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Improved chromosome doubling of parthenogenetic haploid plants of cucumber (Cucumis sativus L.) using colchicine, trifluralin, and oryzalin

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Abstract

An effective chromosome doubling protocol was established in essential garden crop of cucumber (Cucumis sativus L.) Cv. Hi Power. The different concentrations of colchicine (0, 250, 500, 750, and 1500 mg/L), oryzalin (0, 5, 15, 25, 50, 75, and 150 mg/L) and trifluralin (0, 5, 15, 25, 50, 75, and 150 mg/L) were applied on parthenogenesis-induced haploid nodal and shoot tip explants of cucumber for 18 and 38 h in three independent factorial experiments. Increasing concentrations of applied antimitotic agents led to the significant reduction in the survival rate of both shoot tip and nodal explants, especially in longer exposure duration. Three ploidy levels including haploid, mixoploid, and doubled haploid were regenerated form both explant types treated with colchicine, oryzalin, and trifluralin. Flow cytometry analysis proved successful chromosome doubling of haploid plants. Based on the results obtained, the highest number of regenerated doubled haploid plants (92.31%) and fruit set (86.21%) were related to immersion of nodal explants in 50 mg/L oryzalin for 18 h. The highest doubled haploid regeneration for colchicine and trifluralin antimitotic agents were 58.33 and 83.33%, respectively. The leaf size of doubled haploid plants was larger than their correspond haploids. The optimized chromosome doubling protocol would be applicable for doubled haploid production in garden crops of Cucurbitaceae family, which is recalcitrant to the spontaneous doubling, and also for in vitro polyploidy induction studies.

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Acknowledgements

This research was supported by grants from Agricultural Biotechnology Research Institute of Iran (ABRII) Project No. 12-05-05-9451-94001.

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Authors and Affiliations

  1. Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

    Hamed Ebrahimzadeh, Hassan Soltanloo & Seiede Sanaz Ramezanpour

  2. Department of Tissue and Cell Culture, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, 3135933151, Iran

    Hamed Ebrahimzadeh & Mehran E. Shariatpanahi

  3. Nuclear Agriculture Research School- NSTRI, Karaj, Iran

    Ali Eskandari

Authors
  1. Hamed Ebrahimzadeh
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  2. Hassan Soltanloo
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  3. Mehran E. Shariatpanahi
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  4. Ali Eskandari
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  5. Seiede Sanaz Ramezanpour
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Contributions

HE contributed in the practical process of parthenogenesis induction, regeneration of plants, chromosome doubling, cytological and flow cytometer analysis. HS and MES (corresponding authors) contributed in the supervising of the whole process. AE contributed in the pollen irradiation experiments. SSR contributed in data analysis.

Corresponding authors

Correspondence to Hassan Soltanloo or Mehran E. Shariatpanahi.

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The authors declare that they have no conflict of interests.

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Communicated by Maria Antonietta Germanà.

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Ebrahimzadeh, H., Soltanloo, H., Shariatpanahi, M.E. et al. Improved chromosome doubling of parthenogenetic haploid plants of cucumber (Cucumis sativus L.) using colchicine, trifluralin, and oryzalin. Plant Cell Tiss Organ Cult 135, 407–417 (2018). https://doi.org/10.1007/s11240-018-1473-y

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  • DOI: https://doi.org/10.1007/s11240-018-1473-y

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