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Genetic diversity analysis of pea (Pisum sativum l.) landraces by utilizing simple sequence repeat markers

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Abstract

The objective of current study was to differentiate among 46 pea genotypes collected from different areas of Azad Jammu and Kashmir and NARC Islamabad, Pakistan. We initially experienced 20 simple sequence repeat markers among those ten primers were selected based on high level of polymorphism. The average number of the bands score per primer were 29.75% whereas, average number of monomorphic and polymorphic bands were 16.25 and 12.85%, respectively. The genetic diversity among these genotypes ranged from 0.18 to 0.98. Maximum genetic diversity were noted in genotypes i.e., M-39, L29, L32, L1, L5, L8, L11, L24, L19, L17, L25, L23, L37 and M-83, while using cluster analysis based on dice coefficients as the unweighted pair group method with arithmetic mean (UPGMA). Furthermore, principal component (PC) analysis also showed similar results of UPGMA. The eight principal components (PC) accounted for 74.2% of the variation (21.74, 15.72, 8.76, 7.73, 6.19, 5.19, 4.50 and 4.37 for PC1, PC2, PC3, PC4, PC5, PC6, PC7 and PC8, respectively). The results of the present study will be useful in understanding the pea’s genetic makeup and in the selection of suitable landraces for a future pea crop improvement program.

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Acknowledgements

The researchers would like to acknowledge Deanship of Scientific Research, Taif University for funding this work. Authors are very thankful to the Department of Horticulture, The University of Poonch and National Agriculture Research Centre, Islamabad for providing funds and laboratory and field facilities for this study.

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No specific funding have been received for this study.

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Authors and Affiliations

  1. Department of Horticulture, University of the Poonch, Rawalakot, Pakistan

    Uzma Arif, Muhammad Jamil Ahmed & Ayaz Ahmed Arif

  2. National Agriculture Research Centre, Horticulture Research Institute, Islamabad, Pakistan

    Summaira Ali

  3. Department of Biology, University College of AlKhurmah, Taif University, Taif, Saudi Arabia

    Badr Alharthi

  4. POD, Ministry of National Food Security and Research, Islamabad, Pakistan

    Muhammad Farhan Khan Pasha

  5. Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan

    Hafiz Muhammad Ahmad

Authors
  1. Uzma Arif
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  2. Summaira Ali
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  3. Badr Alharthi
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  4. Muhammad Farhan Khan Pasha
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  5. Hafiz Muhammad Ahmad
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  6. Muhammad Jamil Ahmed
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  7. Ayaz Ahmed Arif
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Contributions

UA was the main author. MJA was supervising her. MAR was co supervisor and provided the lab facilities. AH has done the initial review of the manuscript. SZAS suggested some valid changes. AAA helped in data collection. IA carried out data analysis and interpretation. SH helped in manuscript writing and review and also in its publication.

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Correspondence to Uzma Arif or Hafiz Muhammad Ahmad.

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Arif, U., Ali, S., Alharthi, B. et al. Genetic diversity analysis of pea (Pisum sativum l.) landraces by utilizing simple sequence repeat markers. Genet Resour Crop Evol (2024). https://doi.org/10.1007/s10722-023-01796-1

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  • DOI: https://doi.org/10.1007/s10722-023-01796-1

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