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In vitro induction of tetraploids in crape myrtle (Lagerstroemia indica L.)

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Abstract

A protocol for in vitro induction of crape myrtle tetraploids using nodes from in vitro-grown shoots (2n = 48) was established. Nodal buds were excised from in vitro-grown shoots, maintained on proliferation medium containing Murashige and Skoog medium supplemented with 4.44 μM 6-benzyladenine , 0.54 μM α-naphthaleneacetic acid, and treated with a range of concentrations of colchicine under three different conditions. Nodal bud explants treated in liquid proliferation medium supplemented with either 15 or 20 mM colchicine for 24 h turned necrotic and died; whereas, those cultured on solid proliferation medium supplemented with either 125 or 250 μM colchicine for 30 days survived, but no tetraploid plants were obtained. However, when explants were cultured in liquid proliferation medium containing 250, 500 or 750 μM colchicine for 10 days, tetraploid plants (2n = 96) were obtained. Incubation of explants in medium containing 750 μM colchicine promoted the highest frequency of survival (40%) of explants and of recovered tetraploids (60%). Morphological and anatomical characteristics of leaves, including leaf index, stomata size and number, stomata index (length/width), and number of chloroplasts in guard cells correlated with ploidy of crape myrtle plants. The number of chloroplasts in guard cells of stomata was a stable and reliable marker in discriminating plants of different ploidy levels. Chromosome counts and flow cytometry confirmed these findings.

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Abbreviations

BA:

6-Benzyladenine

NAA:

α-Naphthaleneacetic acid

MS:

Murashige and Skoog (1962) medium

DMSO:

Dimethyl sulfoxide

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Acknowledgments

This research was financially supported by The Science Foundation of College of Jinling Institute of Technology, China (JIT-RCYJ-2007001). We greatly appreciate Dr. Aliya Momotaz, Plant Geneticists, PFVRC, Wisconsin and Dr. Jiehua Wang for critically reviewing the manuscript and their useful comments.

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Authors and Affiliations

  1. College of Agriculture and Bio-Engineering, Tianjin University, 300072, Tianjin, Pepole’s Republic of China

    QinYing Zhang, Li Liu & FuChang Guo

  2. Jinling Institute of Technology, 210038, Nanjing, Pepole’s Republic of China

    FengXia Luo

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  1. QinYing Zhang
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  2. FengXia Luo
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  3. Li Liu
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  4. FuChang Guo
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Correspondence to QinYing Zhang.

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Zhang, Q., Luo, F., Liu, L. et al. In vitro induction of tetraploids in crape myrtle (Lagerstroemia indica L.). Plant Cell Tiss Organ Cult 101, 41–47 (2010). https://doi.org/10.1007/s11240-009-9660-5

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  • DOI: https://doi.org/10.1007/s11240-009-9660-5

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