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Colorimetric detection of glutathione by an anionic pyridylazo dye-based Cu2+ complex in the presence of a cationic polyelectrolyte

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Abstract

The colorimetric sensing system for glutathione (GSH) was constructed by the mixture of three components, an anionic dye 2-(5-bromo-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino)phenol (BrPAPS), a cationic polyelectrolyte poly(diallyldimethylammonium chloride) (PDADMAC), and Cu2+ in an aqueous solution. BrPAPS was complexed with Cu2+ in a 2:1 molar ratio via the electrostatic interaction with PDADMAC (BrPAPS2-Cu2+-PDADMAC). The addition of GSH to the complex caused a hypsochromic shift with a distinct colorimetric response from red to yellow since the regeneration of the original BrPAPS through the interaction between GSH and Cu2+ in a 1:1 stoichiometry. BrPAPS2-Cu2+-PDADMAC allowed the selective recognition of GSH over other amino acids and the detection suffer little interference from them. Hence, BrPAPS2-Cu2+-PDADMAC provides efficient colorimetric sensing of GSH with high selectivity and sensitivity in pure aqueous media.

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Authors and Affiliations

  1. Faculty of Science and Engineering, Toyo University, 2100, Kujirai, Kawagoe, Saitama, 350-8585, Japan

    Yasumasa Fukushima

  2. Graduate School of Science and Engineering, Toyo University, 2100, Kujirai, Kawagoe, Saitama, 350-8585, Japan

    Mahiro Shimizu & Yoshiaki Koizumi

  3. Research Institute of Industrial Technology, Toyo University, 2100, Kujirai, Kawagoe, Saitama, 350-8585, Japan

    Shunichi Aikawa

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  1. Mahiro Shimizu
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  2. Yoshiaki Koizumi
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  3. Shunichi Aikawa
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  4. Yasumasa Fukushima
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MS: and YK: performed absorption measurements. SA: prepared all figures. YF: wrote the main manuscript and supervised the study. All authors reviewed the manuscript.

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Correspondence to Yasumasa Fukushima.

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Shimizu, M., Koizumi, Y., Aikawa, S. et al. Colorimetric detection of glutathione by an anionic pyridylazo dye-based Cu2+ complex in the presence of a cationic polyelectrolyte. J Incl Phenom Macrocycl Chem 103, 123–129 (2023). https://doi.org/10.1007/s10847-023-01183-4

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