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Analysis of genetic diversity of Ruthenia Medic (Medicago ruthenica (L.) Trautv.) in Inner Mongolia using ISSR and SSR markers

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Abstract

Ruthenia Medic is tolerant to drought, cold, high salinity, resistance to trampling and high quality features. Inter-simple sequence repeat (ISSR) and simple sequence repeat (SSR) molecular markers were employed for the first time to access the genetic diversity and relationships of 30 wild Ruthenia Medic accessions obtained from Inner Mongolia in the present study. A total of 94 bands were amplified by ten ISSR primers, of which 83 (88.5 %) were polymorphic, and 57 polymorphic bands (80.4 %) were observed in 69 bands amplified by ten SSR primers. Shannon’s information index (I = 0.487), and average expected heterozygosis (He = 0.329) generated by ISSR primer were higher than that of SSR analysis (I = 0.372, He = 0.231). The study indicated that ISSR were more effective than SSR markers for assessing the degree of genetic variation of Ruthenia Medic. UPGMA cluster analysis revealed inconsistencies in the clustering patterns, as the Mantel’s test between the dendrograms for ISSR and SSR data indicated a poor fit for the ISSR and SSR data types (r = 0.0970). Whereas the pattern of clustering of the genotypes remained relatively the same in ISSR and combined data of ISSR and SSR. The results of principal components analysis also supports their UPGMA clustering. These results have an important implication for Ruthenia Medic germplasm characterization, improvement, and conservation.

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Acknowledgments

This study was supported by Ministry of Agriculture, Crop Germplasm Resources Protection and Utilization Project (NB2012-2130135-33), Ministry of Agriculture Forage Germplasm Resources Protection Program (2012-43). The authors wish to thanks an anonymous reviewer for his/her valuable suggestions on the manuscript.

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Authors and Affiliations

  1. The Institute of Grassland Research (CAAS)/SharaQin Key Wild Scientific Monitoring Station for Forage Resources of Ministry of Agriculture, Inner Mongolia, Hohhot, 010010, China

    Hong-yan Li, Zhi-yong Li, Li-yan Cai & Wen-gui Shi

  2. Inner Mongolia Agricultural University, Hohhot, 010010, China

    Fu-gui Mi & Feng-ling Shi

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  1. Hong-yan Li
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  2. Zhi-yong Li
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  4. Wen-gui Shi
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  5. Fu-gui Mi
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  6. Feng-ling Shi
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Correspondence to Zhi-yong Li.

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Li, Hy., Li, Zy., Cai, Ly. et al. Analysis of genetic diversity of Ruthenia Medic (Medicago ruthenica (L.) Trautv.) in Inner Mongolia using ISSR and SSR markers. Genet Resour Crop Evol 60, 1687–1694 (2013). https://doi.org/10.1007/s10722-012-9950-3

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  • DOI: https://doi.org/10.1007/s10722-012-9950-3

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