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Marker-assisted improvement of grain protein content and grain weight in Indian bread wheat

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Abstract

The leading Indian wheat cultivar ‘HUW234’ produces grain with relatively low protein content (GPC) and thousand grain weight (TGW). Therefore, marker-assisted backcrossing was used to improve these two important traits by introducing favorable genes/alleles from cv. ‘Glu133’, which harbors both alleles for both GPC (Gpc-B1) and TGW. Foreground selection for GPC was achieved using microsatellite markers Xucw108 linked with Gpc-B1 and Xgwm297 linked with TGW. Background selection applied to support recovery of the recurrent parent genotype was based on 86 genomically distributed microsatellites. A selected BC2F1 plant was the progenitor of 15 BC2F4 families, in which representation of the cv. HUW234 genome ranged from 89.5 to 93.0 %. The new derivatives of HUW234 were comparable in yield to the original cultivar, but with both significantly higher GPC and heavier kernels.

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Acknowledgments

We gratefully acknowledge partial funding from a DBT, New Delhi Network Project and a CSISA - Wheat Breeding project funded by the Bill and Melinda Gates Foundation and USAID for field based support. We sincerely thank Prof. Pushpendra K. Gupta, Ch. Charan Singh University, Meerut for sustained intellectual support. Use of the Indian Agriculture Research Institute off-season facility at Regional Station, Wellington, Tamil Nadu is gratefully acknowledged. We thank Dr. Parveen Chuneja (Punjab Agriculture University, Ludhiana, India) for supplying grain of line Glu133 and Dr. R. R. Mir (S. K. University of Agricultural Sciences and Technology, Jammu, India) for providing TGW marker information.

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Authors and Affiliations

  1. Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India

    Manish K. Vishwakarma, B. Arun, V. K. Mishra, P. S. Yadav, H. Kumar & Arun K. Joshi

  2. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India

    Manish K. Vishwakarma

  3. CIMMYT, South Asia Regional Office, Singha Durbar Plaza Road, Kathmandu, Nepal

    Arun K. Joshi

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  1. Manish K. Vishwakarma
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  2. B. Arun
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  3. V. K. Mishra
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  4. P. S. Yadav
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  5. H. Kumar
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  6. Arun K. Joshi
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Correspondence to Arun K. Joshi.

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Vishwakarma, M.K., Arun, B., Mishra, V.K. et al. Marker-assisted improvement of grain protein content and grain weight in Indian bread wheat. Euphytica 208, 313–321 (2016). https://doi.org/10.1007/s10681-015-1598-6

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  • DOI: https://doi.org/10.1007/s10681-015-1598-6

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