Abstract
The effects of cultivation medium compositions including soybean meal, peptone, soybean oil and cornstarch for actinomycin X2 production by Streptomyces spp JAU4234 were accessed by using response surface methodology. The 24 full factorial designs and the paths of steepest ascent were effective in searching for the major factors of actinomycin X2 production. In this study, cornstarch and soybean oil showed negative effect on actinomycin X2 production based on the first-order regression coefficients derived from MINITAB software. Subsequently, a central composite design for optimization was further investigated. Preliminary studies showed that soybean meal and peptone were believed to be the major factors for actinomycin X2 production. Estimated optimum compositions for the production of actionmycin X2 were as follows (g/l): soybean meal 21.65 and peptone 9.41, and result in a maximum actionmycin X2 production of 617.4 mg/l. This value was closed to the 612 mg/l actionmycin X2 production from actual experimental observations. The yield of actionmycin X2 was increased by 36.9% by culturing the strain Streptomyces spp JAU4234 in the nutritionally optimized fermentation medium.
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This research was support by the Natural Science Foundation of Jiangxi Province, People’s Republic of China (Project no. 050010). The authors also express their appreciation to Dr. Yuhui Sun (Department of Biochemistry, University of Cambridge, UK) for revising this manuscript.
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Xiong, ZQ., Tu, XR. & Tu, GQ. Optimization of medium composition for actinomycin X2 production by Streptomyces spp JAU4234 using response surface methodology. J Ind Microbiol Biotechnol 35, 729–734 (2008). https://doi.org/10.1007/s10295-008-0339-4
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DOI: https://doi.org/10.1007/s10295-008-0339-4