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Characterization of the R2R3-MYB Transcription Factor CsMYB113 Regulates Anthocyanin Biosynthesis in Tea Plants (Camellia sinensis)

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Abstract

Tea plant (Camellia sinensis) has very long history of cultivation and abundant germplasm resources in China. Purple bud is a characteristic variety, which has attracted the attention of breeding researchers because it accumulated a large number of anthocyanins naturally. In many species, R2R3-MYB transcription factors (TFs) were proved to be involved in the regulation of anthocyanin biosynthesis. Research on anthocyanin metabolism has been relatively clear in some species, but that needs to be further elucidated in tea plants. In this research, an R2R3-MYB transcription factor CsMYB113 related to the anthocyanin accumulation regulation was identified from tea plants. Spatial and temporal expression analysis revealed differential expression of CsMYB113 among different tissues and organs, with highest expression occurring in the roots. Subcellular localization assays showed that CsMYB113 localized in the nucleus. Ectopic expression of CsMYB113 increased pigmentation and anthocyanin contents by the upregulation of the expression levels of genes in anthocyanin biosynthesis pathway among different tissues of Arabidopsis. Moreover, transient overexpression of 35S::CsMYB113 in tea plant increased the anthocyanin contents in the leaves. Our results indicated that CsMYB113 plays important role in the anthocyanin biosynthesis regulation in tea plants. It will also provide useful candidate gene for the modification of anthocyanin metabolism by genetic engineering in plants.

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Funding

This work was supported by the National Natural Science Foundation of China (32072622, 31600556) and the Fundamental Research Funds for the Central Universities, Huazhong Agricultural University (2662019PY045).

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Authors and Affiliations

  1. Key Laboratory of Horticulture Plant Biology, Ministry of Education, College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Liuyuan Shui, Wenyi Li, Meilin Yan, Hui Li & Fei Guo

  2. Ministry of Agriculture, College of Horticulture and Forestry Sciences, Key Laboratory of Urban Agriculture in Central China, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Liuyuan Shui, Wenyi Li, Meilin Yan, Hui Li & Fei Guo

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Contributions

LS and FG designed the experiments. LS, MY, WL, and HL performed the experiments. LS and FG wrote the manuscript. WL and FG revise the manuscript. All authors provided helpful discussions and approved its final version.

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Correspondence to Fei Guo.

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Key Message

• A R2R3-MYB transcription factor CsMYB113 clustered into S6 subgroup was identified in tea plant.

• CsMYB113 act as transcription factor and localize in the nucleus.

• Overexpression CsMYB113 in Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs.

• The anthocyanin contents were significantly increased in leaves transient transformed with CsMYB113 gene.

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Shui, L., Li, W., Yan, M. et al. Characterization of the R2R3-MYB Transcription Factor CsMYB113 Regulates Anthocyanin Biosynthesis in Tea Plants (Camellia sinensis). Plant Mol Biol Rep 41, 46–58 (2023). https://doi.org/10.1007/s11105-022-01348-4

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