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Genetic Diversity in Various Accessions of Pineapple [Ananas comosus (L.) Merr.] Using ISSR and SSR Markers

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Abstract

Inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers were used to assess the genetic diversity of 36 pineapple accessions that were introduced from 10 countries/regions. Thirteen ISSR primers amplified 96 bands, of which 91 (93.65%) were polymorphic, whereas 20 SSR primers amplified 73 bands, of which 70 (96.50%) were polymorphic. Nei’s gene diversity (h = 0.28), Shannon’s information index (I = 0.43), and polymorphism information content (PIC = 0.29) generated using the SSR primers were higher than that with ISSR primers (h =  0.23, I = 0.37, PIC = 0.24), thereby suggesting that the SSR system is more efficient than the ISSR system in assessing genetic diversity in various pineapple accessions. Mean genetic similarities were 0.74, 0.61, and 0.69, as determined using ISSR, SSR, and combined ISSR/SSR, respectively. These results suggest that the genetic diversity among pineapple accessions is very high. We clustered the 36 pineapple accessions into three or five groups on the basis of the phylogenetic trees constructed based on the results of ISSR, SSR, and combined ISSR/SSR analyses using the unweighted pair-group with arithmetic averaging (UPGMA) method. The results of principal components analysis (PCA) also supported the UPGMA clustering. These results will be useful not only for the scientific conservation and management of pineapple germplasm but also for the improvement of the current pineapple breeding strategies.

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Acknowledgements

The authors gratefully acknowledge Sezai Ercisli (Ataturk University Agricultural Faculty Department of Horticulture 25240 Erzurum, Turkey DRARO) for revising this manuscript. The Central Level Scientific Research Unit Public Welfare Project of China (Grant No. 1630032013025) and Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (17CXTD10) supported this project.

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  1. Jian-sheng Wang and Jun-hu He contributed equally to this work and they are co-first authors.

Authors and Affiliations

  1. Tropical Crops Genetic Resources Institute of Chinese Academy of Tropical Agricultural Science, Danzhou, 571737, Hainan Province, China

    Jun-hu He, Hua-rui Chen, Ye-yuan Chen & Fei Qiao

  2. Pingdingshan University, Pingdingshan, 467000, Henan Province, China

    Jian-sheng Wang

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  1. Jian-sheng Wang
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Correspondence to Jun-hu He.

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Wang, Js., He, Jh., Chen, Hr. et al. Genetic Diversity in Various Accessions of Pineapple [Ananas comosus (L.) Merr.] Using ISSR and SSR Markers. Biochem Genet 55, 347–366 (2017). https://doi.org/10.1007/s10528-017-9803-z

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  • DOI: https://doi.org/10.1007/s10528-017-9803-z

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