Single injection of muscarinic cholinoceptor blocker atropine (1 mg/kg) to outbred male rats reduced β-adrenergic responsiveness of erythrocytes (by 2.2 times) and the content of epinephrine granules on erythrocytes (by 1.5 times), significantly increased HR and rigidity of the heart rhythm, and manifold decreased the power of all spectral components of heart rhythm variability. Stimulation of the central neurotransmitter systems increased β-adrenergic responsiveness of erythrocytes (by 15-26%), decreased the number of epinephrine granules on erythrocytes (by 25-40%), and increased HR and cardiac rhythm intensity. These changes were most pronounced after stimulation of the serotoninergic system. Administration of atropine against the background of activation of central neurotransmitter systems did not decrease β-adrenergic responsiveness of erythrocytes (this parameter remained at a stably high level and even increased during stimulation of the dopaminergic system), but decreased the number of epinephrine granules on erythrocytes, increased HR, and dramatically decreased the power of all components of heart rhythm variability spectrum. The response to atropine was maximum against the background of noradrenergic system activation and less pronounced during stimulation of the serotoninergic system. Thus, substances that are complementary to cholinergic receptors modulated adrenergic effect on the properties of red blood cells, which, in turn, can modulate the adrenergic influences on the heart rhythm via the humoral channel of regulation. Stimulation of central neurotransmitter systems that potentiates the growth of visceral adrenergic responsiveness weakens the cholinergic modulation of the adrenergic influences, especially with respect to erythrocyte responsiveness. Hence, changes in the neurotransmitter metabolism in the body can lead to coupled modulation of reception and reactivity to adrenergic- and choline-like regulatory factors at the level of erythrocyte membranes, which can be important for regulation of heart rhythm.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Artemova EV, Gorbacheva AM, Galstyan GR, Tokmakova AY, Gavrilova SA, Dedov II. Neurohumoral mechanisms of keratinocytes regulation in diabetes mellitus. Sakh. Diabet. 2016;19(5):366-374. Russian.
Baevskii RM, Ivanov GG, Chireikin LV, Gavrilushkin AP, Dovgalevskii PYa, Kukushkin YuA, Mironova TF, Prolutskii DA, Semenov AV, Fedorov VF, Fleishman AN, Medvedev MM. Analysis of heart rhythm variability using different electrocardiographic systems (part 1). Vestn. Aritmol. 2002;(24):65-86. Russian.
Bilalova GA, Kazanchikova LM, Zefirov TL, Sitdikov FG. Inotropic effect of dopamine on rat heart during postnatal ontogeny. Bull. Exp. Biol. Med. 2013;156(2):173-176.
Dygai AM1, Skurikhin EG. Monoaminergic regulation of hemopoiesis under extreme conditions. Bull. Exp. Biol. Med. 2011;151(2):171-178.
Kuryanova EV, Zhukova YD, Tryasuchev AV, Horst NA. Influence of scopolamine, galantamine and their combination with hexametonium and atropine on the spectral characteristics of heart rhythm of nonlinear rats. Sib. Nauch. Med. Zh. 2016;36(3):5-12. Russian.
Kur’yanova EV, Tryasuchev AV, Stupin VO, Teplyi DL. Effect of Stimulation of Neurotransmitter Systems on Heart Rate Variability and β-Adrenergic Responsiveness of Erythrocytes in Outbred Rats. Bull. Exp. Biol. Med. 2017;163(1):31-36.
Manukhin BN, Nesterova LA. Allosteric influence of atropine and carbachol on activity of membranes 2-adrenoreceptors in rat cerebral cortex. Biol. Membrany. 2011;28(5):382-386. Russian.
Manukhin BN, Nesterova LA. Allosteric effect of serotonin and mianserin on the kinetics of specific [3H]-ligand binding to adrenergic and muscarinic receptors in the rat cerebral cortex membranes. Biol. Bull. 2015;42(2):129-138.
Astaf’eva OG, Vilkova EE. Patent USSR No. 1730555. A method of cytological detection of catecholamines in erythrocytes. 1992. Bull. No. 16. Russian.
Sergeeva OV, Alipov NN, Smirnov VM. Effect of atropine, propranolol, and atenolol on wave structure of heart rate oscillations in rats. Bull. Exp. Biol. Med. 2008;145(4):387-390.
Stryuk RI, Dlusskaya IG. Adrenoreactivity and Cardiovascular System. Moscow, 2003. Russian.
Tsirkin VI, Anisimov KY, Khlybova SV, Dmitrieva SL, Bratukhina OA, Popova VS, Shushkanova EG. Chemoreactivity of erythrocytes as indicators of pregnancy and labor (review). Vestn. Ural. Med. Akad. Nauki. 2015;(4):143-150. Russian.
Basic and Clinical Pharmacology. Katzung BG, Masters SB, Trevor AJ, eds. New York, 2012.
Henze M, Tiniakov R, Samarel A, Holmes E, Scrogin K. Chronic fluoxetine reduces autonomic control of cardiac rhythms in rats with congestive heart failure. Am. J. Physiol. Heart Circ. Physiol. 2013;304(3):H444-H454.
Spasojevic N, Gavrilovic L, Kovacevic I, Dronjak S. Effects of antidepressants maprotiline and fluxilan on sympathoadrenomedullary system in stressed rats. Auton. Neurosci. 2009;145(1-2):104-107.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 5, pp. 536-540, May, 2018.
Rights and permissions
About this article
Cite this article
Kur’yanova, E.V., Tryasuchev, A.V., Stupin, V.O. et al. Effect of Atropine on Adrenergic Responsiveness of Erythrocyte and Heart Rhythm Variability in Outbred Rats with Stimulation of the Central Neurotransmitter Systems. Bull Exp Biol Med 165, 597–601 (2018). https://doi.org/10.1007/s10517-018-4221-8
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-018-4221-8