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Bioconversion of vitamin D3 to calcifediol by using resting cells of Pseudonocardia sp.

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Abstract

Objectives

Resting cells of Pseudonocardia sp. KCTC 1029BP were used for the bioconversion of vitamin D3 to calcifediol which is widely used to treat osteomalacia and is industrially produced by chemical synthesis.

Results

To obtain the maximum bioconversion yield of calcifediol by the microbial conversion of vitamin D3, a two-step optimization process was used, including the Plackett–Burman and the central composite designs. Six variables, namely agitation speed, aeration rate, resting cell concentration, vitamin D3 concentration, temperature, and pH, were monitored. Of these, aeration rate, resting cell concentration, and temperature were selected as key variables for calcifediol production and were optimized using the central composite design. Optimal bioconversion conditions obtained were as follows: aeration rate of 0.2 vvm, resting cell concentration of 4.7 % w/v, and temperature of 33 °C.

Conclusion

Using the optimal conditions, 356 mg calcifediol l−1 was obtained with a bioconversion yield of 59.4 % in a 75 l fermentor. These are the highest values reported to date.

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Acknowledgments

KHK acknowledges the Grant support by the Ministry of Trade, Industry & Energy (10048684) and the facility support at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety.

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Authors and Affiliations

  1. Department of Biotechnology, Graduate School, Korea University, Seoul, 136-713, Republic of Korea

    Dae-Jung Kang & Kyoung Heon Kim

  2. Research Laboratories, Ildong Pharmaceutical, Hwaseong, 445-170, Republic of Korea

    Dae-Jung Kang, Jong-Hyuk Im & Jae-Hoon Kang

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  1. Dae-Jung Kang
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  2. Jong-Hyuk Im
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  3. Jae-Hoon Kang
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  4. Kyoung Heon Kim
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Correspondence to Kyoung Heon Kim.

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Kang, DJ., Im, JH., Kang, JH. et al. Bioconversion of vitamin D3 to calcifediol by using resting cells of Pseudonocardia sp.. Biotechnol Lett 37, 1895–1904 (2015). https://doi.org/10.1007/s10529-015-1862-9

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  • DOI: https://doi.org/10.1007/s10529-015-1862-9

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