Abstract
It is well known that polyploidy is a major force in plant evolution and diversification. Evaluating the transmission of heterozygosity by 2n gametes with different formation mechanisms is of crucial importance. In the present study, 120 triploid hybrids [originating from first-division restitution (FDR), second-division restitution (SDR), and postmeiotic restitution (PMR) 2n eggs of the same female parent] and 30 pairs of polymorphic simple sequence repeat (SSR) markers (distributed across all 19 chromosomes of Populus) were used to evaluate the maternal heterozygosity transmitted by each type of 2n eggs. FDR, SDR, and PMR 2n eggs transmitted different parental heterozygosities (0.7480, 0.3958, and 0.3590, respectively) in Populus. In addition, compared with the small coefficient of variation (CV = 13.50 %) of heterozygosity transmitted by the 19 chromosomes of FDR 2n eggs, there was greater variation in heterozygosity transmitted by SDR and PMR 2n eggs (CV = 51.92 % and CV = 63.14 %, respectively). This may have resulted from the locations of the applied SSR markers, in addition to the different formation mechanisms of 2n gametes. Variations in locus-centromere distance resulted in variations in recombination rates, which influence the degree of heterozygosis at the detected locus. In conclusion, our data provides a useful reference point for the suitable applications of 2n gametes in future studies, promoting the further research of polyploidy in plants.
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References
Barone A, Gebhardt C, Frusciante L (1995) Heterozygosity in 2n gametes of potato evaluated by RFLP markers. Theor Appl Genet 91:98–104
Bastiaanssen HJ, Van Den Berg PM, Lindhout P, Jacobsen E, Ramanna M (1998) Postmeiotic restitution in 2n-egg formation of diploid potato. Heredity 81:20–27
Burnham CR (1962) Discussions in cytogenetics. Burgess Publishing, Minneapolis
Carputo D, Frusciante L, Peloquin SJ (2003) The role of 2n gametes and endosperm balance number in the origin and evolution of polyploids in the tuber-bearing Solanums. Genetics 163:287–294
Copenhaver GP, Nickel K, Kuromori T, Benito MI, Kaul S, Lin X, Bevan M, Murphy G, Harris B, Parnell LD, McCombie WR, Martienssen RA, Marra M, Preuss D (1999) Genetic definition and sequence analysis of Arabidopsis centromeres. Science 286:2468–2474
Dong CB, Suo YJ, Kang XY (2014) Assessment of the genetic composition of triploid hybrid Populus using SSR markers with low recombination frequencies. Can J For Res. doi:10.1139/cjfr-2013-0360
Evans E, Alani E (2000) Roles for mismatch repair factors in regulating genetic recombination. Mol Cell Biol 20:7839–7844
Grant V (1971) Plant speciation. Columbia University Press, New York
Harlan JR (1975) On Ö. Winge and a prayer: the origins of polyploidy. Bot Rev 41:361–390
Hermsen J (1984) Mechanisms and genetic implications of 2n gamete formation. Iowa State J Res 58:421–434
Hulce D, Li X, Snyder-Leiby T, Liu CJ (2011) GeneMarker® genotyping software: tools to Increase the statistical power of DNA fragment analysis. J Biomol Tech 22:S35
Jones GH (1984) The control of chiasma distribution. Symp Soc Exp Biol 38:293–320
Lim KB, Ramanna MS, de Jong JH, Jacobsen E, van Tuyl JM (2001) Indeterminate meiotic restitution (IMR): a novel type of meiotic nuclear restitution mechanism detected in interspecific lily hybrids by GISH. Theor Appl Genet 103:219–230
Lindner K, Seeb J, Habicht C, Knudsen K, Kretschmer E, Reedy D, Spruell P, Allendorf F (2000) Gene-centromere mapping of 312 loci in pink salmon by half-tetrad analysis. Genome 43:538–549
Little TM (1945) Gene segregation in autotetraploids. Bot Rev 11:60–85
Little TM (1958) Gene segregation in autotetraploids. II. Bot Rev 24:318–339
Mok D, Peloquin S (1975) The inheritance of three mechanisms of diplandroid (2n pollen) formation in diploid potatoes. Heredity 35:295–302
Nachman MW (2002) Variation in recombination rate across the genome: evidence and implications. Curr Opin Genet Dev 12:657–663
Otto SP (2007) The evolutionary consequences of polyploidy. Cell 131:452–462
Peloquin S (1982) Meiotic mutants in potato breeding. Stadler Symp, pp 99–109
Peloquin SJ, Boiteux LS, Simon PW, Jansky SH (2008) A chromosome-specific estimate of transmission of heterozygosity by 2n gametes in potato. J Hered 99:177–181
Ramanna M (1979) A re-examination of the mechanisms of 2n gamete formation in potato and its implications for breeding. Euphytica 28:537–561
Sang T, Pan J, Zhang D, Ferguson D, Wang C, Pan K-Y, Hong D-Y (2004) Origins of polyploids: an example from peonies (Paeonia) and a model for angiosperms. Biol J Linn Soc 82:561–571
Schmid C, Deka N, Matera A (1989) In: Adolph KW (ed) Chromosomes: eukaryotic, prokaryotic, and viral. CRC Press, Boca Raton
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234
Surtees JA, Argueso JL, Alani E (2004) Mismatch repair proteins: key regulators of genetic recombination. Cytogenet Genome Res 107:146–159
Tang X, Luo Z (2002) Cytology of 2n pollen formation in nonastringent persimmon. Sci Agric Sin 35:585–588
Tuskan GA, Gunter LE, Yang ZK, Yin TM, Sewell MM, DiFazio SP (2004) Characterization of microsatellites revealed by genomic sequencing of Populus trichocarpa. Can J For Res 34:85–93
Wang J, Kang XY, Li DL, Chen HW, Zhang PD (2010) Induction of diploid eggs with colchicine during embryo sac development in Populus. Silvae Genet 59:40–48
Yan H, Jin W, Nagaki K, Tian S, Ouyang S, Buell CR, Talbert PB, Henikoff S, Jiang J (2005) Transcription and histone modifications in the recombination-free region spanning a rice centromere. Plant Cell 17:3227–3238
Yeh F, Yang R, Boyle T (1999) POPGENE ver. 1.32. Microsoft Windows-based freeware for population genetic analysis. Quick user guide. Center Int. For Res, Univ of Alberta, Edmonton
Younis A, Hwang YJ, Lim KB (2014) Exploitation of induced 2n-gametes for plant breeding. Plant Cell Rep 33:215–223
Acknowledgments
This work was financially supported by the Special Fund for Forest Scientific Research in the Public Welfare (201404113), the National Natural Science Foundation of China (31470667), and the 111 Project (B13007). We would like to acknowledge members of our labs for advice and discussions. We thank the Forestry Research Institute of Tongliao City, the Inner Mongolia Autonomous Region, People’s Republic of China, for collecting the plant material and for additional help.
Data archiving statement
SSR markers used in this study are from the International Populus Genome Consortium (IPGC, http://www.ornl.gov/sci/ipgc/ssr_resource.htm). Information of all the applied SSR markers are available as supplemental text files that can be downloaded with the manuscript.
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Communicated by A. Brunner
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Dong, CB., Suo, YJ., Wang, J. et al. Analysis of transmission of heterozygosity by 2n gametes in Populus (Salicaceae). Tree Genetics & Genomes 11, 799 (2015). https://doi.org/10.1007/s11295-014-0799-9
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DOI: https://doi.org/10.1007/s11295-014-0799-9