Abstract
Plant height (PH) and main shoot length (MSL) are multi-allelic quantitative traits, that exert profound influences on crop productivity. The identification of QTLs responsible for PH and MSL is important for enhancing crop yield potential. To achieve this, we constructed a genetic linkage map using 192 SSRs in combination with 94 BCILs derived from JS1 and JS2 somatic hybrids. This comprehensive map spanned a total length of 1420.69 cM, expanding into 12 linkage groups. For PH, we identified a total of seven and one QTLs across two successive crop seasons. Interestingly, the minor QTLs identified during the first season exhibited relatively modest phenotypic effects (2.43–7.33%) while a dominant QTL (12.88% PVE) was identified in the subsequent crop season. All these QTLs displayed positive and negative additive effects, often accompanied by exceptionally high dominance effects (≤ 39.06). For MSL, we detected three QTLs during the first crop season, characterized by LOD values of 3.0063, 3.3156, and 8.9608, explaining 5.61%, 6.17%, and 16.27% PVE, respectively. Subsequently, two QTLs were identified on LG-2 with LOD values of 2.5869 (13.79% PVE) and 3.5740 (7.93% PVE), revealing negative additive effects. In the QTL regions for PH and MSL, we discovered a total of 20 genes, with eight and twelve genes associated with auxin and Brassinosteroids genes, displaying essential roles in regulating shoot height. In this pioneering study, we present the first-ever report detailing the identification of PH and MSL genes contributed by S. alba creating linkage drags in advanced BCILs and providing an opportunity for Brassica crop improvement.
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Abbreviations
- LOD:
-
logarithm of the odds
- PVE:
-
Phenotypic variance
- Add:
-
Additive Effect
- Dom:
-
Dominance Effect
- CI:
-
Confidence interval
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Funding
(1) Preetesh Kumari: Acknowledges the Department of Science and Technology (DST), Ministry of Science and Technology, New Delhi, Govt. of India, India for research grant [File No. SR/WOS-A/LS-373/2018(G)]; (2) Kaushal Pratap Singh received a fellowship (Research Associate) grant (File No. 09/1247(0001)/2019-EMR-I) from the HRDG-Council of Scientific and Industrial Research (HRDG-CSIR), New Delhi.
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PK: developed the somatic hybrids and their backcross progenies, conceived the study, fund acquisition, prepared the first draft and finalization of the manuscript; KPS conducted field trials for both years, phenotyping, field data analysis, genotyping, QTL mapping, editing, and finalization of the manuscript; PKR: Provided laboratory and field facilities to conduct this study, read the manuscript. All authors read the final version of the manuscript and approved it for submission.
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10681_2023_3271_MOESM2_ESM.xls
Details of SSR markers with SSR locations, left and right marker sequences, Tm, and product size used in the present study for QTL mapping (XLS 105.5 kb)
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Kumari, P., Singh, K.P. & Rai, P.K. Deciphering the genetic architecture of plant height and main shoot length in backcross introgression lines of Sinapis alba + Brassica juncea allohexaploids. Euphytica 220, 3 (2024). https://doi.org/10.1007/s10681-023-03271-5
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DOI: https://doi.org/10.1007/s10681-023-03271-5