Abstract
Eugenia pohliana DC.(Myrtaceae) is used in folk medicine by communities in Brazil. However, there are no reports on its biological activity. This is the first study to identify the components of E. pohliana essential oil (EpEO) and evaluate their antinociceptive and anti-inflammatory activities in an in vivo model at doses of 25, 50, and 100 mg/kg. The essential oil (EO) was obtained by hydrodistillation, and the analysis was performed by gas chromatography coupled with mass spectrometry. Antinociceptive activity was evaluated by writhing tests, tail movement, and formalin (neurogenic and inflammatory pain); naloxone was used to determine the nociception mechanism. Anti-inflammatory activity was assessed by oedema and peritonitis tests. We found that (E)-β-caryophyllene (BCP) (15.56%), δ-cadinene (11.24%) and α-cadinol (10.89%) were the major components. In the writhing test, there was a decrease in writing by 42.95–70.70%, in the tail movement, an increase in latency time by 69.12–86.63%, and in the formalin test, there was a reduction in pain neurogenic by 29.54–61.74%, and inflammatory pain by 37.42–64.87%. The antinociceptive effect of EpEO occurs through the activation of opioid receptors. In addition, a reduction in inflammation by 74.93‒81.41% was observed in the paw edema test and inhibition of the influx of leukocytes by 51.86‒70.38% and neutrophils by 37.74‒54.72% in the peritonitis test. It was concluded that EpEO has antinociceptive effect by the opioid pathway, as shown by the inhibitory effect of naloxone, and anti-inflammatory actions, and that its use does not cause hemolytic damage or behavioral change.
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We declare for all purposes that the National Council for Scientific Research and Development (CNPq), the Coordination for the Improvement of Higher Education Personnel (Capes), and the Fundação de Amparo a Ciência e Tecnologia do Estado de Pernambuco (FACEPE) have made available scholarships of research and financial support to researchers, for the acquisition of inputs and materials.
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The authors express their gratitude to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for financial support and investigator research grants (DMAFN, THN, and MTSC). We are also grateful to the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES; Finance Code 001) and the Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE) for financial support.
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ALD, JBG, WKC, AMO performed the methodologies. WKC, AMO analyzed and interpreted the results. BOV performed the essential oil extraction. ALD, WKC, AMO wrote the article. JCROFA, DMAFN performed the analysis and chemical characterization of essential oil. AMO, MVS performed a critical review of the final version of the article. MTSC, THN, AMO, MVS contributed reagents, materials and analytical tools.
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do Nascimento, A.L., Guedes, J.B., Costa, W.K. et al. Essential oil from the leaves of Eugenia pohliana DC. (Myrtaceae) alleviate nociception and acute inflammation in mice. Inflammopharmacol 30, 2273–2284 (2022). https://doi.org/10.1007/s10787-022-01067-y
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DOI: https://doi.org/10.1007/s10787-022-01067-y