Abstract
Watermelon possesses diverse ovary, fruit, and seed morphological traits and the successful identification of QTLs/genes controlling these traits are of great significance to breeders. Whole-genome re-sequencing using next-generation sequencing technology was employed to detect SNPs in the parental lines P1 (ZXG1553) and P2 (W1-17), for CAPS marker and linkage map development. The linkage map was developed by genotyping of 138 polymorphic CAPS markers with 191-F2 mapping population that covered all 11 chromosomes with a total length of 1853.34 cM. A total of thirty QTLs (2 Ovary weight, 2 Ovary length, 3 Ovary width, 4 Ovary hairiness, 2 Fruit weight, 1 Fruit length, 1 Fruit width, 1 Brix, 1 Rind thickness, 1 Seed length, 2 Seed width, 4 Rind chlorophyll a, 3 Rind chlorophyll b, and 3 Rind total chlorophyll) were detected for 14 significant ovary, fruit, and seed morphological traits. The QTLs exhibited different LOD score range (2.50 ~ 20.97) and phenotypic explained variance (3.30 ~ 25.91%) for all morphological traits. These detected QTLs and corresponding flanking CAPS markers could be valuable data to facilitate the further investigation in watermelon breeding programs and in-depth genetic basis studies.
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Acknowledgements
This work was supported by funding from the Taishan Industrial Leading Talents Project (LJNY202112) and China Agriculture Research System (CARS-25).
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Conceptualization: BAO, PG, FL; Methodology: HL, SL; Formal analysis and investigation: BAO, SA, AS, CZ, TL; Writing—original draft preparation: BAO; Writing—review and editing: PG, FL; Funding acquisition: FL; Resources: HL, SL; Supervision: PG, FL.
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Osae, B.A., Amanullah, S., Liu, H. et al. CAPS marker-base genetic linkage mapping and QTL analysis for watermelon ovary, fruit and seed-related traits. Euphytica 218, 39 (2022). https://doi.org/10.1007/s10681-022-02990-5
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DOI: https://doi.org/10.1007/s10681-022-02990-5