Abstract
A novel cellulolytic strain JC656T was isolated from the rhizosphere soil of Alisma plantago-aquatica of floating island (Phumdis) of Loktak lake, Manipur, India. The 16S rRNA gene sequence similarities between strain JC656T and other Sinomonas type strains ranged between 98.5 and 97.3%, wherein strain JC656T exhibited the highest sequence similarity (98.5%) to Sinomonas notoginsengisoli KCTC 29237T. Colonies were yellow-colored and grew aerobically. Cells were gram-positive, rod-shaped and non-motile. The optimal growth of the strain JC656T occured at 28 °C and pH 7. Strain JC656T contained MK-9 as the predominant isoprenoid quinone and anteiso-C15:0, iso-C16:0 and anteiso-C17:0 as the major fatty acids. Diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, phosphatidylmonomethylethanolamine and a glycolipid were the polar lipids. Strain JC656T contained lysine, alanine, glutamine, diaminopimelic acid (DAP) and two unidentified amino acids as characteristic cell wall amino acids. The genome size of strain JC656T was 3.9 Mb with a DNA G + C content of 69.9 mol %. For the affirmation of the strain’s taxonomic status, a detailed phylogenomic study was done. Based on its phylogenetic position and morphological, physiological, and genomic features, strain JC656T represents a new species of the genus Sinomonas, for which we propose the name Sinomonas cellulolyticus sp. nov. The type strain JC656T = (KCTC 49339T = NBRC 114142T).
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Data availability
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of the strain JC656T is ON908988. The GenBank/EMBL/DDBJ accession number for the whole genome shotgun sequence for strain JC656T is NZ_JAERRC01000000.
Abbreviations
- NCBI:
-
National Centre for Biotechnology Information
- gANI:
-
Genome average nucleotide identity
- dDDH:
-
Digital DNA–DNA hybridization
- HPLC:
-
High-pressure liquid chromatography
- KCTC:
-
Korean Collection for Type Cultures
- NBRC:
-
Biological Resource Centre, NITE
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Acknowledgements
Ch Ramana would like to thank the University of Hyderabad, the Institute of Eminence (IoE) and DBT-BILDER for providing financial support.
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This work received a specific grant from the UoH IoE.
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KL performed sample collection, isolated the strain, performed the initial cultivation, strain deposition and strain characterization; KL, JU, SC and CVR performed the genomic and phylogenetic analysis; and KL and SA performed the cell wall analysis. All authors participated in the data finalization, wrote up and approved the final version of the manuscript.
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L. Khongsai, L., Uppada, J., Ahamad, S. et al. Sinomonas cellulolyticus sp. nov., isolated from Loktak lake. Antonie van Leeuwenhoek 116, 1421–1432 (2023). https://doi.org/10.1007/s10482-023-01893-2
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DOI: https://doi.org/10.1007/s10482-023-01893-2