheinauen: Vergleich nativer und extrahierter Proben in zwei in vitro Biotests Diploma thesis. Ruprecht-Karls-Universität Heidelberg, Germany, p 73
USEPA (1996) Method 3660B—sulfur cleanup. US EPA Methods, US Government Printing Office, Washington, pp 1–6
Van den Brink FWB, Van Katwijk MM, Van der Velde G (1994) Impact of hydrology on phyto- and zooplankton community composition in floodplain lakes along the Lower Rhine and Meuse. J Plankton Res 16:351
Vincze K, Graf K, Scheil V, Köhler H-R, Triebskorn R (2014) Embryotoxic and proteotoxic effects of water and sediment from the Neckar River (Southern Germany) to zebrafish (Danio rerio) embryos. Environ Sci Eur 26:1–13
Wagner M, Oehlmann J (2009) Endocrine disruptors in bottled mineral water: total estrogenic burden and migration from plastic bottles. Environ Sci Pollut Res 16:278–286
Wernersson A-S, Carere M, Maggi C, Tusil P, Soldan P, James A, Sanchez W, Dulio V, Broeg K, Reifferscheid G, Buchinger S, Maas H, Van Der Grinten E, O’Toole S, Ausili A, Manfra L, Marziali L, Polesello S, Lacchetti I, Mancini L, Lilja K, Linderoth M, Lundeberg T, Fjällborg B, Porsbring T, Larsson DJK, Bengtsson-Palme J, Förlin L, Kienle C, Kunz P, Vermeirssen E, Werner I, Robinson CD, Lyons B, Katsiadaki I, Whalley C, den Haan K, Messiaen M, Clayton H, Lettieri T, Negrão Carvalho R, Gawlik BM, Hollert H, Di Paolo C, Brack W, Kammann U, Kase R (2015) The European technical report on aquatic effect-based monitoring tools under the water framework directive. Environ Sci Eur 27:1–11
Wölz J, Cofalla C, Hudjetz S, Roger S, Brinkmann M, Schmidt B, Schäffer A, Kammann U, Lennartz G, Hecker M, Schüttrumpf H, Hollert H (2009) In search for the ecological and toxicological relevance of sediment re-mobilisation and transport during flood events. J Soils Sediments 9:1–5
Acknowledgments
The present study was embedded into a project by the University Koblenz-Landau investigating the anthropogenic impact on fish populations in the Rhine River. We received funding by the Structural and Approval Directorate South (SGD Süd) and Ministry for Environment, Forestry and Consumer Protection Rhineland-Palatinate, Germany. The authors would like to thank Dr. N.C. Bols and Dr. L. Lee (University of Waterloo, Canada) for providing RTL-W1 cells and Dr. J. Sumpter for providing yeast cells. Furthermore, we thank Dr. F. Sylvester for helpful comments on the manuscript.
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Schulze-Sylvester, M., Heimann, W., Maletz, S. et al. Are sediments a risk? An ecotoxicological assessment of sediments from a quarry pond of the Upper Rhine River. J Soils Sediments 16, 1069–1080 (2016). https://doi.org/10.1007/s11368-015-1309-x
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DOI: https://doi.org/10.1007/s11368-015-1309-x