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Luteimonas cellulosilyticus sp. nov., Cellulose-Degrading Bacterium Isolated from Soil in Changguangxi National Wetland Park, China

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Abstract

A Gram-negative, motile, aerobic, and rod-shaped strain (MIC 1.5T) was isolated from soil in Changguangxi national wetland park. Growth occurred at 20–45 °C, at pH 6.0–8.0, and at 0–4.0% NaCl. Based on 16S rRNA gene sequence analysis, strain MIC 1.5T was related to were identified as Luteimonas dalianensis CGMCC 1.12191T (95.3%), Luteimonas padinae DSM 101536T (94.5%), Luteimonas huabeiensis DSM 26429T (94.1%), and Luteimonas mephitis DSM 12574T (92.5%). The DNA-DNA relatedness values between strain MIC 1.5T , and these strains were well below 31%. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The DNA G+C content of strain MIC 1.5T was 66.3 mol%. Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC 1.5T and L. dalianensis CGMCC 1.12191T were 65.39% and 29.52%, respectively. The quinone was identified as Q-8. The major fatty acids were iso-C15:0, iso-C15:0 3OH, and iso-C17:0 3OH and summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH). Based on the phylogenetic, physiological, and chemotaxonomic results, strain MIC 1.5T represents a novel species of the genus Luteimonas, for which the name Luteimonas cellulosilyticus sp. nov. is proposed. The type strain is MIC 1.5T (= KACC 19469T = CCTCC AB 2017256T).

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Acknowledgements

This work was financially supported by Research Program of State Key Laboratory of Food Science and Technology, Jiangnan University (No. SKLF-ZZA-201906), and Fundamental Research Funds for the Central Universities (Nos. JUSRP11968 and JUSRP11961)

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Jiangnan University, 1800 Lihu Ave, Wuxi, 21422, China

    Pei Lin

  2. State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Ave, Wuxi, 21422, China

    Zheng-Fei Yan

  3. Engineering Research Center of Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, China

    Chang-Tian Li

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  1. Pei Lin
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Correspondence to Pei Lin or Zheng-Fei Yan.

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The authors declare no conflicts of interest. The responsible authorities of Changguangxi national wetland park declare no conflicts of interest in soil sample obtained from park.

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Lin, P., Yan, ZF. & Li, CT. Luteimonas cellulosilyticus sp. nov., Cellulose-Degrading Bacterium Isolated from Soil in Changguangxi National Wetland Park, China. Curr Microbiol 77, 1341–1347 (2020). https://doi.org/10.1007/s00284-020-01934-3

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