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Production and characterization of intergeneric hybrids by crossing radish with turnip and with Chinese kale

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Abstract

Distant hybridization can enrich the genetic background and diversity of crops by combining different species, and integrate the favorable parental traits or create heterosis. In the present study, intergeneric hybridization was performed between radish and turnip and between radish and Chinese kale by hand emasculation followed by tissue culture of putative hybrid seeds. A total of three and one hybrids were obtained from the cross of ‘Long yellow turnip’ and ‘Golden turnip’ with radish, respectively, but no hybrids were obtained in their reverse cross, indicating that the same cross combination has a large difference in the reciprocal crosses, and that the hybrid has certain unidirectionality. The majority of the hybrids of radish and turnip showed male sterility, white petals, and intermediate performance in most morphological traits when compared to their parents. Four true hybrids were obtained by the cross ‘Thin neck round radish’ × ‘Yellow flower kale’, while no hybrids were obtained by reverse cross combination. The hybrids looked more like their Chinese kale parents in having inflated stems. All putative intergeneric hybrids were confirmed by agronomic characteristics, chromosome counting, molecular markers, and pollen viability. Three kinds of hybrids were confirmed as genuine hybrids possessing 19, 19, and 18 chromosomes in their corresponding somatic cells by cytological observation. In addition, three kinds of allotetraploids were produced with 38, 38, and 36 chromosomes when chromosome doubling was achieved. In comparison with the diploid hybrid, the pollen viabilities of allotetraploids were significantly improved and a small number of seeds were produced. The improvements of these amphihaploids as useful genetic resources and the potential of intergeneric hybridization to enlarge the gene pool for radish, turnip, and Chinese kale breeding are discussed.

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References

  • Chen HF, Wang H, Li ZY (2007) Production and genetic analysis of partial hybrids in intertribal crosses between Brassica species (B. rapa, B. napus) and Capsella bursa-pastoris. Plant Cell Rep 26(10):1791–1800

    Article  CAS  PubMed  Google Scholar 

  • Chen HG, Wu JS (2008) Characterization of fertile amphidiploid between Raphanus sativus and Brassica alboglabra and the crossability with Brassica species. Genet Resour Crop Evol 55(1):143–150

    Article  CAS  Google Scholar 

  • Crute IR, Gray AR, Crisp P, Buczacki ST (1980) Variation in Plasmodiophora brassicae and resistance to clubroot disease in Brassicas and allied crops—a critical review. Plant Breed 50:91–104

    Google Scholar 

  • Han JM, Hou XL, Xu HM, Shi GJ, Wang JJ (2008) RAPD analysis of genetic diversity of non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) germplasm. J Nanjing Agric Univ 31(3):31–36

    CAS  Google Scholar 

  • Hansen LN, Earle ED (1997) Somatic hybrids between Brassica oleracea L and Sinapis alba L. with resistance to Alternaria brassicae (Berk.) Sacc. Theor Appl Genet 94(8):1078–1085

    Article  Google Scholar 

  • Hu DY, Wang AY, Li X (2006) Research on the compatibility of distant hybridization between radish, Brassica napus and Brassica nigra. Crop Res 20(2):124–126

    Google Scholar 

  • Hu Q, Li YC, Mei DS, Fang XP (2004) Establishment and identification of cytoplasmic male sterility in Brassica napus by intergeneric somatic hybridization. Sci Agric Sin 37(3):333–338

    Google Scholar 

  • Inomata N (1993) Crossability and cytology of hybrid progenies in the cross between Brassica campestris and three wild relatives of B. oleracea, B. bourgeaui, B. cretica and B. montana. Euphytica 69(1–2):7–17

    Article  Google Scholar 

  • Jarl CI, van Grinsven MQJM, van den Mark F (1989) Correction of chlorophyll-defective male-sterile winter oilseed rape (Brassica napus) through organelle exchange: molecular analysis of the cytoplasm of parental lines and corrected progeny. Theor Appl Genet 77:135–141

    Article  CAS  PubMed  Google Scholar 

  • Jeong YM, Kim N, Ahn BO, Oh M, Chung WH et al (2016) Elucidating the triplicated ancestral genome structure of radish based on chromosome-level comparison with the Brassica genomes. Theor Appl Genet 129(7):1357–1372

    Article  CAS  PubMed  Google Scholar 

  • Jiang L, Li D, Jin L, Ruan Y, Shen WH, Liu C (2018) Histone lysine methyltransferases BnaSDG8.A and BnaSDG8.C are involved in the floral transition in Brassica napus. Plant J 95:672–685

    Article  CAS  Google Scholar 

  • Leitch AR, Leitch IJ (2008) Genomic plasticity and the diversity of polyploid plants. Science 320:481–483

    Article  CAS  PubMed  Google Scholar 

  • Li G, Li F, Zhang S, Zhang H, Zhang S, Sun R (2019) Cloning and function analysis of a novel flowering time regulatory gene BraELF6 in Brassica rapa. Sci Hortic 248:126–131

    Article  CAS  Google Scholar 

  • Li JJ, Ding Q, Wang FD, Zhang YH, Li HY, Gao JW (2015a) Integrative analysis of mRNA and miRNA expression profiles of the tuberous root development at seedling stages in turnips. PLoS ONE 10(9):e0137983

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Li JX, Rao LL, Meng QF, Ghani MA, Chen LP (2015b) Production of Brassica tri-genomic vegetable germplasm by hybridisation between tuber mustard (Brassica juncea) and red cabbage (B. oleracea). Euphytica 204(2):323–333

    Article  Google Scholar 

  • Li RX, Li JN (2015) Differential expression of genes in diploid, tetraploid and synthesized polyploids of Brassica. J Southwest Univ (Nat Sci Ed) 37(6):27–34

    Google Scholar 

  • Li XF (1995) Research progress in distant hybridization between Raphanus and Brassica. Chin J Oil Crop Sci 17(1):69–73

    CAS  Google Scholar 

  • Li XL, Guo WW, Wang B, Li XS, Chen HG, Wei LH, Wang YJ, Wu JS, Long H (2010) Instability of chromosome number and DNA methylation variation induced by hybridization and amphidiploid formation between Raphanus sativus L. and Brassica alboglabra Bailey. BMC Plant Biol 10(1):207

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Lim SJ, Lee SS, Bang JW (2012) Karyotype and genomic in situ hybridization pattern in × Brassicoraphanus, an intergeneric hybrid between Brassica campestris ssp. pekinensis and Raphanus sativus. Plant Biotechnol Rep 6(2):107–112

    Article  Google Scholar 

  • Liu YP, Xu AX, Liang FH, Yao XQ, Wang Y, Liu X, Zhang Y et al (2018) Screening of clubroot-resistant varieties and transfer of clubroot resistance genes to Brassica napus using distant hybridization. Breed Sci 68(2):258–267

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu MY, Basseti N, Petrasch S, Zhang NW, Bucher J, Shen SX, Zhao JJ, Bonnema G (2019) What makes turnips: anatomy, physiology and transcriptome during early stages of its hypocotyl-tuber development. Hortic Res 6:38

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Lou LN, Lou QF, Li ZA, Xu YY, Liu Z, Su XJ (2017) Production and characterization of intergeneric hybrids between turnip (Brassica rapa L. em. Metzg. subsp. rapa) and radish (Raphanus sativus L.). Sci Hortic 220:57–65

    Article  Google Scholar 

  • Mei J, Ding Y, Lu K, Wei D, Liu Y, Disi JO, Li J, Liu L, Liu S, McKay J, Qian W (2013) Identification of genomic regions involved in resistance against Sclerotinia sclerotiorum from wild Brassica oleracea. Theor Appl Genet 126(2):549–556

    Article  CAS  PubMed  Google Scholar 

  • Meng J, Shi S, Gan L, Li Z, Qu X (1998) The production of yellow-seeded Brassica napus (AACC) through crossing interspecific hybrids of B. campestris (AA) and B. carinata (BBCC) with B. napus. Euphytica 103:329–333

    Article  Google Scholar 

  • Mohammed AE, You MP, Banga SS, Surinder SB, Barbetti MJ (2019) Resistances to downy mildew (Hyaloperonospora brassicae) in diverse Brassicaceae offer new disease management opportunities for oilseed and vegetable crucifer industries. Eur J Plant Pathol 153:915–929

    Article  CAS  Google Scholar 

  • Paulmann W, Robbelen G (1988) Effective transfer of cytoplasmic male sterility from radish (Raphanus sativus L.) to rape (Brassica napus L.). Plant Breed 100(4):299–309

    Article  Google Scholar 

  • Peterka H, Budahn H, Schrader O, Ahne R, Schütze W (2004) Transfer of resistance against the beet cyst nematode from radish (Raphanus sativus) to rape (Brassica napus) by monosomic chromosome addition. Theor Appl Genet 109(1):30–41

    Article  CAS  PubMed  Google Scholar 

  • Pires JC, Zhao J, Schranz ME, Leon EJ, Quijada A, Lukens LN, Osborn TC (2004) Flowering time divergence and genomic rearrangements in resynthesized Brassica polyploids (Brassicaceae). Biol J Linn Soc 82(4):675–688

    Article  Google Scholar 

  • Schranz ME, Quijada PA, Sung SB, Lukens L, Amasino R, Osborn TC (2002) Characterization and effects of the replicated flowering time gene FLC in Brassica rapa. Genetics 162(3):1457–1468

    CAS  PubMed  PubMed Central  Google Scholar 

  • Singh S, Dey SS, Bhatia R, Kumar R, Behera TK (2019) Current understanding of male sterility systems in vegetable Brassicas and their exploitation in hybrid breeding. Plant Reprod 32(3):231–256

    Article  PubMed  Google Scholar 

  • Snowdon RJ, Winter H, Diestel A, Sacristán MD (2000) Development and characterisation of Brassica napus-Sinapis arvensis addition lines exhibiting resistance to Leptosphaeria maculans. Theor Appl Genet 101(7):1008–1014

    Article  Google Scholar 

  • Sun CZ, Li Y, Zhang SN, Zheng JS (2014) Production of intergeneric allotetraploid between autotetraploid non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) and autotetraploid radish (Raphanus sativus L.). Acta Soc Bot Pol 83(1):75–79

    Article  CAS  Google Scholar 

  • Takeshita M, Kato M, Tokumasu S (1980) Application of ovule culture to the production of intergeneric or interspecific hybrids in Brassica and Raphanus. Jpn J Genet 55(5):373–387

    Article  Google Scholar 

  • Wang AY, Zhang XH, Yang CH, Song ZJ, Du CQ, Chen DL, He YC, Cai DT (2013) Development and characterization of synthetic amphiploid (AABB) between Oryza sativa and Oryza punctata. Euphytica 189(1):1–8

    Article  Google Scholar 

  • Wang GX, Lv J, Zhang J, Han S, Zong M, Guo N, Zeng XY, Zhang YY, Wang YP, Liu F (2016) Genetic and epigenetic alterations of Brassica nigra introgression lines from somatic hybridization: a resource for cauliflower improvement. Front Plant Sci 7:1258

    PubMed  PubMed Central  Google Scholar 

  • Wu JG, Li ZY, Liu Y, Shi CH, Fu TT (1999) A cytological method for determining wide hybrid of rapeseed. Hereditas 21(5):51–52

    Google Scholar 

  • Yang XL, Tao FF, Peng Y, He SM (2016) Studies on the heterosis of distant hybridization of Brassica crops. Crop Res 30(5):520–523

    Google Scholar 

  • Yu N, Cao L, Yuan L, Zhi X, Chen Y, Gan S, Chen L (2018) Maintenance of grafting-induced epigenetic variations in the asexual progeny of Brassica oleracea and B. juncea chimera. Plant J 96(1):22–38

    Article  CAS  PubMed  Google Scholar 

  • Zhan ZX, Nwafor CC, Hou ZK, Gong JF, Zhu B, Yi Jiang YF, Zhou YM, Wu JS, Piao ZY, Tong Y, Liu C, Zhang CY (2017) Cytological and morphological analysis of hybrids between Brassicoraphanus and Brassica napus for introgression of clubroot resistant trait into Brassica napus L. PLoS ONE 12(5):e0177470

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Zhang DS, Zhang N, Yu SC, Zhang FL, Sui FL, Yu YJ et al (2014) Genetic diversity of Chinese kale based on SSR markers. Acta Agric Boreali Sin 29(4):140–144

    Google Scholar 

  • Zhang L, Zhao H, Chen B, Liu F (2008) Development and identification of interspecific somatic hybrids between cauliflower and black mustard. Chin Bull Bot 25(2):176–184

    CAS  Google Scholar 

  • Zhou FJ (2006) The hybrids of intergeneric hybridization between Brassica species and Raphanus sativus and their identification by SSR. Doctoral dissertation, Hunan Agricultural University, China

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Acknowledgements

This research was financed by the Program for Modern Seed Industrial Developing Engineer of Zhejiang Province (2015004) and the Three Rural Areas and Six Parties program of Zhejiang Province (2045210244), China. The English of the paper is improved by Dr. Ross Ferguson.

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  1. Ping Jin and Zhiyu Zhu have contributed equally to this work.

Authors and Affiliations

  1. College of Agriculture and Food Science, Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Zhejiang A&F University, Wusu Street 666, Lin’an, Hangzhou, 311300, Zhejiang, People’s Republic of China

    Ping Jin, Zhiyu Zhu, Xiaocan Guo, Fangfang Chen, Yuanyuan Wu, Jianguo Wu & Zhujun Zhu

  2. Department of Health and Agriculture, Hangzhou Wanxiang Polytechnic, Huajiang Road 3, Hangzhou, 310023, Zhejiang, People’s Republic of China

    Jiahong Chen

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  1. Ping Jin
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Jin, P., Zhu, Z., Guo, X. et al. Production and characterization of intergeneric hybrids by crossing radish with turnip and with Chinese kale. Euphytica 216, 90 (2020). https://doi.org/10.1007/s10681-020-02622-w

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