Abstract
We assessed the ability of aPseudonocardia sp. from soil samples to bioconvert vitamin D3. The optimal culture conditions for the bioconversion of vitamin D3 to active 1α,25-dihydroxyvitamin D3 were investigated by varying the carbon and nitrogen sources, the metal salt concentrations, the initial pH, and the temperature. Microbial transformations were carried out with the addition of vitamin D3 dissolved in ethanol. They were sampled by extraction with methanol-dichloromethane and the samples were examined by HPLC. Optimum culture conditions were found to be 0.4% yeast extract, 1% glucose, 3% starch, 1% fish meal, 0.2% NaCl, 0.01% K2HPO4, 0.2% CaCO3, 0.01% NaF, and pH 7.0 at 28°C. The optimal timing of the addition of vitamin D3 for the production of calcitriol byPseudonocardia autotrophica ID 9302 was concurrent with the inoculation of seed culture broth. Maximum calcitriol productivity and the yield of bioconversion reached a value of 10.4 mg/L and 10.4% respectively on the 7th day in a 75 L fermenter jar under the above conditions.
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References
DeLuca, H. F., and H. K. Schnoes (1983) Vitamin D: recent advances.Annu. Rev. Biochem. 52: 411–439.
Madhok, T. C., and H. F. DeLuca (1979) Characteristics of the rat liver microsomal enzyme system converting cholecalciferol into 25-hydroxycholecalciferol Evidence for the participation of cytochrome p-450.Biochem. J. 184: 491–499.
Ikekawa, N. (1987) Structures and biological activities of vitamin D metabolites and their analogs.Med. Res. Rev. 7: 333–366.
Brenza, H. L., and H. F. DeLuca (2000) Regulation of 25-hydroxyvitamin D3 1α-hydroxylase gene expression by parathyroid hormone and 1,25-dihydroxyvitamin D3 Arch. Biochem. Biophys. 381: 143–152.
Xue, Y., A. C. Karaplis, G. N. Hendy, D. Goltzman. and D. Miao (2005) Genetic models show that parathyroid hormone and 1,25-dihydroxyvitamin D3 play distinct and synergistic roles in postnatal mineral ion homeostasis and skeletal development.Hum Mol. Genet. 14: 1515–1528.
Beckman, M. J. and H. F. DeLuca (2002) Regulation of renal vitamin D receptor is an important determinant of 1α,25-dihydroxyvitamin D3, levelsin vivo.Arch. Biochem. Biophys. 401: 44–52.
Hatakeyama, S., K. Sugawara, H. Numata, and S. Takano (1991) A novel convergent synthesis of (+)-1α,25-dihydroxyvitamin D3 using a chromium(II)-mediated coupling reaction.J. Org. Chem. 56: 461–463.
Andrews, D. R., D. H. R. Barton, R. H. Hesse, and M. M. Pechet (1986) Synthesis of 25-hydroxy- and 1α,25-dihydroxyvitamin D3 from vitamin D2 (calciferol).J. Org. Chem. 51: 4819–4828.
Kametani, T., and H. Furuyama (1987) Synthesis of vitamin D3 and related compounds.Med. Res. Rev. 7: 147–171.
Wang, Z. X., J. Zhuge, H. Fang, and B. A. Prior (2001) Glycerol production by microbial fermentation: a review.Biotechnol. Adv. 19: 201–223.
Lau, J., C. Tran, P. Licari, and J. Galazzo (2004) Development of a high cell-density fed-batch bioprocess for the heterologous production of 6-deoxythronolide B inEscherichia coli.J. Biotechnol. 110: 95–103.
Flores, E. R., F. Perez, and M. De La Torre (1997) ScaleupBacillus thuringensis fermentation based on oxygen transfer.J. Ferment. Bioeng. 83: 561–564.
Uzura, A., T. Katsuragi, and Y. Tani (2001) Optimal conditions for production of (R)-1-phenylpropanol byFusarium moniliforme strain MS31.J. Biosci. Bioeng. 92: 288–293.
Kulprecha, S., T. Ueda, T. Nihira, T. Yoshida, and H. Taguchi (1985) Optimum conditions for ursodeoxycholic acid production from lithocholic acid byFusarium equiseti M41.Appl. Environ. Microbiol. 49: 338–344.
Wecht-Lifshitz, S. C., M. Gadman, and E. Zomer (1989) Process optimization and scale-up of theBacillus thuringiensis fermentation.Isr. J. Entomol. 23: 239–246.
Lund, J., and H. F. DeLuca (1966) Biologically active metabolite of vitamin D3 from bone, liver, and blood serum,J. Lipid Res. 7: 739–744.
Omar, R., M. A. Abdullah, M. A. Hasan, M. Marziah, and M. K. Siti Mazlina (2005) Optimization and elucidation of interactions between ammonium, nitrate and phosphate inCentella asiatica cell culture using response surface methodology.Biotechnol. Bioprocess Eng. 10: 192–197.
Jung, D.-Y., S. Jung, J.-S. Yung, J.-N. Kim, Y.-J. Wee, H.-G. Jang, and H.-W. Ryu (2005) Influences of cultural medium component on the production of poly(γ-glutamic acid) byBacillus sp. RKY3.Biotechnol. Bioprocess Eng. 10: 289–295.
Sasaki, J., A. Mikami, K. Mizoue, and S. Omura (1991) Transformation of 25- and 1α-hydroxyvitamin D3 to 1α,25-dihydroxyvitamin D3 by usingStreptomyces sp. strains.Appl. Environ. Microbiol. 57: 2841–2846.
Hiwatashi, A., Y. Nishii, and Y. Ichikawa (1982) Purification of cytochrome P-450D1 α (25-hydroxyvitamin D3-1α-hydroxylase) of bovine kidney mitochondira.Biochem. Biophys. Res. Commun. 105: 320–327.
Kizawa, H., D. Tomura, M. Oda, A. Fukamizu, T. Hoshino, O. Gotoh, T. Yasui, and H. Shoun (1991) Nucleotide sequence of the unique nitrate/nitrite-inducible cytochrome P-450 cDNA fromFusarium oxysporum, J. Biol. Chem. 266: 10632–10637.
Oh, Y.-K., Y.-J. Kim, J.-Y. Park, T. H. Lee, M.-S. Kim, and S. Park (2005) Biohydrogen production from carbon monoxide and water byRhodopseudomonas palustris P4Biotechnol. Bioprocess Eng. 10: 270–274.
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Kang, DJ., Lee, HS., Park, JT. et al. Optimization of culture conditions for the bioconversion of vitamin D3 to 1α,25-dihydroxyvitamin D3 usingPseudonocardia autotrophica ID 9302. Biotechnol. Bioprocess Eng. 11, 408–413 (2006). https://doi.org/10.1007/BF02932307
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DOI: https://doi.org/10.1007/BF02932307